Proteomic analysis of urinary exosome of pediatric kidney diseases

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15523
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: The University of Tokyo
• Principal Investigator: Harita Yutaka 東京大学, 医学部附属病院, 講師 (10451866)
• Co-Investigator(Kenkyū-buntansha): 神田 祥一郎 東京大学, 医学部附属病院, 助教 (60632651)
• Co-Investigator(Renkei-kenkyūsha): UEDA Koji がん研究会ゲノムセンター, ゲノムセンター, プロジェクトリーダー (10509110) ; HATTORI Motoshi 東京女子医科大学, 医学部, 教授 (50192274) ; MIURA Kenichiro 東京女子医科大学, 医学部, 準教授 (70408483) ; HAMADA Riku 東京都立小児総合医療センター, 体の専門診療部, 医員 (40608783) ; HAMASAKI Yuko 東邦大学, 医学部, 講師 (50317799)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: エクソソーム / ネフロン癆 / 先天性腎尿路異常

Outline of Final Research Achievements

Because there is no urinary biomarker for congenital anomalies of kidney and urinary tract (CAKUT) or nephronophthisis, their diagnosis is often made after irreversible deterioration of renal function. In this study, we aimed at identification of biomarker for pediatric kidney diseases by analyzing urinary exosome by quantitative proteomics. We collected urine samples of patients with nephronophtisis, CAKUT or vesicoureteral reflux, and purified exosome fraction from urine for proteomic anlaysis. The results will reveal the diagnostic marker and may pave the way to unravel the mechanism by which pediatric kidney diseases develop and progress.

Free Research Field

小児科学