The understanding of the regulatory mechanisms how MYMLR regulates MYC

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Nagoya University
• Principal Investigator: Kajino Taisuke 名古屋大学, 医学系研究科, 助教 (50723673)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: MYC / lncRNA

Outline of Final Research Achievements

We previously identified the novel long non-coding RNA (lncRNA) MYMLR as a regulator of MYC transcriptional factor in lung cancer. To understand the molecular mechanisms how MYMLR regulates MYC, we performed RNA pull down assay by the usage of BrU-labelled MYMLR and identified MYMLR-binding proteins. In addition, we established ChIRP (Chromatin isolation by RNA purification) assay methods and found that MYMLR bound to MYC promoter. Furthermore, we generated germ line knockout mice of Mymlr and found that Mymlr knockout mice were viable. These results indicated that MYMLR is indispensable for tumor growth but not in normal organs or tissues, suggesting that MYMLR is the potential druggable target for lung cancer.

Free Research Field

分子腫瘍学