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2007 Fiscal Year Final Research Report

Atelocollagen-mediated delivery of nucleic acid medicines

Research Project

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Project/Area Number 17016086
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East

Principal Investigator

OCHIYA Takahiro  National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East, 研究所がん転移研究室, 独立室長 (60192530)

Co-Investigator(Kenkyū-buntansha) 竹下 文隆  国立がんセンター(研究所及び東病院臨床開発センター), 研究所がん転移研究室 (40466199)
石川 哲也  国立がんセンター(研究所及び東病院臨床開発センター), 研究所がん転移研究室 (90398743)
Project Period (FY) 2005 – 2009
Keywordsアテロコラーゲン / デリバリー / 核酸医薬 / siRNA / miRNA / がん / 転移
Research Abstract

RNA interference (RNAi) is a relatively a novel phenomenon of posttranscriptional gene silencing to regulate the expression of multiple genes involved in a wide range of biological processes. The gene-silencing technology via RNAi has also been developed into a commonly anti-cancer method. Furthermore, in vivo data indicate that small interfering RNAs (siRNAs and miRNAs) may be used to treat human diseases. However, the most challenging issue to a successful in vivo application is the development of a delivery system that can transport RNAi molecules into the tissues and/or the cells of interest. Also, the evaluation of RNAi potency in vivo is central for the selection of therapeutic siRNAs and miRNAs. In this study, the effects of atelocollagen-delivered siRNAs and miRNAs in live animals were monitored using bioluminescence imaging. Our study indicates the therapeutic potential of siRNA and miRNA in an animal model of cancer metastasis with systemic siRNA and miRNA injection and suggest that systemic delivery of RNAi could be used to treat advanced prostate cancer.

  • Research Products

    (46 results)

All 2010 2009 2008 2007 Other

All Journal Article (17 results) (of which Peer Reviewed: 1 results) Presentation (26 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Systemic delivery of synthetic microRNA-16 inhibits the growth of metastatic prostate tumors via downregulation of multiple cell-cycle genes.2010

    • Author(s)
      Takeshita F, Patrawala L, Osaki M, Takahashi R, Yamamoto Y, Kosaka N, Kawamata M, Kelnar K, Bader AG, Brown D, Ochiya T.
    • Journal Title

      Mol Ther 18

      Pages: 181-187

  • [Journal Article] Mutant mouse p53 transgene elevates the chemical induction of tumors that respond to gene silencing with siRNA.2010

    • Author(s)
      anooka H, Tatsumi K, Tsuji H, Noda Y, Katsube T, Ishii H, Ootsuyama A, Takeshita F, Ochiya T.
    • Journal Title

      Cancer Gene Ther 17

      Pages: 1-10

  • [Journal Article] Screening of potential molecular targets for colorectal cancer therapy.2009

    • Author(s)
      Honma K, Takemasa I, Matoba R, Yamamoto Y, Takeshita F, Mori M, Monden M, Matsubara K, Ochiya T.
    • Journal Title

      Int J Gen Med 2

      Pages: 243-257

    • Peer Reviewed
  • [Journal Article] MicroRNA-500 as a potential diagnostic marker for hepatocellular carcinoma.2009

    • Author(s)
      Yamamoto Y, Kosaka N, Tanaka M, Koizumi F, Kanai Y, Mizutani T, Murakami Y, Kuroda M, Miyajima A, Kato T, Ochiya T.
    • Journal Title

      Biomarkers 14

      Pages: 529-538

  • [Journal Article] Dicer is required for maintaining adult pancreas.2009

    • Author(s)
      Morita S, Hara A, Kojima I, Horii T, Kimura M, Kitamura T, Ochiya T, Nakanishi K, Matoba R, Matsubara K, Hatada I.
    • Journal Title

      PLoS ONE 4

      Pages: e4212

  • [Journal Article] An siRNA against JC virus (JCV) agnoprotein inhibits JCV infection in JCV-producing cells inoculated in nude mice.2008

