Study of molecular mechanism of complex brain by ferret

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18382
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yuji Tsunekawa 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 研究員 (80733352)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 複雑脳発生

Outline of Final Research Achievements

The purpose of this project is to understand molecular mechanism of development of the complex gyrencephalic brain. Mice, which is most used model animals to study neural development but they dont't have gyruses in their brain. Because of this fact, I used the ferret as the model animal of this study. Ferret is a samll handy animals which have gyrencephalic brain, however, it is still difficult to apply genome editing technology to ferret. To solve this problem and progress this project, I reported novel de novo targeted gene knock-in techniques mediated by in utero electroporation and Adeno-associated virus (AAV) into the developing and developed complex brain. Further study to understand molecular mechanism of development of the complex gyrencephalic brain will be required by these newly developed technology.

Free Research Field

神経発生学