Role of polycomb histone methyltransferase EZH1 in hematologic malignances

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19565
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Chiba University
• Principal Investigator: Aoyama Kazumasa 千葉大学, 大学院医学研究院, 特任助教 (50734266)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: エピジェネティック制御 / 癌 / 遺伝子発現制御 / ポリコーム / 造血器腫瘍

Outline of Final Research Achievements

Ezh1 and 2 repress gene expression by catalyzing trimethylation at histone H3 lysine 27 (H3K27me3). Here, we examined Ezh1+/-Ezh2Δ/Δ mice. ChIP sequence analysis revealed that a cohort of PRC2 targets marked with higher levels of H3K27me3 than the other targets in hematopoietic stem and progenitor cells (HSPCs), retained high levels of H3K27me3 in Ezh1+/-Ezh2Δ/Δ HSPCs. Moreover, using SKM-1 cells, derived from human MDS patients with EZH2 mutant, we showed that repression of core PRC2 targets is critical for the maintenance of human PRC2-insufficient MDS.

Free Research Field

分子生物学