Investigation for aspiration pneumonia severity control method using rice-derived protease inhibitors

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15785
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Niigata University
• Principal Investigator: YUTAKA Terao 新潟大学, 医歯学系, 教授 (50397717)
• Co-Investigator(Kenkyū-buntansha): 谷口 正之 新潟大学, 自然科学系, 教授 (00163634) ; 土門 久哲 新潟大学, 医歯学系, 助教 (00594350) ; 前川 知樹 新潟大学, 医歯学系, 研究准教授 (50625168) ; 小田 真隆 京都薬科大学, 薬学部, 教授 (00412403)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 炎症制御

Outline of Final Research Achievements

In order to evaluate the anti-inflammatory effect of bacterial infection of rice derived from rice, bacterial LPS was added to mouse macrophage-derived RAW 264.7 cells pretreated with peptide, and the transcriptional activity of inflammatory cytokines was measured by Real-time PCR And analyzed. Transcription activity of related factors was also measured. Next, in order to verify the inflammatory control effect of the peptide, in in vitro system using RAW 264.7 cells, the rice derived from the rice increased the transcription activity of osteoclast differentiation factor by LPS-induced inflammation and RANKL addition Respectively. It was suggested that the rice peptides selected in this study could have a defense effect against the inflammation caused by bacterial infection.

Free Research Field

歯学