肝細胞癌における癌関連好中球（TAN）の分離解析による役割の解明

2016 Fiscal Year Research-status Report

• Project/Area Number: 16K19936
• Research Institution: Kumamoto University
• Principal Investigator: 岡部 弘尚 熊本大学, 医学部附属病院, 非常勤診療医師 (40573621)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 腫瘍関連好中球 / CD8 T細胞 / 制御性T細胞 / 腫瘍関連マクロファージ / 肝外胆管癌

Outline of Annual Research Achievements

腫瘍関連好中球はこれまでCD8 T cell、Regulatory T cellとの相互作用が報告されており、我々は肝外胆管癌において腫瘍関連マクロファージも加えた腫瘍関連間質構成細胞の免疫組織学的な解析を行った。まず免疫組織の条件を検索し、肝外胆管癌100例における発現解析を行っている。約50例解析時点での結果ではTAN優位な腫瘍はCD8 T cellが少なく、Regulatory T cellが少ない結果である。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

当初の予定通り進んでおり、得られた結果については論文への投稿準備を行っている。

Strategy for Future Research Activity

免疫組織学的な解析をすすめ、腫瘍構成細胞の複数のマーカーを用いたRisk-signatureを定義し（Nakagawa S et al. Cancer Cell 2016）、予後予測における有用性も今後評価する。

Causes of Carryover

医局内保管の試薬・消耗品をしようできたため。

Expenditure Plan for Carryover Budget

研究費は主に試薬等消耗品の購入費に充てる。また、研究成果発表及び情報収集にかかる学会出張旅費に充てたいと考える。

Research Products

• [Journal Article] Shape and enhancement characteristics of pancreatic neuroendocrine tumor on preoperative contrast-enhanced computed tomography may be prognostic indicators. (2017)
  • Author(s): Okabe H, Hashimoto D, Chikamoto A, Morikatsu Y, Taki K, Arima K, Imai K,Tamura Y, Ikeda O, Ishiko T, Uchiyama H, Ikegami T, Harimoto N, Itoh S, Yamashita Y, Yoshizumi T, Beppu T, Yamashita Y, Baba H, Maehara Y
  • Journal Title: Ann Surg Oncol Volume: 24 Pages: 1399-1405
  • DOI: 10.1245/s10434-016-5630-4.
  • Peer Reviewed
• [Journal Article] Neutrophil-to-lymphocyte ratio predicts metachronous liver metastasis of pancreatic neuroendocrine tumors. (2017)
  • Author(s): Arima K, Okabe H, Hashimoto D, Chikamoto A, Nitta H, Higashi T, Kaida T, Yamamura K, Kitano Y, Komohara Y, Yamashita YI, Beppu T, Takeya M, Baba H.
  • Journal Title: Int J Clin Oncol Volume: in press Pages: in press
  • DOI: 10.1007/s10147-017-1111-4.
  • Peer Reviewed
• [Journal Article] Wnt signaling regulates hepatobiliary repair following cholestatic liver injury in mice. (2016)
  • Author(s): Okabe H, Yang J, Sylakowski K, Yovchev M, Miyagawa Y, Nagarajan S, Chikina M, Thompson M, Oertel M, Baba H, Monga SP, Nejak-Bowen KN.
  • Journal Title: Hepatology Volume: 64(5) Pages: 1652-1666
  • DOI: 10.1002/hep.28774.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Diverse basis of β-catenin activation in human hepatocellular carcinoma: Implications in biology and prognosis. (2016)
  • Author(s): Okabe H, Kinoshita H, Imai K, Nakagawa S, Higashi T, Arima K, Uchiyama H, Ikegami T, Harimoto N, Itoh S, Ishiko T, Yoshizumi T, Beppu T, Monga SP, Baba H and Maehara Y.
  • Journal Title: PLoS One Volume: 11(4) Pages: e0152695
  • DOI: 10.1371/journal.pone.0152695
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Portal Vein Embolization Followed by Right-Side Hemihepatectomy for Hepatocellular Carcinoma Patients: A Japanese Multi-Institutional Study. (2016)
  • Author(s): Beppu T, Okabe H, Okuda K, Eguchi S, Kitahara K, Taniai N, Ueno S, Shirabe K, Ohta M, Kondo K, Nanashima A, Noritomi T, Okamoto K, Kikuchi K, Baba H, FujiokaH.
  • Journal Title: J Am Coll Surg Volume: 222(6) Pages: 1138-1148.e2
  • DOI: 10.1016/j.jamcollsurg.2016.03.023.
  • Peer Reviewed
• [Journal Article] The diagnostic role of the neutrophil-to-lymphocyte ratio in predicting pancreatic ductal adenocarcinoma in patients with pancreatic diseases. (2016)
  • Author(s): Arima K, Okabe H, Hashimoto D, Chikamoto A, Tsuji A, Yamamura K, Kitano Y, Inoue R, Kaida T, Higashi T, Taki K, Imai K, Komohara Y, Beppu T, Takeya M, Baba H.
  • Journal Title: Int J Clin Oncol Volume: 21(5) Pages: 940-945
  • DOI: 10.1007/s10147-016-0975-z
  • Peer Reviewed
• [Journal Article] Salvage splenic artery embolization for saving falling living donor graft due to portal overflow. (2016)
  • Author(s): Okabe H, Yoshizumi T, Ikegami T, Uchiyama H, Harimoto N, Itoh S, Kimura K, Baba H, Maehara Y.
  • Journal Title: Transplant Proc. Volume: 48(9) Pages: 3171-3173
  • DOI: 10.1016/j.transproceed.2016.07.023.
  • Peer Reviewed
• [Journal Article] A long survivor with local relapse of hilar cholangiocarcinoma after R1 surgery treated with chemoradiotherapy: a case report and literature review. (2016)
  • Author(s): Okabe H, Chikamoto A, Maruno M, Hashimoto D, Imai K, Taki K, Arima K, Ishiko T, Uchiyama H, Ikegami T, Harimoto N, Itoh S, Yoshizumi T, Beppu T, Baba H, Maehara Y.
  • Journal Title: Surg Case Rep Volume: 2 Pages: 69
  • DOI: 10.1186/s40792-016-0195-9
  • Peer Reviewed
• [Journal Article] A case report of bacteremia manifesting as an overwhelming postsplenectomy infection due to Streptococcus pneumoniae post vaccination. (2016)
  • Author(s): Hirose K, Okabe H, Yoshizumi T, Uchiyama H, Ikegami T, Harimoto N, Itoh S, Kimura K, Baba H, Maehara Y.
  • Journal Title: Surg Case Rep Volume: 2(1) Pages: 48
  • DOI: 10.1186/s40792-016-0173-2.
  • Peer Reviewed
• [Journal Article] Inducible factors for cancer-associated fibroblasts in liver cancer versus myofibroblasts in inflammatory liver disease. (2016)
  • Author(s): Okabe H, Hayashi H, Nakagawa S, Imai K, Nitta H, Arima K, Hashimoto D, Chikamoto A, Ishiko T, Beppu T, Baba H.
  • Journal Title: Histol Histopathol Volume: 31(2) Pages: 141-8
  • DOI: 10.14670/HH-11-668
  • Peer Reviewed