Establishment of a novel and easy in vitro toxicity evaluation system using freezed cell spheroid

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15815
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: Kagami Hideaki 松本歯科大学, 総合歯科医学研究所, 教授 (80242866)
• Co-Investigator(Kenkyū-buntansha): 李 憲起 松本歯科大学, 歯学部, 講師 (60350831)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: in vitro試験 / 細胞毒性 / 線維芽細胞 / 凍結保存

Outline of Final Research Achievements

The purpose of this study was to develop a in vitro test for cell toxicity using a novel cell freezing technology. Mouse skin-derived fibroblasts were used. The cells cultured on a regular dish could not survive, On the other hand, the spheroids from fibroblasts could survive and proliferate after freeze-thaw cycle. Then, various freezing conditions including spheroid size, the concentration of cryoprotectant, and the volume of cryoprotectact were optimized. From this study, we could successfully achieved the in vitro cell toxicity test system using spheroids, which can be used immediately after thaw with culture medium without washing process.

Free Research Field

再生医療