2018 Fiscal Year Final Research Report
Development of new methods for measuring ion concentrations in interstitial fluid using optical spectroscopy
| Project/Area Number |
16K18990
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Kanazawa University (2018)
Kyoto Prefectural University of Medicine (2016-2017) |
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Principal Investigator |
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| Research Collaborator |
Hosogi Shigekuni
Sun Hongxin
Matsuo Kazuhiko
Marunaka Yoshinori
Inui Toshio
Tanaka Hideo
Kumamoto Yasuaki
Suzaki Toshinobu
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Raman spectroscopy / pH / interstitial fluid / gold nanoparticles |
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| Outline of Final Research Achievements |
We exploited the remarkable optical properties of gold nanoparticles and their ability to conjugate with different thiol-containing molecular compounds to develop a method for highly localized pH bio-sensing using Raman spectroscopy. The strategy for gold nanoparticles conjugation was specifically designed to efficiently target the cell membrane proteins and to quantify the local pH by collecting the Raman scattering of the 4-MBA monolayer assembled on the gold surface. Experiments on HepG2 human liver cancer cells and MKN28 gastric cancer cells proved the successful anchoring of the gold nanoparticles to the outer cell membrane and showed substantial acidification of the extracellular surface pH. The proposed method of analysis can be used as a simple and viable tool to investigate and unfold the dynamics of proton exchange in cells, even after exposure to different pharmacological treatments or different physiological conditions.
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| Free Research Field |
Cell physiology
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| Academic Significance and Societal Importance of the Research Achievements |
Dysregulated pH is a common characteristic of cancer cells compared with normal cells. The precise measurement of extracellular pH on the cell membrane using Raman spectroscopy can be used in the future to characterize primary and metastatic cancer cell populations and minimal residual disease.
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