2017 Fiscal Year Final Research Report
The role of Aeromonas sobria serine protease in the Aeromonas infection and its molecular epidemiological analysis
| Project/Area Number |
16K18926
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Hiroshima International University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | Aeromonas / プロテアーゼ / 上皮バリア / 基質特異性 / 分子疫学 |
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| Outline of Final Research Achievements |
Aeromonas sobria is known as a causative agent evoking gastroenteritis. In severe cases, however, extra-intestinal diseases such as sepsis may occur especially in compromised host. To clarify the role of the extracellular serine protease produced by A. sobria (ASP) in the development of extra-intestinal infection, we investigated the effect of ASP on intestinal tissue using cultured cells. We further studied the substrate specificity of ASP and also examined the productivity of ASP among several strains. As a result, the following findings were obtained. 1) ASP acts on intestinal cell-cell adhesion factors such as nectin-2 and afadin, and destroys the epithelial barrier function of intestinal tissue., 2) We found that the Arg-566 residue of ASP plays an important role in molecular recognition of substrate., 3) We established an experimental system to investigate the ASP production and genotype of various Aeromonas strains.
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| Free Research Field |
腸管感染細菌由来病原因子の機能解析
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