    • Author(s)
      Matoba T, Orba Y, Suzuki T, Makino Y, Shichinohe H, Kuroda S, Ochiya T, Itoh H, Tanaka S, Nagashima K, Sawa H.
    • Journal Title

      Neuropathology. 28

      Pages: 286-294

  • [Journal Article] RNAi-based drug discovery and its application to therapeutics.2008

    • Author(s)
      Hokaiwado N, Takeshita F, Banas A, Ochiya T.
    • Journal Title

      IDrugs 11

      Pages: 274-278

  • [Journal Article] RPN2 gene confers docetaxel resistance in breast cancer.2008

    • Author(s)
      Honma K, Iwao-Koizumi K, Takeshita F, Yamamoto Y, Yoshida T, Nishio K, Nagahara S, Kato K, Ochiya T.
    • Journal Title

      Nat Med 14

      Pages: 939-948

  • [Journal Article] Presentation of functional foreign peptides on the surface of SV40 virus-like particles.2008

    • Author(s)
      Takahashi R, Kanesashi S, Inoue T, Enomoto T, Kawano M, Tsukamoto H, Takeshita F, Imai T, Ochiya T, Kataoka K, Yamaguchi Y, Handa H.
    • Journal Title

      J Biotechnol 135

      Pages: 385-392

  • [Journal Article] Antitumor effect of SN-38-releasing polymeric micelles, NK012, on spontaneous peritoneal metastases from orthotopic gastric cancer in mice compared with irinotecan.2008

    • Author(s)
      akajima TE, Yanagihara K, Takigahira M, Yasunaga M, Kato K, Hamaguchi T, Yamada Y, Shimada Y, Mihara K, Ochiya T, Matsumura Y.
    • Journal Title

      Cancer Res 68

      Pages: 9318-9322

  • [Journal Article] Optical imaging of RNAi-mediated silencing of cancer.2008

    • Author(s)
      Ochiya T, Honma K, Takeshita F, Nagahara S.
    • Journal Title

      Proc of SPIE 6868

  • [Journal Article] Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.2008

    • Author(s)
      Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R.
    • Journal Title

      Cancer Sci 99

      Pages: 810-815

  • [Journal Article] Glutathione S-transferase Pi mediates proliferation of andorogen-independent prostate cancer cells.2008

    • Author(s)
      Hokaiwado N, Takeshita F, Naiki-Ito A, Asamoto M, Ochiya T, Shirai T.
    • Journal Title

      Carcinogenesis 29

      Pages: 1134-1138

  • [Journal Article] MicroRNAs as biomarkers and therapeutic drugs in human cancer.2008

    • Author(s)
      Osaki M, Takeshita F, Ochiya T.
    • Journal Title

      Biomarkers 13

      Pages: 658-670

  • [Journal Article] Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation.2008

    • Author(s)
      Kosaka N, Sugiura K, Yamamoto Y, Yoshioka Y, Miyazaki H, Komatsu N, Ochiya T, Kato T.
    • Journal Title

      Br J Haematol 142

      Pages: 293-300

  • [Journal Article] Atelocollagen-mediated Drug Discovery Technology.2007

    • Author(s)
      Ochiya T, Honma K, Takeshita F, Nagahara S.
    • Journal Title

      Expert Opin. Drug Discov. 2

      Pages: 159-167

  • [Journal Article] Type I collagen gene suppresses tumor growth and invasion of malignant human glioma cells.2007

    • Author(s)
      Honma K, Miyata T, Ochiya T.
    • Journal Title

      Cancer Cell Int 7

      Pages: 12

  • [Presentation] microRNAによる Cancer Stem Cell Therapy の可能性2009

    • Author(s)
      落谷孝広
    • Organizer
      第32回日本分子生物学会(/講演)
    • Place of Presentation
      横浜
    • Year and Date
      20091209-20091212
  • [Presentation] RPN2による糖鎖修飾を介した薬剤耐性、浸潤転移の制御機構2009

    • Author(s)
      落谷孝広
    • Organizer
      第82回日本生化学会学会(/講演)
    • Place of Presentation
      神戸
    • Year and Date
      20091021-20091025
  • [Presentation] マイクロRNAによるがんの診断と治療2009

