Development of new gene therapy vectors and the preclinical cancer animal model system using common marmoset

2009 Fiscal Year Final Research Report

• Project/Area Number: 17016053
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University
• Principal Investigator: TANI Kenzaburo Kyushu University, 生体防御医学研究所, 教授 (00183864)
• Co-Investigator(Kenkyū-buntansha): NAKAZAKI Yukou 九州大学, 生体防御医学研究所, 助手 (00346850) ; HISANO Terumasa 九州大学病院, 助手 (40380399) ; UCHIMARU Kaoru 東京大学, 医科学研究所, 助教授 (60251203) ; MIURA Ryuichi 東京大学, 医科学研究所 (00322074) ; NAKAYAMA Masaharu 九州大学病院, 助教 (80363349) ; KURITA Ryo 九州大学, 生体防御医学研究所, 助教 (90380526) ; SATO Hiroki 東京大学, 医科学研究所, 助教 (50418654)
• Co-Investigator(Renkei-kenkyūsha): UCHIMARU Kaoru 東京大学, 医科学研究所, 准教授 (60251203) ; SATO Hiroki 東京大学, 医科学研究所, 助教 (50418654)
• Project Period (FY): 2005 – 2009
• Keywords: コモンマーモセット / フィラデルフィア染色体陽性急性リンパ性白血病 / 顆粒系マクロファージコロニー刺激因子 / 免疫遺伝子治療 / ケモカイン / 麻疹ウイルス / エンテロウイルス

Research Abstract

To develop new therapies for cancer, we have been involved in the development of new animal cancer models and new gene therapy methods. During the past 5 years, we could successfully develop human T-lymphotropic virus (HTLV)-1 carrier model in small monkey of common marmoset. This animal model will become very helpful to elucidate pathophysiology of human adult T-cell leukemia and to develop its new therapeutic modality. We also developed new oncolytic virus gene therapy methods using measles virus and enterovirus, and new immune gene therapy method using GM-CSF expressing carrier cells. We are now preparing the phase I clinical study using this new gene therapy method.

