2017 Fiscal Year Final Research Report
Development of disease-modeling technology using hierarchical genome/epigenome editing
| Project/Area Number |
16K18478
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | がん / ゲノム編集 / エピゲノム編集 / マイクロホモロジー媒介性末端結合 / MMEJ / ゲノム生物工学 / CRISPR-Cas9 / TALEN |
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| Outline of Final Research Achievements |
In this research, we developed the genome/epigenome editing technologies enabling disease modeling caused by genomic and/or epigenomic abnormality that multiplexly occurs in a single cell. Regarding genome editing, we achieved the improvement of MMEJ-dependent gene knock-in (PITCh) and the development of automated design tool of PITCh knock-in. In addition, we realized simultaneous gene knock-in into multiple gene loci using the improved PITCh system. Regarding epigenome editing, we constructed the TREE system for hyper accumulation of effector molecules, showing superior editing effect compared to the conventional systems. Collectively, the technology capable of modeling complex multifactorial pathogenesis was established in terms of both genome and epigenome editing.
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| Free Research Field |
ゲノム生物工学
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