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2009 Fiscal Year Final Research Report

Multi-scale manipulation-based analyses and application of the artificial chromosome-like characteristics of the Epstein-Barr virus-based episomal vectors

Research Project

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Project/Area Number 17076013
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

MAZDA Osam  Kyoto Prefectural University of Medicine, 医学研究科, 准教授 (00271164)

Co-Investigator(Kenkyū-buntansha) IMANISHI Jiro  京都府立医科大学, 医学研究科, 教授 (40112510)
FUSHIKI Shinnji  京都府立医科大学, 医学研究科, 教授 (80150572)
NAKAYA Takaaki  大阪大学, 微生物病研究所, 特任准教授 (80271633)
Project Period (FY) 2005 – 2009
Keywords幹細胞 / 細胞情報・動態 / 遺伝子発現 / 再生医学 / サイトカイン / アレルギー / 腫瘍免疫 / エピゲノム制御
Research Abstract

Gene transfection provides a fundamental technology to analyze, estimate and manipulate cellular and biomolecular functions. The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) gene and oriP sequence offer characteristics of an artificial chromosome, drastically improving transduction rate as well as expression level in mammalian cells. Using multiscale manipulation technologies, we analyzed intracellular localization, interaction, replication and maintenance of EBNA1/oriP in living cells, and applied them to regulation of gene expression as well as to regenerative medicine.

  • Research Products

    (40 results)

All 2009 2008 2007 2006 2005 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (12 results) Book (9 results)

  • [Journal Article] Comparison of anti-rheumatic effects of local RNAi-based Therapy in collagen induced arthritis rats using various cytokine genes as molecular targets.2009

    • Author(s)
      Inoue A, Takahashi KA, Mazda O, Arai Y, Saito M, Kishida T, Shin-Ya M, Morihara T, Tonomura H, Sakao K, Imanishi J, Kubo T.
    • Journal Title

      Mod Rheumatol 19

      Pages: 125-133

    • Peer Reviewed
  • [Journal Article] Interleukin-27 activates natural killer cells and suppresses NK-resistant head and neck squamous cell carcinoma through inducing antibody-dependent cellular cytotoxicity.2009

    • Author(s)
      Matsui M, Kishida T, Nakano H, Yoshimoto K, Shin-Ya M, Shimada T, Nakai S, Imanishi J, Yoshimoto T, Hisa Y, Mazda O.
    • Journal Title

      Cancer Res 69

      Pages: 2523-2530

    • Peer Reviewed
  • [Journal Article] Intracellular enzyme-responsive fragmentation of nonviral gene carriers leads to polyplex destabilization and enhanced transgene expression.2009

    • Author(s)
      Hashimoto T, Tachibana Y, Nozaki H, Mazda O, Niidome T, Murakami A, Yamaoka T.
    • Journal Title

      Chem Lett 38

      Pages: 718-719

    • Peer Reviewed
  • [Journal Article] Raspberry-like assembly of cross-linked nanogels for protein delivery.2009

    • Author(s)
      Hasegawa U, Sawada SI, Shimizu T, Kishida T, Otsuji E, Mazda O, Akiyoshi K.
    • Journal Title

      J Control Release 140

      Pages: 312-317

    • Peer Reviewed
  • [Journal Article] Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint.2008

    • Author(s)
      Tonomura H, Takahashi KA, Mazda O, Arai Y, Shin-Ya M, Inoue A, Honjo K, Hojo T, Imanishi J, Kubo T.
    • Journal Title

      J Orthop Res 26

      Pages: 34-41

    • Peer Reviewed
  • [Journal Article] Pleiotrophic functions of Epstein-Barr virus nuclear antigen-1 (EBNA-1) and oriP differentially contribute to the efficacy of transfection/expression of exogenous gene in mammalian cells.2008

    • Author(s)
      Kishida T, Asada H, Kubo K, Sato Y, Shin-Ya M, Imanishi J, Yoshikawa K, Mazda O.
    • Journal Title

      J Biotechnol 133

      Pages: 201-207

    • Peer Reviewed
  • [Journal Article] Nanogel DDS enables sustained release of IL-12 for tumor immunotherapy.2008

    • Author(s)
      Shimizu T, Kishida T, Hasegawa U, Ueda Y, Imanishi J, Yamagishi H, Akiyoshi K, Otsuji E, Mazda O.
    • Journal Title

      Biochem Biophys Res Commun 367

      Pages: 330-335

    • Peer Reviewed
  • [Journal Article] Therapeutic RNA interference of malignant melanoma by electrotransfer of small interfering RNA targeting Mitf.2007

    • Author(s)
      Nakai N, Kishida T, Shin-Ya M, Imanishi J, Ueda Y, Kishimoto S, Mazda O.
    • Journal Title

      Gene Ther 14

      Pages: 357-365

    • Peer Reviewed
  • [Journal Article] Sono- poration-mediated transduction of pDNA/siRNA into joint synovium in vivo.2007

    • Author(s)
      Saito M, Mazda O, Takahashi KA, Arai Y, Kishida T, Shin-Ya M, Inoue A, Tonomura H, Sakao K, Morihara T, Imanishi J, Kawata M, Kubo T.
    • Journal Title

      J Orthop Res 25

      Pages: 1308-1316

    • Peer Reviewed
  • [Journal Article] IL-21 administration into the nostril alleviates murine allergic rhinitis.2007

    • Author(s)
      Hiromura Y, Kishida T, Nakano H, Hama T, Imanishi J, Hisa Y, Mazda O.
    • Journal Title

      J Immunol 179

      Pages: 7157-7165

    • Peer Reviewed
  • [Journal Article] IL-21 induces inhibitor of differentiation 2 (Id2) and leads to complete abrogation of anaphylaxis in mice.2007