Research Products

• [Journal Article] Enhanced antitumor effects of an oncolytic measles virus vaccine strain expressing the wild-type N, P, L genes on human renal cell carcinoma (2010)
  • Author(s): Xin M, Nakamura T,, Tani, K., et. al.
  • Journal Title: Mol Ther. 18(3) Pages: 544-51
• [Journal Article] Curiel DT. A genetically engineered adenovirus vector targeted to CD40 mediates transduction of canine dendritic cells and promotes antigen-specific immune responses in vivo (2009)
  • Author(s): Thacker EE, Nakayama M, Tani K, et. al.
  • Journal Title: Vaccine 27 Pages: 7116-7124
• [Journal Article] Developmentof monoclonal antibodies for analyzing immune and hematopoietic systems of common marmoset (2009)
  • Author(s): Kametani, Y., Suzuki, D., Tani, K., et. al.
  • Journal Title: Exp Hematol 37 Pages: 1318-1329
• [Journal Article] Non-transmissible SeV encoding GM-CSF is a novel and potent vector system to produce autologous tumor vaccines (2008)
  • Author(s): Inoue, H., Iga, H., Tani, K., et. al
  • Journal Title: Cancer Science 99 Pages: 2315-2326
• [Journal Article] TARC and RANTES enhance antitumor immunity induced by the GM-CSF-transduced tumor vaccine in a mouse tumor model (2008)
  • Author(s): Inoue, H., Iga, M., Tani, K., et. al.
  • Journal Title: Cancer Immunol Immun 57 Pages: 1399-1411
• [Journal Article] A Critical role of CD30 ligand/CD30 in controlling inflammatory bowel diseases in mice (2007)
  • Author(s): Sun, X, m Somada, S., Tani, K., et. al.,, Podack, E., Yoshikai, Y.
  • Journal Title: Gastroenterology 134(2) Pages: 447-458
• [Journal Article] Inhibition of tumor growth through suppression of angiogenesis by brain-specific angiogenesis inhibitor 1 gene transfer in murine renal cell carcinoma (2007)
  • Author(s): Kudo S, Konda R, Tani K, et. al., T.
  • Journal Title: Oncol Rep 18 Pages: 785-791
• [Journal Article] Modification of glucocorticoid sensitivity by MAP kinase signaling pathways in glucocorticoid-induced T-cell apoptosis (2006)
  • Author(s): Tanaka, T., Okabe, T., Tani, K., et. al.
  • Journal Title: Exp Hematol 34 Pages: 1542-1552
• [Journal Article] Meeting Report: Nonhuman Primates: Embryonic stem cells and transgenesis (2006)
  • Author(s): Horn, P.A., Tani, K., et. al.
  • Journal Title: Cloning and Stem Cells 8 Pages: 124-129
• [Journal Article] Fhit modulates the DNA damage checkpoint response (2006)
  • Author(s): Ishii, H., Mimori, K., Tani, K., et. al.
  • Journal Title: Cancer Res 66 Pages: 11287-11292
• [Journal Article] Tal1/scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) ES cells (2006)
  • Author(s): Kurita, R., Sasaki, E., Tani, K., et. al.
  • Journal Title: Stem Cells 24 Pages: 2014-2022
• [Journal Article] Serial analysis of gene expression in progressing and regressing mouse tumors implicates the involvement of RANTES and TARC in antitumor immune responses (2006)
  • Author(s): Nakazaki, Y., Hase, H., Tani, K., et. al.
  • Journal Title: Mol Ther 14 Pages: 599-606
• [Journal Article] a novel p53 homologue, potentiates p53 activity and is a human cancer gene therapy candidate (2006)
  • Author(s): Kunisaki, R., Ikawa, S., Tani, K., et. al.
  • Journal Title: J Gene Med. 8 Pages: 1121-1130
• [Journal Article] An adenovirus serotype 5 vector with fibers derived from ovine atadenovirus demonstrates CAR-independent tropism and unique biodistribution inmice (2006)
  • Author(s): Nakayama M, Both GW, Tani, K., et. al.
  • Journal Title: Virology 350 Pages: 103-115
• [Journal Article] Rb plays a role in survival of Abl-dependent human tumor cells as a downstream effector of Abl tyrosine kinase (2006)
  • Author(s): Nagano, K., Itagaki, C., Tani, K., et. al.
  • Journal Title: Oncogene 25 Pages: 493-502
• [Journal Article] Antiangiogenic activity of BAI1 in vivo:implications for gene therapy of human glioblastomas (2006)
  • Author(s): Kang X., Xinru Xiao, Tani, K., et. al.
  • Journal Title: Cancer Gene Ther 13 Pages: 385-392
