Aggregation of amyloid-beta protein on size-controlled lipid nanoparticles

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18860
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: University of Toyama
• Principal Investigator: Ikeda Keisuke 富山大学, 大学院医学薬学研究部(薬学), 准教授 (00553281)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: アルツハイマー病 / アミロイド / 脂質膜 / ナノ粒子

Outline of Final Research Achievements

Conversion of amyloid-β (Aβ protein from a non-toxic monomer into the toxic aggregates is the possible pathogenic pathways in Alzheimer’s disease. Recent studies have suggested that lipid membranes play key roles in protein aggregation. However, the binding modes and the mechanisms of Aβ aggregation on lipid vesicles are not fully understood. Here, we prepared a size-controlled lipid nanoparticles in order to control the number of Aβ molecules on the lipid bilayer surfaces of the particles. We have found that the secondary structure and the aggregation propensity of Aβ depended on the size of nanoparticles, suggesting the formation of intermediate species in the aggregation pathway.

Free Research Field

生物物理化学