2009 Fiscal Year Final Research Report
Functions of RecQ family helicases in DNA replication, repair, and damage avoidance
Project/Area Number |
17013005
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | Tohoku University |
Principal Investigator |
ENOMOTO Takemi Tohoku University, 大学院・薬学研究科, 教授 (80107383)
|
Project Period (FY) |
2005 – 2009
|
Keywords | 癌 / ブルーム症候群 / ロスモンド・トムソン症候群 / RecQファミリーへリカーゼ / DNA複製 / DNA組換え / 姉妹染色分体接着 |
Research Abstract |
Human cells possess five RecQ family helicases, RECQL1, BLM, WRN, RTS, and RECQL5, whose defects cause a predisposition to cancer. In this study, functions of these RecQ family helicases were investigated and following results were obtained. BLM functions at the last stage of DNA replication, segregation of replicated DNA and sister chromatid cohesion. RTS is involved in DNA replication and repair of DNA double strand breaks. Finally, RECQL1 and RECQL5 are involved in early stages of recombination.
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[Journal Article] Global analysis of mutual interaction surfaces of nucleosomes with comprehensive point mutants2009
Author(s)
Sakamoto, M., Noguchi, S., Kawashima, S., Okada, Y., Enomoto, T., Seki, M., Horikoshi, M
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Journal Title
Genes Cells 14
Pages: 1271-1330
Peer Reviewed
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[Journal Article] Structure of the Cdt1 C-terminal domain: conservation of the winged helix fold in replication licensing factors2009
Author(s)
Khayrutdinov, B.I., Bae, W.J., Yun, Y.M., Lee, J.H., Tsuyama, T., Kim. J.J., Hwang, E., Ryu, K.S., Cheong, H.K., Cheong, C., Ko, J.S., Enomoto, T., Karplus, P.A., Guntert. P., Tada, S., Jeon, Y.H., Cho, Y
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Journal Title
Protein Sci 18
Pages: 2252-2264
Peer Reviewed
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[Journal Article] Effect of heliquinomycin on the activity of human MCM4/6/7 helicase2009
Author(s)
Ishimi, Y., Sugiyama, T., Nakaya, R., Kanamori, M., Kohno, T., Enomoto, T., Chino, M
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Journal Title
FEBS J 276
Pages: 3382-3391
Peer Reviewed
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[Journal Article] Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication2009
Author(s)
Ohuchi, T., Seki, M., Kugou, K., Tada, S., Ohta, K., Enomoto, T
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Journal Title
DNA Repair 8
Pages: 690-696
Peer Reviewed
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[Journal Article] Alternative title: Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD22009
Author(s)
Takada, S., Inoue, E., Tano, K., Yoshii, H., Abe, T., Yoshimura, A., Akita, M., Tada, S., Watanabe, M., Seki, M., Enomoto, T
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Journal Title
Biochem. Biophys. Res. Commun 379
Pages: 233-238
Peer Reviewed
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[Journal Article] Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts2009
Author(s)
Tsuyama, T., Watanabe, S., Aoki, A., Cho, Y., Seki, M., Enomoto, T., Tada, S
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Journal Title
Mol. Bio. Cell 20
Pages: 937-947
Peer Reviewed
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[Journal Article] A novel function for Rad17 in homologous recombination2008
Author(s)
Nishino, K., Inoue, E., Takada, S., Abe, T., Akita, M., Yoshimura, A., Tada, S., Kobayashi, M., Yamamoto, K., Enomoto, T
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Journal Title
Genes Genet. Syst 83
Pages: 427-431
Peer Reviewed
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[Journal Article] RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability2008
Author(s)
Xu, D., Guo, R., Sobeck, A., Bachrati, C.Z., Yang, J., Enomoto, T., Brown, G.W., Hoatlin, M.E., Hickson, I.D., Wang W
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Journal Title
Gene Dev 22
Pages: 2843-2855
Peer Reviewed
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[Journal Article] KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation2008
