2018 Fiscal Year Annual Research Report
Development of new methods for measuring ion concentrations in interstitial fluid using optical spectroscopy
| Project/Area Number |
16K18990
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| Research Institution | Kanazawa University |
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Principal Investigator |
プップリン レオナルド 金沢大学, ナノ生命科学研究所, 特任助教 (40771580)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Raman spectroscopy / pH / Gold nanoparticles / Cancer cell |
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| Outline of Annual Research Achievements |
We used of gold nanoparticles (AuNP) to create a high spatial resolution sensor for measuring extracellular pH in proximity of the cell membrane. We test the sensor on HepG2 liver cancer cells and MKN28 gastric cancer cells before and after inhibition of Na+/H+ exchanger. The gold surface conjugation strategy was conceived with a twofold purpose: i) to anchor the AuNP to the membrane proteins and ii) to quantify the local pH from AuNP using surface enhanced Raman spectroscopy (SERS). The nanometer size of the cell membrane anchored sensor and the use of SERS enable us to visualize highly localized variation of pH induced by H+ extrusion, which is particularly upregulated in cancer cells. The results are published on Nature Communications, 9, Article number: 5278 (2018).
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