Genome-wide Screening of Novel Genes Responsible for Tumor Radioresistance

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15576
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Kyoto University
• Principal Investigator: Harada Hiroshi 京都大学, 放射線生物研究センター, 教授 (80362531)
• Co-Investigator(Kenkyū-buntansha): 吉村 通央 京都大学, 医学研究科, 講師 (40597936) ; 後藤 容子 京都大学, 医学研究科, 特定助教 (60755651)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: がん / 放射線 / 治療抵抗性 / 責任遺伝子 / スクリーニング

Outline of Final Research Achievements

Through a genetic screening experiment, we successfully identified novel genes which potentially induce radioresistance of cancer cells. Clonogenic cell survival assay with the use of their expression vector confirmed that some of the genes actually increase radioresistance of cancer cells. We revealed mechanisms of action of one of the genes; it increased the levels of an intracellular antioxidant, GSH, for radioresistance. IHC analysis for clinical tumor samples found positive correlation between the expression levels of the gene product and poor prognosis of cancer patients.

Free Research Field

放射線腫瘍生物学