    • Author(s)
      落谷孝広
    • Organizer
      第68回日本癌学会学術総会(がんにおけるmicroRNA制御異常シンポジウム/講演)
    • Place of Presentation
      横浜
    • Year and Date
      20091001-20091003
  • [Presentation] RPN2 as a novel therapeutic target for cancer drug resistance.2009

    • Author(s)
      Ochiya T.
    • Organizer
      9th Jenner Glycobiology and Medicine Symposium
    • Place of Presentation
      Brussels, Belgium.
    • Year and Date
      20090912-20090918
  • [Presentation] microRNAによるがんの診断治療2009

    • Author(s)
      落谷孝広
    • Organizer
      第1回日本RNAi研究会
    • Place of Presentation
      広島大学
    • Year and Date
      20090828-20090829
  • [Presentation] CRS (Controlled Release Society).2009

    • Author(s)
      Ochiya T, Takeshita F.
    • Organizer
      Oligonucleotides delivery 36THANNUAL MEETING AND EXPOSITION OF THE CONTROLLED RELEASE SOCIETY
    • Place of Presentation
      Copenhagen, Denmark.
    • Year and Date
      20090716-20090724
  • [Presentation] 核酸デリバリーが拓くnon-coding small RNAによる疾患解明2009

    • Author(s)
      落谷孝広
    • Organizer
      遺伝子・デリバリー研究会第9回シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      20090709-20090711
  • [Presentation] micro RNA as a Novel Modality for Cancer Therapy2009

    • Author(s)
      落谷孝広
    • Organizer
      第2回DKFZ-NCC Workshop on Cancer Research Tokyo
    • Place of Presentation
      がんセンター研究所
    • Year and Date
      20090707-20090709
  • [Presentation] Therapeutic potential of microRNA against cancer.2009

    • Author(s)
      Ochiya T, Takeshita F.
    • Organizer
      MicroRNAi-meeting.
    • Place of Presentation
      RNAi World Congress Boston, USA.
    • Year and Date
      20090512-20090513
  • [Presentation] RNAi-based oligonucleotides therapy2009

    • Author(s)
      落谷孝広
    • Organizer
      Kashiwa Symposium on Cancer Biology 2009
    • Place of Presentation
      がんセンター東病院
    • Year and Date
      2009-11-13
  • [Presentation] ヒト乳がん細胞株における薬剤抵抗性に関与するmiRNAの同定.2008

    • Author(s)
      高橋陵宇, 竹下文隆, 山本雄介, 箕浦加穂, 田谷敏貴, 小坂展慶, 落谷孝広.
    • Organizer
      第31回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-12
  • [Presentation] ヒト骨肉腫細胞における肺転移関連マイクロRNAの検出.2008

    • Author(s)
      尾崎充彦, 竹下文隆, 小坂展慶, 井藤久雄, 押村光雄, 落谷孝広.
    • Organizer
      第31回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      20081209-12
  • [Presentation] Therapeutic potential of microRNA against cancer.2008

    • Author(s)
      Ochiya T.
    • Organizer
      The Right RNAi Meeting
    • Place of Presentation
      Brussels, Belgium
    • Year and Date
      20081102-09
  • [Presentation] RNAi-mediated silencing of cancer.2008

    • Author(s)
      Takahiro Ochiya.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-30
  • [Presentation] Identification of MicroRNAs Involved in Drug Resistance in Human Breast Cancer Cell Lines.2008

    • Author(s)
      Fumitaka Takeshita, Yusuke Yamamoto, Kaho Minoura, Ryou-u Takahashi, Nobuyuki Kosaka, Kimi Honma, Takahiro Ochiya.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-20081030
  • [Presentation] Detection of lung meastasis-related microRNA in human osteosarcoma cell.2008

    • Author(s)
      Mitsuhiko Osaki, Fumitaka Takeshita, Hisao Ito, Mitsuo Oshimura, Takahiro Ochiya.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-20081030
  • [Presentation] The inhibition of human prostate cancer cells on bone-metastatic site by treatment with miR-16.2008

