Development of new articular cartilage reconstruction method using cartilage / bone tissue-inducible hybrid biomaterial

2019 Fiscal Year Annual Research Report

• Project/Area Number: 16K20577
• Research Institution: Okayama University
• Principal Investigator: 高畠 清文 岡山大学, 医歯薬学総合研究科, 助教 (70736537)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 人工生体材料 / 幾何学的構造 / ハニカムTCP / 骨組織形成 / 軟骨組織形成

Outline of Annual Research Achievements

本研究では、骨・軟骨組織をシームレスに連続した状態で誘導可能な軟骨・骨組織誘導性ハイブリッド生体材料の構築を目的にハニカムTCPを用いた実験を行った。形状の異なるハニカムTCPを用いて軟骨・骨組織を特異的に誘導した結果、直線的貫通孔の孔径75μmのハニカムTCPで軟骨形成を認めた。孔径300μmのハニカムTCPでは骨形成を認めた。以上のことから、ハニカムTCPの形状を変化させることにより軟骨・骨組織を特異的に誘導することができ、これらをシームレスに連続させることにより関節軟骨を再生できる新規関節軟骨再建法の開発に繋がる可能性が示唆された。
研究期間全愛を通じての研究成果としては、ウサギ飛騨関節欠損モデルを作製し、孔径300μmのハニカムTCPを埋入すると、ウサギ膝関節骨組織相当部にはハニカムTCP孔内に骨組織形成を認め、また膝関節の軟骨組織相当部にはサフラニンレッドに染色される軟骨組織形成を認めた。

