The role of Toll-like receptor 3 in the pathogenesis of immediate type hypersensitivity

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K19734
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Nakamura Naomi 京都府立医科大学, 医学部附属病院, 研究員 (10733058)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: Toll様受容体 / 即時型アレルギー

Outline of Final Research Achievements

We investigated the role of TLR3 in immediate hypersensitivity in a model of IgE-dependent food anaphylaxis. Wild-type (WT), Tlr3 knockout (KO) mice were epicutaneously sensitized with ovalbumin (OVA) and then challenged orally with OVA. However, there was no significant difference in the serum levels of OVA-specific IgE antibody between OVA-sensitized WT and Tlr3 KO mice. To investigate whether Tlr3 plays a role in the skin barrier dysruption, we mesured IL-33 and TSLP in the tape-stripped mice skin. However, IL-33 and TSLP were not sigmificaly increased. In additoin, the reaction of food anaphylaxis elicited by oral OVA challenge of epicutaneous sensitized WT mice was comparative of that in Tlr3 KO mice.

Free Research Field

皮膚科学