Identification of novel oral squamous cell carcinoma-related genes for oder-made treatment.

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K15831
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: Shumozato Kazuo 愛知学院大学, 歯学部, 非常勤講師 (00158966)
• Co-Investigator(Kenkyū-buntansha): 野本 周嗣 愛知学院大学, 歯学部, 教授 (40300967) ; 宮部 悟 愛知学院大学, 歯学部, 講師 (40534582)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 口腔扁平上皮癌 / 口腔白板症 / 口腔細菌叢 / 唾液検体 / 次世代シークエンサ

Outline of Final Research Achievements

Recently, the possibility that oral microbiomes are associated with oral squamous cell carcinoma (OSCC) initiation and progression has attracted attention; however, this association is still unclear. Here, we comprehensively analyze the microbiome profiles of saliva and tissue samples using next-generation sequencing followed by determining the association between oral microbiome profiles and OSCC to detect a novel OSCC biomarker for diagnosis, prognosis and sensitivity of treatment.The results suggesting a strong association of these bacteria with OSCC. Especially, phylum Fusobacterium was significantly associated with early recurrence of OSCC. Thus, oral microbiome analysis may have a potential of novel OSCC detection and prognostic tool.

Free Research Field

口腔外科　腫瘍学

Academic Significance and Societal Importance of the Research Achievements

口腔扁平上皮癌（OSCC）の大部分は肉眼的に発見可能であるが進行癌として診断されることも少なくない。そのことが予後不良の一因となっており、早期発見のための信頼性の高い簡便な診断マーカの開発が課題である。本研究では、採取が簡便で臨床応用しやすい唾液検体の細菌叢解析を行い、口腔細菌の臨床的な意義を検討した。結果、OSCCでのFusobacteria門、Fusobacterium属の増加やFirmicutes門、Streptococcus属の減少がOSCC診断に応用できる可能性が示され、Fusobacteria門やFusobacterium属の割合増加が予後増悪因子となる可能性が示唆された。