    • Author(s)
      Kishida T, Hiromura Y, Shin-Ya M, Asada H, Kuriyama H, Sugai M, Shimizu A, Yokota Y, Hama T, Imanishi J, Hisa Y, Mazda O.
    • Journal Title

      J Immunol 179

      Pages: 8554-8561

    • Peer Reviewed
  • [Journal Article] Interleukin-21 triggers both cellular and humoral immune responses leading to therapeutic antitumor effects against head and neck squamous cell carcinoma.2006

    • Author(s)
      Nakano H, Kishida T, Asada H, Shin-Ya M, Shinomiya T, Imanishi J, Shimada T, Nakai S, Takeuchi M, Hisa Y, Mazda O.
    • Journal Title

      J Gene Med 8

      Pages: 90-99

    • Peer Reviewed
  • [Journal Article] Transthoracic direct current (DC) shock facilitates intramyocardial transfection of naked plasmid DNA infused via coronary vessels in canines.2006

    • Author(s)
      Iida Y, Oda Y, Nakamori S, Tsunoda S, Kishida T, Gojo S, Shin-Ya M, Asada H, Imanishi J, Yoshikawa T, Matsubara H, Mazda O.
    • Journal Title

      Gene Ther 13

      Pages: 906-916

    • Peer Reviewed
  • [Journal Article] In vivo gene therapy of type I diabetic mellitus using a cationic emulsion containing an Epstein Barr virus (EBV) based plasmid vector.2006

    • Author(s)
      Yoo HS, Mazda O, Lee HY, Kim JC, Kwon SM, Lee JE, Kwon IC, Jeong H, Jeong YS, Jeong SY.
    • Journal Title

      J Control Release 112

      Pages: 139-144

    • Peer Reviewed
  • [Journal Article] Intracellular interferon triggers Jak/Stat signaling cascade and induces p53-dependent antiviral protection.2005

    • Author(s)
      Shin-Ya M, Hirai H, Satoh E, Kishida T, Asada H, Aoki F, Tsukamoto M, Imanishi J, Mazda O.
    • Journal Title

      Biochem Biophys Res Commun 329

      Pages: 1139-1146

    • Peer Reviewed
  • [Journal Article] Yoshikawa K. Folding transition into a loosely collapsed state in plasmid DNA as revealed by single-molecule observation.2005

    • Author(s)
      Sato YT, Hamada T, Kubo K, Yamada A, Kishida T, Mazda O
    • Journal Title

      FEBS Lett 579

      Pages: 3095-3099

    • Peer Reviewed
  • [Journal Article] Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart.2005

    • Author(s)
      Tsunoda S, Mazda O, Oda Y, Iida Y, Akabame S, Kishida T, Shin-Ya M, Asada H, Gojo S, Imanishi J, Matsubara H, Yoshikawa T.
    • Journal Title

      Biochem Biophys Res Commun 336

      Pages: 118-127

    • Peer Reviewed
  • [Journal Article] Electrotransfer of small interfering RNA ameliorated arthritis in rats.2005

    • Author(s)
      Inoue A, Takahashi KA, Mazda O, Terauchi R, Arai Y, Kishida T, Shin-Ya M, Asada H, Morihara T, Tonomura H, Ohashi S, Kajikawa Y, Kawahito Y, Imanishi J, Kawata M, Kubo T.
    • Journal Title

      Biochem Biophys Res Commun 336

      Pages: 903-908

    • Peer Reviewed
  • [Journal Article] Mitf silencing cooperates with IL-12 gene transfer to inhibit melanoma in mice.

    • Author(s)
      Nakai N, Kishida T, Hartmann G, Katoh N, Imanishi J, Kishimoto S, Mazda O.
    • Journal Title

      Int Immunopharmacol (in press)

    • Peer Reviewed
  • [Presentation] The Epstein-Barr virus-based episomal vectors may provide efficient means of genetic transduction in vitro and in vivo.2009

    • Author(s)
      Mazda O, Kishida T, Imanishi J.
    • Organizer
      2nd World Congress of Regenerative Medicine & Stem Cell-2009 (RMSC-2009)/4th China Medical Biotech Forum (CMBF)
    • Place of Presentation
      Dalian, China
    • Year and Date
      20090808-20090810
  • [Presentation] A strain of unicellular alga that may provide a novel functional food to decelerate senescence.2009

    • Author(s)
      Kishida T, Imanishi J, Mazda O.
    • Organizer
      3rd FEMS Congress of European Microbiologists.
    • Place of Presentation
      Gothenberg, Sweden
    • Year and Date
      20090628-20090702
  • [Presentation] Infectious capability of HSV-1 is drastically reduced by high pressure-based procedures to attenuate virus.2009

    • Author(s)
      Cui FD, Kishida T, Imanishi J, Hayakawa K, Mazda O.
    • Organizer
      3rd FEMS Congress of European Microbiologists.
    • Place of Presentation
      Gothenberg, Sweden
    • Year and Date
      20090628-20090702
  • [Presentation] Multiple functions and subcellular localization of EBV nuclear antigen-1 (EBNA-1) contributes to transfection/ 6 expression efficiencies of EBV episomal vectors in mammalian cells.2009

    • Author(s)
      Mazda O, Kishida T, Asada H, Yoshimura SH, Imanishi J.
    • Organizer
      3rd FEMS Congress of European Microbiologists.
    • Place of Presentation
      Gothenberg, Sweden
    • Year and Date
      20090628-20090702
  • [Presentation] Local administration of IL-21 suppresses nasal allergic reaction in a murine model.2009

    • Author(s)
      Hiromura Y, Mazda O, Kishida T, Hama T, Hisa Y.
    • Organizer
      The 29th Annual Midwinter Research Meeting of the Association for Research in Otolaryngology (ARO)
    • