2009 Fiscal Year Final Research Report
Selective targeted drug and gene delivery for cancer treatment
| Project/Area Number |
17016059
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
HAMADA Hirohumi Sapporo Medical University, 医学部, 教授 (00189614)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Kazunori 札幌医科大学, 医学部, 准教授 (60233780)
NAKAMURA Kiminori 札幌医科大学, 医学部, 講師 (80381276)
HAMA Susumu 札幌医科大学, 医学部, 助教 (60438041)
YAMAGUCHI Miki 札幌医科大学, 医学部, 助教 (10530454)
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| Project Period (FY) |
2005 – 2009
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| Keywords | 遺伝子治療 / アデノウィルスベクター / モノクローナル抗体 / 標的化抗体 / 標的化遺伝子導入 / 膵癌 / 悪性黒色腫 / 前立腺癌 |
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| Research Abstract |
In order to systematically search for the target molecules for cancer gene therapy, we generated a fiber-modified recombinant adenovirus, Adv-FZ33, which contains a Z33 domain with a high affinity for immunoglobulin Fc. By screening with Adv-FZ33, we established a large number of high affinity targeting antibodies (Ab), and identified their target antigen (Ag) molecules, e.g., a panel of 60 Ag species. During these efforts, we also established a unique method (EZiTox) to generate immunotoxins (iTox), which consists of the targeting Ab conjugated with ribosome-inactivating enzyme toxins. EZiTox method will be a strong screening method to find a powerful combination of Ag/Ab for cancer iTox-chemotherapy.
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[Journal Article] Selective gene transfer into neurons via Na, K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector.2008
Author(s)
Ishii K, Nakamura K, Kawaguchi S, Li R, Hirai S, Sakuragi N, Wada T, Kato K, Yamashita T, Hamada H
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Journal Title
J. Gene Med. 10(6)
Pages: 597-609
Peer Reviewed
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[Journal Article] E1A, E1B double-restricted adenovirus with RGD-fiber modification exhibits enhanced oncolysis for CAR-deficient biliary cancers.2007
Author(s)
Wakayama M, Abei M, Kawashima R, Seo E, Fukuda K, Uqai H, Murata T, Tanaka N, Hyodo I, Hamada H, Yokoyama K
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Journal Title
Clin. Cancer Res. 13(10)
Pages: 3043-3050
Peer Reviewed
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[Journal Article] p53-induced inhibition of Hif-1 causes cardiac dysfunction during pressure overload.2007
Author(s)
Sano M, Minamino T, Toko H, Miyauchi H, Orimo M, Qin Y, Akazawa H, Tateno K, Kayama Y, Harada M, Shimizu I, Asahara T, Hamada H, Tomita S, Molkentin JD, Zou Y, Komuro I
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Journal Title
Nature 446(7134)
Pages: 444-448
Peer Reviewed
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[Journal Article] Effective gene therapy of biliary tract cancers by a conditionally replicative adenovirus expressing uracil phosphoribosyltransferase: significance of timing of 5-fluorouracil administration.2005
Author(s)
Seo E, Abei M, Wakayama M, Fukuda K, Ugai H, Murata T, Todoroki T, Matsuzaki Y, Tanaka N, Hamada H, Yokoyama K
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Journal Title
Cancer Res. 65(2)
Pages: 546-552
Peer Reviewed
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[Journal Article] Bcl-xL gene transfer inhibits Bax translocation and prolongs cardiac cold preservation time in rat.2005
Author(s)
Huang J, Nakamura K, Ito Y, Uzuka T, Morikawa M, Hirai S, Tomihara K, Tanaka T, Masuta Y, Ishii K, Kato K, Hamada H
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Journal Title
Circulation 112(1)
Pages: 76-83
Peer Reviewed
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[Journal Article] Mesenchymal stem cells that produce neurotrophic factors reduce ischemic damage in the rat middle cerebral artery occlusion model.2005
Author(s)
Kurozumi K, Nakamura K, Tamiya T, Kawano Y, Ishii K, Kobune M, Hirai S, Uchida H, Sasaki K, Ito Y, Kato K, Honmou O, Houkin K, Date I, Hamada H
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Journal Title
Mol. Ther. 11(1)
Pages: 96-104
Peer Reviewed
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