Molecular mechanism analysis of intracellular drug delivery by fatty acid-binding protein

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K18963
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: Yamamoto Atsushi 鈴鹿医療科学大学, 薬学部, 助手 (90633991)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 脂肪酸結合タンパク質（FABP） / 薬物結合 / 細胞内輸送 / 疎水性薬物

Outline of Final Research Achievements

While the drug-binding proteins in blood plasma such as albumin and alpha1-acid glycoprotein have been investigated in detail, the proteins in cells have been hardly studied. In this study, we focused on that the fatty acid-binding protein (FABP) binds various hydrophobic drugs and analyzed using E. coli expression system and cultured cells. As a result, we succeeded in identifying a drug that newly binds to FABP, comparing drug binding abilities among FABP isoforms, and constructing a drug uptake assay using cultured cells.

Free Research Field

薬剤学、分子生物学

Academic Significance and Societal Importance of the Research Achievements

多くの薬物は生体内では基本的にタンパク質と結合して運搬されており、この結合は薬効発現や副作用惹起と深く関わる。本研究では、脂肪酸結合タンパク質（FABP）が細胞内における薬物結合タンパク質としての役割を解明し、細胞内薬物動態学という新たな学術分野を切り開くとともに、薬物の作用発現や副作用惹起のメカニズムを解き明かすことを目指す。今回、FABPに結合する薬物の同定やその特徴付け、培養細胞を用いた解析を行い、FABPの薬物結合研究における基盤を築いた。