2009 Fiscal Year Final Research Report
In vivo Reconstruction of Functional Protein Complexes by Using Nano-particles and its Application for Artificial Cell
| Project/Area Number |
17076008
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
YOSHIMURA Shige h. Kyoto University, 大学院・生命科学研究科, 准教授 (90346106)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEYASU Kunio 京都大学, 大学院・生命科学研究科, 教授 (40135695)
HIZUME Kohjiv 京都大学, 大学院・生命科学研究科, 助教 (10378846)
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| Project Period (FY) |
2005 – 2009
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| Keywords | ナノパーティクル / 核輸送 / 量子ドット / クロマチン / 核内骨格 / 染色体タンパク質 |
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| Research Abstract |
In this project, we have obtained the following results;
i) By combining in vitro nucleosome reconstitution procedure and atomic force microscopy, "30-nm-chromatin fiber" was successfully reconstituted.
ii) By utilizing fast-scanning atomic force microscopy, molecular mechanisms of various enzymatic functions and processes have been elucidated.
iii) We established the technique to microinject a fluorescent particle into a living cell and manipulate it by optical trap without damaging the cell.
iv) By attaching nuclear localization signal peptide to quantum dots, we established the method to deliver nano-particles into a living cell nucleus.
v) Structural and functional properties of nuclear scaffold proteins have been obtained by combining proteomics, bioinformatics, molecular biology and biochemistry.
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[Journal Article] Atomic Force Microscopy Dissects the Hierarchy of Genome Architectures in Eukaryote, Prokaryote, and Chloroplast.2007
Author(s)
R.L. Ohniwa, K. Morikawa, J. Kim, T. Kobori, K. Hizume, R. Matsumi, H. Atomi, T. Imanaka, T. Ohta, C. Wada, S.H. Yoshimura, K. Takeyasu
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Journal Title
Microsc. Microanal. 13(1)
Pages: 3-12
Peer Reviewed
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[Journal Article] Single-molecule detection of phosphorylation-induced plasticity changes during ezrin activation.2007
Author(s)
D. Liu, L. Ge, F. Wang, H. Takahashi, D. Wang, Z. Guo, S.H. Yoshimura, T. Ward, X. Ding, K. Takeyasu, X. Yao
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Journal Title
FEBS Lett. 581(18)
Pages: 3563-3571
Peer Reviewed
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[Journal Article] Transcriptional co-activator PC4, a chromatin-associated protein, induces chromatin condensation.2006
Author(s)
C. Das, K. Hizume, K. Batta, B.R.P. Kumar, S.S. Gadad, S. Ganguly, S. Lorain, A. Verreault, P.. P. Sadhale, K. Takeyasu, T.K. Kundu
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Journal Title
Mol. Cell. Biol. 26
Pages: 8303-8315
Peer Reviewed
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