    • Author(s)
      Ryou-u Takahashi, Fumitaka Takeshita, Takahiro Ochiya.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-20081030
  • [Presentation] Identification and characterization of a novel tumor suppressor gene on chromosome arm 18q in human pancreatic cancer.2008

    • Author(s)
      Satoru Yokoyama, Fuyuhiko Motoi, Hideo Ohtsuka, Masaharu Ishida, Nobukazu Tsukamoto, Naoyuki Kaneko, Shinichi Egawa, Michiaki Unno, Toru Furukawa, Makoto Sunamura, Takahiro Ochiya, Akira Horii.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-20081030
  • [Presentation] Development of a mouse model for suppression of peritoneal metastasis for diffuse-type gastric cancer.2008

    • Author(s)
      Takeshi Fujita, Fumitaka Takeshita, Kazuyoshi Yanagihara, Hiroyuki Ohta, Tomoko Mabuchi, Kazuhiko Aoyagi, Takeo Fukagawa, Hitoshi Katai, Takeshi Sano, Takahiro Ochiya, Teruhiko Yoshida, Hiroki Sasaki.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-20081030
  • [Presentation] Potential of miRNA as cancer diagnosis and a target for therapy.2008

    • Author(s)
      Takahiro Ochiya.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-20081030
  • [Presentation] Highly efficient microRNA delivery to tumor metastasis.2008

    • Author(s)
      Takahiro Ochiya.
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      20081028-20081030
  • [Presentation] 幹細胞の持つ肝細胞分化能と肝疾患治療RNAiによるがんの予防・診断・治療(シンポジウム)2008

    • Author(s)
      落谷孝広
    • Organizer
      第36回薬物活性シンポジウム
    • Place of Presentation
      徳島
    • Year and Date
      20081023-20081025
  • [Presentation] MicroRNAs AS THERAPEUTIC TARGETS:POTENTIAL EFFECT ON PROSTATE CANCER MANAGEMENT.2008

    • Author(s)
      Takahiro Ochiya
    • Organizer
      第14回日本遺伝子治療学会総会
    • Place of Presentation
      札幌
    • Year and Date
      20080613-20080615
  • [Presentation] RNAi-based therapeutics against cancer.2008

    • Author(s)
      Ochiya T.
    • Organizer
      RNAi World Congress 2008
    • Place of Presentation
      Boston, USA
    • Year and Date
      20080000
  • [Presentation] Optical imaging of RNAi-mediated silencing of cancer.2008

    • Author(s)
      Ochiya T, Honma K, takeshita F, Nagahara S.
    • Organizer
      Progress in Biomedical Optics and Imaging - SPIE 2008
    • Place of Presentation
      San Jose, CA, USA
    • Year and Date
      20080000
  • [Presentation] Global expression profiling of miRNA in liver development.

    • Author(s)
      Yamamoto Y, Ochiya T.
    • Organizer
      FASEB Summer Research Conference
    • Place of Presentation
      Colorado, USA
  • [Book] siRNA and miRNA Gene Silencing.(Sioud M (ed), Local and systemic delivery of siRNAs for oligonucleotide therapy.)2009

    • Author(s)
      Takeshita F, Hokaiwado N, Honma K, Banas A, Ochiya T.
    • Total Pages
      83-92
    • Publisher
      Humana Press
  • [Remarks]

    • URL

      http://www.ncc.go.jp/jp/nccri/divisions/15meta/15meta.html

  • [Patent(Industrial Property Rights)] 核酸導入を促進させる方法2008

    • Inventor(s)
      落谷孝広、寺田雅昭、阿蘇雄
    • Industrial Property Rights Holder
      大日本住友製薬株式会社、株式会社高研
    • Industrial Property Number
      新規発明特許・特許第4081436号
    • Filing Date
      2008-02-15
    • Acquisition Date
      2008-02-15

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Published: 2011-06-18   Modified: 2016-04-21  

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