2017 Fiscal Year Final Research Report
Mechanisms of tumorigenesis through novel BCOR abnormality in clear cell sarcoma of the kidney
Project/Area Number |
16K20349
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pediatric surgery
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Research Institution | National Center for Child Health and Development |
Principal Investigator |
Ueno Hitomi 国立研究開発法人国立成育医療研究センター, 小児血液・腫瘍研究部, 上級研究員 (30435630)
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Research Collaborator |
Okita Hajime 慶応義塾大学, 医学部・病理学教室, 准教授
Takada Shuji 国立研究開発法人 国立成育医療研究センター, システム発生・再生医学研究部, 部長
Nakabayashi Kazuhiko 国立研究開発法人 国立成育医療研究センター, 周産期病態研究部・周産期ゲノミクス研究室, 室長
Kiyokowa Nobutaka 国立研究開発法人 国立成育医療研究センター, 小児血液・腫瘍研究部, 部長
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Keywords | 腎明細胞肉腫 / BCOR / ポリコーム |
Outline of Final Research Achievements |
Clear cell sarcoma of the kidney has a characteristic novel BCOR abnormality; i.e. duplicated sequences at important domain for polycomb repressive complex formation (BCOR internal tandem duplication: BCOR-ITD). We investigated binding ability of BCOR-ITD to BCOR binding proteins. BCOR-ITD was capable to bind BCL6 and PCGF1, both of which are BCOR binding proteins. Therefore, BCOR-ITD could be involved in tumorigenesis by potential different mechanisms from BCOR loss of function mutations, such as frameshift and nonsense mutations. As a result of histone modification analysis of tumor samples using ChIP-sequencing, clear cell sarcoma of the kidney had some distinctive peak regions. The regions were also exhibited unique pattern in DNA methylation analysis.
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Free Research Field |
小児がん
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