The therapeutic effect of anti-Angionpoietin 2 antibody in EAU

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K20294
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: IWATA DAIJU 北海道大学, 医学研究院, 助教 (70374402)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: ぶどう膜炎 / アンジオポエチン2 / 実験的自己免疫性ぶどう膜網膜炎

Outline of Final Research Achievements

We investigated the effect of angiopoietin (Ang) 2 inhibitor treatment on clinical and histological severity of experimental autoimmune uveoretinitis (EAU). 6-week-old C57BL/6 mice were induced EAU. A group of EAU mice was created in which an anti-Ang2 antibody and a non-specific antibody (control) were administered a total of 5 times every 5 days starting the day before EAU induction, the clinical severity was observed over time (every 3-4 days), and the eyes were removed on day 21 to evaluate histopathological severity. The clinical and histopathological severity of the EAU mice was significantly reduced in the anti-Ang2 group compared to the control group (p<0.05).

Free Research Field

眼科

Academic Significance and Societal Importance of the Research Achievements

本研究では、Ang2の阻害でEAUの臨床的重症度と病理組織学的重症度が低下することを明らかとした。Ang2を阻害したことでAng１優位となり、 内皮細胞上の白血球接着分子が減少し、炎症が軽減したと考えられる。この結果から、Ang2が非感染性ぶどう膜炎の炎症の進展に関与しており、Ang2の阻害が非感染性ぶどう膜炎の治療に有効である可能性が示された。