Comparative Analysis of the DECODE Systems in Hematopoiesis and Oxidative Stress Response

2009 Fiscal Year Final Research Report

• Project/Area Number: 17054028
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: IGARASHI Kazuhiko Tohoku University, 大学院・医学系研究科, 教授 (00250738)
• Co-Investigator(Kenkyū-buntansha): MUTO Akihiko 東北大学, 大学院・医学系研究科, 講師 (80343292)
• Project Period (FY): 2005 – 2009
• Keywords: Bach1 / Bach2 / 転写因子 / クロマチン / 発現制御

Research Abstract

DECODE complexes of Bach1, Bach2, and MafK were purified and characterized by using mass spectrometry analysis. Bach1 was found to interact with the tumor suppressor p53 and to inhibit cellular senescence induced by oxidative stress. Bach1 recruits histone deacetylase 1 to some of the p53 target genes and promotes histone deacetylation. In addition, a DECODE gene circuit for the class switch recombination of antibody genes has been identified.

Research Products

• [Journal Article] Bach1-dependent and -independent regulation of heme oxygenase-1 in keratinocytes. (2010)
  • Author(s): Okada, S., Muto, A., Ogawa, E., Nakanome, A., Katoh, Y., Ikawa, S., Aiba, S., Igarashi, K., Okuyama, R
  • Journal Title: J. Biol. Chem (in press)
  • Peer Reviewed
• [Journal Article] Characterization of the cancer chemopreventive NRF2-dependent gene battery in human keratinocytes: demonstration that the KEAP1-NRF2 pathway, and not the BACH1-NRF2 pathway, controls cytoprotection against electrophiles as well as redox-cycling compounds. (2009)
  • Author(s): MacLeod, A. K, McMahon, M., Plummer, S.M., Higgins, L.G., Penning, T.M., Igarashi, K., Hayes JD
  • Journal Title: Carcinogenesis 30 Pages: 1571-1580
  • Peer Reviewed
• [Journal Article] Crystal structure of the Bach1 BTB domain and its regulation of homodimerization. (2009)
  • Author(s): Ito, N., Watanabe-Matsui, M., Igarashi, K., Murayama, K
  • Journal Title: Genes Cells 14 Pages: 1365-2443
  • Peer Reviewed
• [Journal Article] Bach1 inhibits oxidative stress-induced cellular senescence by impeding p53 function on chromatin. (2008)
  • Author(s): Dohi, Y., Ikura, T., Hoshikawa, Y., Katoh, Y., Ota, K., Nakanome, A., Muto, A., Omura, S., Ohta, T., Ito, A., Yoshida, M., Noda, T., Igarashi, K
  • Journal Title: Nature Struct. Mol. Biol 15 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] Regulation of the plasma cell transcription factor Blimp-1 gene by Bach2 and Bcl6. (2008)
  • Author(s): Ochiai, K., Muto, A., Tanaka, H., Takahashi, S., Igarashi, K
  • Journal Title: Int. Immunol 20 Pages: 453-460
  • Peer Reviewed
• [Journal Article] Heme induces ubiquitination and degradation of the transcription factor Bach1. (2007)
  • Author(s): Zenke-Kawasaki, Y., Dohi, Y., Katoh, Y., Ikura, T., Ikura, M., Asahara, T., Tokunaga, F., Iwai, K., Igarashi, K
  • Journal Title: Mol. Cell. Biol 27 Pages: 6962-6971
  • Peer Reviewed
• [Journal Article] Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells. (2006)
  • Author(s): Ochiai, K., Katoh, Y., Ikura, T., Hoshikawa, Y., Noda, T., Karasuyama, H., Tashiro, S., Muto, A., Igarashi, K
  • Journal Title: J. Biol. Chem 281 Pages: 38226-38234
  • Peer Reviewed
• [Journal Article] Effects of genetic ablation of bach1 upon smooth muscle cell proliferation and atherosclerosis after cuff injury. (2005)
  • Author(s): Omura, S., Suzuki, H., Toyofuku, M., Ozono, R., Kohno, N., Igarashi, K
  • Journal Title: Genes Cells 10 Pages: 277-285
  • Peer Reviewed
• [Presentation] 核内メチル化反応を支えるタンパク質ネットワーク (2010)
  • Author(s): 五十嵐和彦
  • Organizer: 日本薬学会シンポジウム
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2010-03-29
• [Presentation] Cross-talk between Bach1, NF-kB, and IL-6 in epithelial-mesenchymal transition (2009)
  • Author(s): Brydun, A., Nakanome A., Matsumoto, M., Ota, K., Dohi, Y., Igarashi, K
  • Organizer: American Association for Cancer Reserch International Conference on Frontiers in Basic Cancer Research
  • Place of Presentation: Boston, USA
  • Year and Date: 20091008-20091011
• [Presentation] Regulation of cell proliferation by the heme-Bach1 pathway (2006)
  • Author(s): Kazuhiko Igarashi
  • Organizer: Gordon Research Conference on Tetrapyrroles, Chemistry and Biology of
  • Place of Presentation: Newport, RI, USA
  • Year and Date: 20060723-20060728
• [Presentation] Heme-mediated gene regulation in mammalian cells (2005)
  • Author(s): K. Igarashi, Y. Zenke, Y. Dohi
  • Organizer: Cold Spring Harbor Laboratory Meeting "Mechanisms of eukaryotic transcription"
  • Place of Presentation: マイアミ. USA
  • Year and Date: 20050831-20050904