2017 Fiscal Year Final Research Report
Binding of ribosomal RNA genes to the nuclear periphery in budding yeast
| Project/Area Number |
16K20987
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
Cell biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | リボソームRNA遺伝子 / 核膜 / 老化 |
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| Outline of Final Research Achievements |
The ribosomal RNA genes (rDNA) in budding yeast are organized into a single tandem array of about 150 repeats. Fob1 unidirectionally inhibits replication fork progression at the replication fork barrier and induces DNA double-strand break (DSB) in the rDNA. The DSB leads to unequal sister-chromatid recombination and hence rDNA instability.
We performed chromatin immunoprecipitation assay to determine the localization of rDNA within the nucleus. We showed that rDNA binds to the nuclear pore in a manner dependent on Fob1 and DNA damage checkpoint kinase Tel1. The factors which are required for the rDNA-nuclear envelope binding are also important for the rDNA stability. We speculate that Fob1 sequesters rDNA at the nuclear periphery to inhibit aberrant recombination events.
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| Free Research Field |
分子細胞生物学
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