2007 Fiscal Year Final Research Report
Atelocollagen-mediated delivery of nucleic acid medicines
| Project/Area Number |
17016086
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East |
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Principal Investigator |
OCHIYA Takahiro National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East, 研究所がん転移研究室, 独立室長 (60192530)
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| Co-Investigator(Kenkyū-buntansha) |
竹下 文隆 国立がんセンター(研究所及び東病院臨床開発センター), 研究所がん転移研究室 (40466199)
石川 哲也 国立がんセンター(研究所及び東病院臨床開発センター), 研究所がん転移研究室 (90398743)
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| Project Period (FY) |
2005 – 2009
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| Keywords | アテロコラーゲン / デリバリー / 核酸医薬 / siRNA / miRNA / がん / 転移 |
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| Research Abstract |
RNA interference (RNAi) is a relatively a novel phenomenon of posttranscriptional gene silencing to regulate the expression of multiple genes involved in a wide range of biological processes. The gene-silencing technology via RNAi has also been developed into a commonly anti-cancer method. Furthermore, in vivo data indicate that small interfering RNAs (siRNAs and miRNAs) may be used to treat human diseases. However, the most challenging issue to a successful in vivo application is the development of a delivery system that can transport RNAi molecules into the tissues and/or the cells of interest. Also, the evaluation of RNAi potency in vivo is central for the selection of therapeutic siRNAs and miRNAs. In this study, the effects of atelocollagen-delivered siRNAs and miRNAs in live animals were monitored using bioluminescence imaging. Our study indicates the therapeutic potential of siRNA and miRNA in an animal model of cancer metastasis with systemic siRNA and miRNA injection and suggest that systemic delivery of RNAi could be used to treat advanced prostate cancer.
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Research Products
(46 results)
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[Journal Article] Systemic delivery of synthetic microRNA-16 inhibits the growth of metastatic prostate tumors via downregulation of multiple cell-cycle genes.2010
Author(s)
Takeshita F, Patrawala L, Osaki M, Takahashi R, Yamamoto Y, Kosaka N, Kawamata M, Kelnar K, Bader AG, Brown D, Ochiya T.
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Journal Title
Mol Ther 18
Pages: 181-187
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[Journal Article] MicroRNA-500 as a potential diagnostic marker for hepatocellular carcinoma.2009
Author(s)
Yamamoto Y, Kosaka N, Tanaka M, Koizumi F, Kanai Y, Mizutani T, Murakami Y, Kuroda M, Miyajima A, Kato T, Ochiya T.
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Journal Title
Biomarkers 14
Pages: 529-538
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[Journal Article] Dicer is required for maintaining adult pancreas.2009
Author(s)
Morita S, Hara A, Kojima I, Horii T, Kimura M, Kitamura T, Ochiya T, Nakanishi K, Matoba R, Matsubara K, Hatada I.
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Journal Title
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[Journal Article] An siRNA against JC virus (JCV) agnoprotein inhibits JCV infection in JCV-producing cells inoculated in nude mice.2008
Author(s)
Matoba T, Orba Y, Suzuki T, Makino Y, Shichinohe H, Kuroda S, Ochiya T, Itoh H, Tanaka S, Nagashima K, Sawa H.
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Journal Title
Neuropathology. 28
Pages: 286-294
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[Journal Article] Presentation of functional foreign peptides on the surface of SV40 virus-like particles.2008
Author(s)
Takahashi R, Kanesashi S, Inoue T, Enomoto T, Kawano M, Tsukamoto H, Takeshita F, Imai T, Ochiya T, Kataoka K, Yamaguchi Y, Handa H.
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Journal Title
J Biotechnol 135
Pages: 385-392
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[Journal Article] Antitumor effect of SN-38-releasing polymeric micelles, NK012, on spontaneous peritoneal metastases from orthotopic gastric cancer in mice compared with irinotecan.2008
Author(s)
akajima TE, Yanagihara K, Takigahira M, Yasunaga M, Kato K, Hamaguchi T, Yamada Y, Shimada Y, Mihara K, Ochiya T, Matsumura Y.
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Journal Title
Cancer Res 68
Pages: 9318-9322
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[Presentation] Identification and characterization of a novel tumor suppressor gene on chromosome arm 18q in human pancreatic cancer.2008
Author(s)
Satoru Yokoyama, Fuyuhiko Motoi, Hideo Ohtsuka, Masaharu Ishida, Nobukazu Tsukamoto, Naoyuki Kaneko, Shinichi Egawa, Michiaki Unno, Toru Furukawa, Makoto Sunamura, Takahiro Ochiya, Akira Horii.
Organizer
第67回日本癌学会学術総会
Place of Presentation
名古屋
Year and Date
20081028-20081030
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[Presentation] Development of a mouse model for suppression of peritoneal metastasis for diffuse-type gastric cancer.2008
Author(s)
Takeshi Fujita, Fumitaka Takeshita, Kazuyoshi Yanagihara, Hiroyuki Ohta, Tomoko Mabuchi, Kazuhiko Aoyagi, Takeo Fukagawa, Hitoshi Katai, Takeshi Sano, Takahiro Ochiya, Teruhiko Yoshida, Hiroki Sasaki.
Organizer
第67回日本癌学会学術総会
Place of Presentation
名古屋
Year and Date
20081028-20081030
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