• [Journal Article] A novel role of CD30/CD30L signaling in the generation of long-lived memory CD8+T cells (2005)
  • Author(s): Nishimura H., Yajima T., Tani K., et. al.
  • Journal Title: J. Immunol 175 Pages: 4627-4634
• [Journal Article] Establishment of novel embryonic stem cell lines derived from the common marmoset (2005)
  • Author(s): Sasaki, E., Hanazawa, K., Tani, K.
  • Journal Title: Stem cells 23 Pages: 1304-1313
• [Presentation] Engineered measles virus MV-NPL as a novel oncolytic therapy (2009)
  • Author(s): Okazaki, T., Meng, X., Tani. K., et al
  • Organizer: 第15回日本遺伝子治療学会
  • Place of Presentation: 大阪
  • Year and Date: 20090709-20090711
• [Presentation] MV-NPL, as a new therapeutic oncolytic measles virus candidate (2009)
  • Author(s): Okazaki, T., Meng, T., Tani., T., et al.
  • Organizer: 12th Annual Meeting of the American Society of Gene Therapy
  • Year and Date: 20090527-20090530
• [Presentation] 進行・再発固形腫瘍(消化器癌・肺癌)に対するCyclophosphamide(CY)併用新規腫瘍関連抗原由エピトープペプチドカクテルを用いた腫瘍特異的強化ワクチン療法の検討(第I相臨床試験) (2009)
  • Author(s): 土方康基、伊賀睦了、谷憲三朗, 他
  • Organizer: 第106回日本内科学会総会
  • Place of Presentation: 東京
  • Year and Date: 20090410-20090412
• [Presentation] In vitro induction of the hematopoietic progenitor/stem cells from human ES cells (2009)
  • Author(s): Kurita, R., Kageyama, R., Tani. K.
  • Organizer: ASGCT 12th annual meeting
  • Place of Presentation: San Diego
  • Year and Date: 20090000
• [Presentation] Efficient Induction of Hematopoietic Progenitor/Stem Cell Differentiation by Lentiviral Gene Transduction of TAL1/SCL into Human ES Cells (2009)
  • Author(s): Miura, Y., Kurita, R., Tani. K.
  • Organizer: 51st ASH Annual Meeting and Exposition
  • Place of Presentation: New Orleans
  • Year and Date: 20090000
• [Presentation] Expression Cloning of Genes Enabling Erythropoietin-Independent Erythropoiesis in Vitro (2009)
  • Author(s): Yokoo, T., Kurita, R., Tani. K.
  • Organizer: 51th ASH (American Society of Hematology) Annual Meeting and Exposition
  • Place of Presentation: New Orleans, LA
  • Year and Date: 20090000
• [Presentation] Blocking LT4 signaling maintains the in vivo antitumor effects of GM-CSF transduced tumor cells (2008)
  • Author(s): Nabeta, H., Inoue, H., Tani, K., et al.
  • Organizer: Japan Society of Gene Therapy (JSGT) 14th Annual Meeting
  • Place of Presentation: Sapporo
  • Year and Date: 2008-06-13
• [Presentation] Gene Therapy in USA. Educational Seminar III. (2008)
  • Author(s): Tani, K.
  • Organizer: Japan Society of Gene Therapy (JSGT) 14th Annual Meeting
  • Place of Presentation: Sapporo
  • Year and Date: 2008-06-13
• [Presentation] Enhanced antitumor immunity by adoptively transfused Sendai virus mediated GM-CSF transduced dendritic cells in LLC bearing mice (2008)
  • Author(s): Inoue, H., Iga, M., Tani, K., et al.
  • Organizer: Japan Society of Gene Therapy (JSGT) 14th Annual Meeting
  • Place of Presentation: Sapporo
  • Year and Date: 2008-06-12
• [Presentation] Blocking LT4 signaling maintains the antitumor effect of GM-CSF in the tumor challenge model using BLT-1-/- mice (2008)
  • Author(s): Nabeta, H., Inoue, H., Tani, K., et al.
  • Organizer: America Society of Gene Therapy (ASGT) 11h Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-05-29
• [Book] 分子細胞治療(悪性腫瘍に対する遺伝子治療, 8(6)) (2009)
  • Author(s): 谷憲三朗
  • Total Pages: 425-435
• [Book] 最新医学(コモンマーモセット, 64) (2009)
  • Author(s): 谷憲三朗
  • Total Pages: 723-737
• [Book] 日本医師会雑誌(遺伝子治療, 138) (2009)
  • Author(s): 谷憲三朗
  • Total Pages: S144-146
• [Patent(Industrial Property Rights)] NOVEL ONCOLYTIC VIRUS FOR LUNG CANCER (2010)
  • Inventor(s): 谷憲三朗
  • Industrial Property Number: 61/315129
  • Filing Date: 20100226,20100318
• [Patent(Industrial Property Rights)] THERAPEUTIC AGENT FOR MALIGNANT TUMOR USING ONCOLYTIC MEASLES VIRUS, AND THERAPEUTIC METHOD USING THE AGENT
  • Inventor(s): 谷憲三朗
  • Industrial Property Number: 特許・61/308477