Analysis of the mechanism to regulate protein localization by EPA and DHA

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18755
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Yamada Hidetoshi 公益財団法人岩手生物工学研究センター, 生物資源研究部, 主任研究員 (70511955)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: EPA / DHA / メラノーマ細胞 / メラニン / 腫瘍増殖 / チロシナーゼ / F-Actin

Outline of Final Research Achievements

In this study, we studied the mechanism of anti-melanogenic and anti-tumorgrowth effects of EPA and DHA. We revealed that 1) EPA decreased Tyrosinase and Tyrosinase related protein 1 that located on membrane, 2) DHA delayed the cell cycle of melanoma that involved G2/M phase cell increase. To reveal these mechanism, we examined the protein expression and localization of small GDP-binding proteins. However, we could not observe any difference whether EPA or DHA treated. Therefore, we analyzed cytoskeleton protein, and observed fiber actin was decreased by EPA treated. Our results suggested that a new mechanism of anti-melanogenic and anti-tumorgrowth effects of EPA and DHA via decreasing of F-actin was exist.

Free Research Field

脂質生化学