2017 Fiscal Year Final Research Report
Predictive marker for metastasis in NET-G1
Project/Area Number |
16K19347
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | The University of Tokushima |
Principal Investigator |
KITAMURA Shinji 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (60564490)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Keywords | 直腸カルチノイド |
Outline of Final Research Achievements |
We performed miRNA array analysis using 7 NET-G1 tumors with LVI and 7 without LVI. Identified miRNA expression level was evaluated by RT-PCR. Target genes were examined by IPA and TargetScan analyses. A NET cell line H727 transfected with miRNA mimic or target gene siRNA was examined for proliferation, migration and invasion.The expression levels of miR-144-3p and miR-451a in NET-G1 tumors with LVI were significantly higher than those without LVI with miRNA array analysis and RT-PCR. The target gene analyses revealed that miR-144-3p and miR-451a directly interact with PTEN and p19 mRNA, respectively. The miR-451a mimic significantly increased cell migration, and miR-144-3p mimic significantly increased cell invasion. Knockdown of PTEN induced significant augmentation of cell invasion. The miR-144-3p and miR-451a may be associated with metastasis in NET-G1 through repression of PTEN/p19.
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Free Research Field |
消化器内科
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