Author(s)
Abe, T., Ishiai, M., Hosono, Y., Yoshimura, A., Tsuji, H., Tada, S., Adachi, N., Koyama, H., Takata, M., Takeda, S., Enomoto, T., Seki, M
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Journal Title
Cell Signal 20
Pages: 1978-1985
Peer Reviewed
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[Journal Article] Rad52 sumoylation and its involvement in the efficient induction of homologous recombination2008
Author(s)
Ohuchi, T., Seki, M., Branzei, D., Maeda, D., Ui, A., Ogiwara, H., Tada, S., Enomoto, T
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Journal Title
DNA Repair 7
Pages: 879-889
Peer Reviewed
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[Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase III・2008
Author(s)
Otsuki, M., Seki, M., Inoue, E., Abe. T., Narita, Y., Yoshimura, A., Tada, S., Ishii, Y., Enomoto, T
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Journal Title
Biochim. Biophys. Acta 1782
Pages: 75-8
Peer Reviewed
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[Journal Article] Functional interactions between BLM and XRCC3 in the cell2007
Author(s)
Otsuki, M., Seki, M., Inoue, E., Yoshimura, A., Kato, G., Yamanouchi, S., Kawabe, Y., Tada, S., Shinohara, A., Komura, J., Ono, T., Takeda, S., Ishii, Y., Enomoto, T
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Journal Title
J. Cell Biol 179
Pages: 53-63
Peer Reviewed
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[Journal Article] Ctf18 is required for homologous recombination -mediated double strand break repair2007
Author(s)
Ogiwara, H., Ohuchi, T., Ui, A., Tada, S., Enomoto, T., Seki, M
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Journal Title
Nuc. Acids Res 35
Pages: 4989-5000
Peer Reviewed
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[Journal Article] Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells2007
Author(s)
Ui, A., Seki, M., Ogiwara, H., Lai, M.S., Yamamoto, K., Tada, S., Enomoto, T
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Journal Title
Biochem. Biophys. Res. Commun 356
Pages: 1031-1037
Peer Reviewed
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[Journal Article] Actin-related protein Arp4 functions in kinetochore construction2007
Author(s)
Ogiwara, H., Ui, A., Kawashima, S., Kugou, K., Onoda, F., Iwahashi, H., Harata, M., Ohta, K., Enomoto, T., Seki, M
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Journal Title
Nuc. Acids Res 35
Pages: 3109-3117
Peer Reviewed
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[Journal Article] INO80 complex is required for damage-induced recombination2007
Author(s)
Kawashima, S., Ogiwara, H., Tada, S., Harata, M., Wintersberger, U., Enomoto, T., Seki, M
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Journal Title
Biochem. Biophys. Res. Commun 355
Pages: 835-841
Peer Reviewed
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[Journal Article] Ames test-negative carcinogen, ortho-phenyl phenol, binds tubulin and causes aneuploidy in budding yeast2007
Author(s)
Nunoshiba, T., Watanabe, E., Takahashi, T., Daigaku., Y., Ishikawa, S., Mochizuki, M., Ui, A., Enomoto, T., Yamamoto, K
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Journal Title
Mut. Res 617
Pages: 90-97
Peer Reviewed
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[Journal Article] Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells2007
Author(s)
Tomizawa, Y., Ui, A., Onoda, F., Ogiwara, H., Tada, S., Enomoto, T., Seki, M
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Journal Title
Genes Genet. Syst 82
Pages: 157-160
Peer Reviewed
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[Journal Article] Possible involvement of RecQL4 in the repair of double-strand DNA breaks in Xenopus egg extracts2007
Author(s)
Kumata, Y., Tada, S., Yamanada, Y., Tsuyama, T., Kobayashi, T., Dong, Y. -P., Ikegami, K., Murofushi, H., Seki, M., Enomoto, T
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Journal Title
Biochim. Biophys. Acta 1773
Pages: 556-564