Research Products

• [Journal Article] Feasible Advantage of Bioactive/Bioresorbable Devices Made of Forged Composites of Hydroxyapatite Particles and Poly-L-lactide in Alveolar Bone Augmentation: A Preliminary Study (2019)
  • Author(s): Shintaro Sukegawa, Hotaka Kawai, Keisuke Nakano, Takahiro Kanno, Kiyofumi Takabatake, Hitoshi Nagatsuka, Yoshihiko Furuki
  • Journal Title: Int J Med Sci. Volume: 16(2) Pages: 318-323
  • DOI: doi: 10.7150/ijms.27986.
  • Peer Reviewed / Open Access
• [Journal Article] Nicotine promotes lymph node metastasis and cetuximab resistance in head and neck squamous cell carcinoma. (2019)
  • Author(s): Shimizu R, Ibaragi S, Eguchi T, Kuwajima D, Kodama S, Nishioka T, Okui T, Obata K, Takabatake K, Kawai H, Ono K, Okamoto K, Nagatsuka H, Sasaki A.
  • Journal Title: Int J Oncol. Volume: 54(1) Pages: 283-294
  • DOI: doi: 10.3892/ijo.2018.4631.
  • Peer Reviewed / Open Access
• [Journal Article] In Vivo Tissue response of Endodontic Bio-ceramic materials (2019)
  • Author(s): Teresita Romano, Mara Victoria Jammal, Keisuke Nakano, Ana Garca Rusco, Jorge Olmos Fassi, Silvia Kozuszko, Kiyofumi Takabatake, Hitoshi Nagatsuka, Liliana Raquel Missana
  • Journal Title: Journal of Hard Tissue Biology Volume: 28(1) Pages: 1-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Notch Signaling Affects Oral Neoplasm Cell Differentiation and Acquisition of Tumor-Specific Characteristics. (2019)
  • Author(s): Nakano K, Takabatake K, Kawai H, Yoshida S, Maeda H, Kawakami T, Nagatsuka H.
  • Journal Title: Int J Mol Sci. Volume: 20(8) Pages: E1973
  • DOI: doi: 10.3390/ijms20081973.
  • Peer Reviewed / Open Access
• [Journal Article] Biomechanical Loading Comparison between Titanium and Unsintered Hydroxyapatite/Poly-L-Lactide Plate System for Fixation of Mandibular Subcondylar Fractures. (2019)
  • Author(s): Sukegawa S, Kanno T, Yamamoto N, Nakano K, Takabatake K, Kawai H, Nagatsuka H, Furuki Y.
  • Journal Title: Materials Volume: 12(9) Pages: E1557
  • DOI: doi: 10.3390/ma12091557.
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemistry of YAP and dNp63 and survival analysis of patients bearing precancerous lesion and oral squamous cell carcinoma. (2019)
  • Author(s): Ono S, Nakano K, Takabatake K, Kawai H, Nagatsuka H.
  • Journal Title: Int J Med Sci. Volume: 16(5) Pages: 766-773
  • DOI: doi: 10.7150/ijms.29995.
  • Peer Reviewed / Open Access
• [Journal Article] Tumor Angiogenic Inhibition Triggered Necrosis (TAITN) in Oral Cancer. (2019)
  • Author(s): Yoshida S, Kawai H, Eguchi T, Sukegawa S, Oo MW, Anqi C, Takabatake K, Nakano K, Okamoto K, Nagatsuka H.
  • Journal Title: Cells Volume: 8(7) Pages: E761
  • DOI: doi: 10.3390/cells8070761.
  • Peer Reviewed / Open Access
• [Journal Article] Differentiation and Roles of Bone Marrow-Derived Cells on Tumor Microenvironment in Oral Squamous Cell Carcinoma. (2019)
  • Author(s): Chang Anqi, Kiyofumi Takabatake, Hotaka Kawai, May Wathone Oo, Saori Yoshida, Masae Fujii, Haruka Omori, Shintaro Sukegawa, Keisuke Nakano, Hidetsugu Tsujigiwa, Zheng Jinhua, Hitoshi Nagatsuka.
  • Journal Title: Oncology Letters Volume: 18 Pages: 6628-6638
  • DOI: doi: 10.3892/ol.2019.11045.
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Advantage of Alveolar Ridge Augmentation with Bioactive/Bioresorbable Screws Made of Composites of Unsintered Hydroxyapatite and Poly-L-lactide. (2019)
  • Author(s): Sukegawa S, Kawai H, Nakano K, Takabatake K, Kanno T, Nagatsuka H, Furuki Y.
  • Journal Title: Materials Volume: 12(22) Pages: E3681
  • DOI: doi: 10.3390/ma12223681.
  • Peer Reviewed / Open Access
• [Journal Article] Do the Presence of Mandibular Third Molar and the Occlusal Support Affect the Occurrence and the Mode of Mandibular Condylar Fractures? (2019)
  • Author(s): Shintaro Sukegawa, Masato Saika, Takahiro Kanno, Keisuke Nakano, Kiyofumi Takabatake, Hotaka Kawai, Hitoshi Nagatsuka, Yoshihiko Furuki
  • Journal Title: Journal of Hard Tissue Biology Volume: 28(4) Pages: 377-382
  • Peer Reviewed / Open Access
• [Journal Article] The Role of Sonic Hedgehog Signaling in the Tumor Microenvironment of Oral Squamous Cell Carcinoma. (2019)
  • Author(s): Takabatake K, Shimo T, Murakami J, Anqi C, Kawai H, Yoshida S, Wathone Oo M, Haruka O, Sukegawa S, Tsujigiwa H, Nakano K, Nagatsuka H.
  • Journal Title: Int J Mol Sci. Volume: 20(22) Pages: E5779
  • DOI: doi: 10.3390/ijms20225779.
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ハニカムTCPの硬組織形成制御機構解明と臨床応用 (2019)
  • Author(s): 大森悠加、高畠清文、辻極秀次、河合穂高、吉田沙織、May wathone Oo、中野敬介、長塚仁
  • Organizer: 第40回岡山歯学会総会・学術大会
• [Presentation] 頭頸部領域再建におけるハニカムTCPの硬組織再生メカニズム解明と臨床応用 (2019)
  • Author(s): 高畠清文、辻極秀次、河合穂高、吉田沙織、大森悠加、中野敬介、川上敏行、長塚仁
  • Organizer: 第28回硬組織再生生物学会学術大会・総会
• [Presentation] ハニカムTCPの幾何学的構造による血管新生を介した選択的骨・軟骨形成制御 (2019)
  • Author(s): 高畠清文、辻極秀次、浜田芽衣、河合穂高、吉田沙織、大森悠加、中野敬介、長塚仁
  • Organizer: 第73回NPO法人 日本口腔科学会学術集会