Development of highly sensitive hyperspectral imaging method

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K17918
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Shimada Rintaro 東京大学, 大学院理学系研究科(理学部), 特任助教 (70548940)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 分光画像計測 / 蛍光分光 / ラマン分光

Outline of Final Research Achievements

A new hyperspectral imaging spectrometer has been developed. The newly designed system is based on a conventional confocal microscope with additional two galvano-mirror scanner units, one for the excitation laser, and the other for the signal light. By driving the scanner units synchronously, simultaneous detection of signal light from multiple spots on the sample on a charge-coupled-device (CCD) detector has been realized. Hyperspectral imaging of cultured cells and bio-tissue was demonstrated to show fast (12 msec / spectrum) and high sensitive spectral acquisition capability of the system. Owing to its spectral information, localization of multiple biomolecules in a sample was visualized simultaneously with the spatial resolution of the images comparable to that obtained by conventional confocal microscopes.

Free Research Field

分析化学