2017 Fiscal Year Final Research Report
The pathogenesis study in rats with delayed carbon monoxide encephalopathy
| Project/Area Number |
16K21207
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Psychiatric science
Emergency medicine
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| Research Institution | Ehime University |
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Principal Investigator |
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| Research Collaborator |
Ueno Shuichi 愛媛大学, 大学院医学系研究科, 教授 (80232768)
Iga Jun-ichi 愛媛大学, 大学院医学系研究科, 准教授 (70363140)
Nishihara Tasuku 愛媛大学, 医学部附属病院, 助教 (50568912)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 間歇型一酸化炭素中毒 / ミクログリア / アストロサイト / 神経幹細胞 |
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| Outline of Final Research Achievements |
Rats were exposed to carbon monoxide (CO) until they lost consciousness. Behavioral effects on learning and memory function were measured by the passive-avoidance test until 3 weeks. The latencies were significantly shorter in the CO models.
Immunohistochemical analyses revealed cell numbers in SOX2 positive cells and microglia tended to decrease CO models less than controls in the dentate gyrus. Especially, a lot of morphologically abnormal microglia was found. On the other hand, astrocytes tends to increase CO models more than controls in the dentate gyrus. Flow cytometry analyses revealed that the cell numbers of microglia were significantly reduced in CO models. These results suggested that delayed CO encephalopathy may occur the abnormalities in glial cells such as microglia and astrocytes, and reduce neural precursor cells. Thus, the impairment of neural precursor cells via abnormalities of glial cells may be affected in the mechanism of delayed neuronal injury.
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| Free Research Field |
精神医学
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