Predictive marker for metastasis in NET-G1

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19347
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: The University of Tokushima
• Principal Investigator: KITAMURA Shinji 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (60564490)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 直腸カルチノイド

Outline of Final Research Achievements

We performed miRNA array analysis using 7 NET-G1 tumors with LVI and 7 without LVI. Identified miRNA expression level was evaluated by RT-PCR. Target genes were examined by IPA and TargetScan analyses. A NET cell line H727 transfected with miRNA mimic or target gene siRNA was examined for proliferation, migration and invasion.The expression levels of miR-144-3p and miR-451a in NET-G1 tumors with LVI were significantly higher than those without LVI with miRNA array analysis and RT-PCR. The target gene analyses revealed that miR-144-3p and miR-451a directly interact with PTEN and p19 mRNA, respectively. The miR-451a mimic significantly increased cell migration, and miR-144-3p mimic significantly increased cell invasion. Knockdown of PTEN induced significant augmentation of cell invasion. The miR-144-3p and miR-451a may be associated with metastasis in NET-G1 through repression of PTEN/p19.

Free Research Field

消化器内科