Peer Reviewed
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[Journal Article] Chromatin loading of Smc5/6 is induced by DNA replication but not by DNA double-strand breaks2006
Author(s)
Tsuyama, T., Inou, K., Seki, M., Seki, T., Kumata, Y., Kobayashi, T., Kimura, K., Hanaoka, F., Enomoto, T., Tada, S
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Journal Title
Biochem. Biophys. Res. Commun 351
Pages: 935-939
Peer Reviewed
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[Journal Article] A novel Rad18 function involved in the protection of the vertebrate genome after exposure to camptothecin2006
Author(s)
Yoshimura, A., Nishino, K., Takezawa, J., Tada, S., Kobayashi, T., Sonoda, E., Kawamoto, T., Takeda, S., Ishii, Y., Yamada, K., Enomoto, T., Seki, M
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Journal Title
DNA Repair 5
Pages: 1307-1316
Peer Reviewed
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[Journal Article] Ubc9 and Mms21 mediated sumoylation counteracts recombinogenic events at damaged replication forks2006
Author(s)
Branzei, D., Sollier, J., Liberi, G., Zhao, X, Maeda, D., Seki, M., Enomoto, T., Ohta, K., Foiani, M
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Journal Title
Peer Reviewed
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[Journal Article] BLM is an early responder to DNA double-strand breaks. Biochem. Biophys2006
Author(s)
Karmakar, P., Seki, M., Kanamori, M., Hashiguchi, K., Ohtsuki, M., Murata, E., Inoue, E., Tada, S., Lan, L., Yasui, A., Enomoto, T
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Journal Title
Res. Commun 348
Pages: 62-69
Peer Reviewed
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[Journal Article] Loss of heterozygosity in yeast can occur by ultraviolet irradiation during the S phase of the cell cycle2006
Author(s)
Daigaku, Y., Mashiko, S., Mishiba, K., Yamamura, S., Ui, A., Enomoto, T., Yamamoto, K
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Journal Title
Mutat. Res 600
Pages: 177-183
Peer Reviewed
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[Journal Article] Bloom helicase and DNA topoisomerase III・are involved in the dissolution of sister chromatids2006
Author(s)
Seki, M., Nakagawa, T., Seki, T., Kato, G., Tada, T., Takahashi, Y., Yoshimura, A., Kobayashi, T., Aoki, A., Otsuki, M., Habermann, F.A., Tanabe, H., Ishii, Y., Enomoto, T
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Journal Title
Mol. Cell. Biol 26
Pages: 6299-6307
Peer Reviewed
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[Journal Article] Dpb11, the budding yeast homologue of TopBP1, functions with the checkpoint clamp in recombination repair2006
Author(s)
Ogiwara, H., Ui, A., Onoda, F., Tada, S., Enomoto, T., Seki, M
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Journal Title
Nuc. Acids Res 34
Pages: 3389-3398
Peer Reviewed
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[Journal Article] Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development2005
Author(s)
Yanagi, K., Mizuno, T., Tsuyama, T., Tada, S., Iida, Y., Sugimoto, A., Eki, T., Enomoto, T., Hanaoka F
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Journal Title
J. Biol. Chem 280
Pages: 19689-19694
Peer Reviewed
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[Journal Article] Functional relationships of FANCC to homologous recombination, translesion synthesis, and BLM2005
Author(s)
Hirano, S., Yamamoto, K., Ishiai, M., Yamazoe, M., Seki, M., Matsushita, N., Ohzeki, M., Yamashita, Y.M., Arakawa, H., Buerstedde, J. -M., Enomoto, T., Takeda, S., Thompson, L.H., Takata, M
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Journal Title
Peer Reviewed
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[Journal Article] DNA topoisomerase III is essential for damage-induced recombination2005
Author(s)
Ui, A., Seki, M., Ogiwara, H., Onodera, R., Fukushige, S., Onoda, F., Enomoto, T
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Journal Title
DNA Repair 4
Pages: 191-201
Peer Reviewed
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