Development of a method for the endochondral alveolar bone tissue engineering using iPS cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15797
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tohoku University
• Principal Investigator: Egusa Hiroshi 東北大学, 歯学研究科, 教授 (30379078)
• Co-Investigator(Kenkyū-buntansha): 鎌野 優弥 東北大学, 大学病院, 助教 (70757260)
• Co-Investigator(Renkei-kenkyūsha): SAITO Masahiro 東北大学, 大学院歯学研究科, 教授 (40215562) ; FUKUMOTO Satoshi 東北大学, 大学院歯学研究科, 教授 (30264253)
• Research Collaborator: OKAWA Hiroko 東北大学, 大学院歯学研究科, 非常勤講師 (00781296) ; KOSAKA Yukihiro 東北大学, 大学院歯学研究科, 大学院生
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 再生歯科補綴学 / iPS細胞 / 軟骨内骨化

Outline of Final Research Achievements

A strategy of induced pluripotent stem (iPS) cell-based alveolar bone regeneration is expected to improve treatment outcome in prosthetic treatments. We focused on possible advantage in endochondral bone regeneration in the large bone defect. The aim of this study was to develop a method to fabricate bone/cartilage-like cell constructs using iPS cells. We found that combination use of chondrogenic and osteogenic induction factors could guide mouse iPS cell embryoid bodies into hybrid bone/cartilage-like cell constructs on the manipulation of a ratio in degrees of chondrocyte/osteoblast differentiation.

Free Research Field

歯科補綴学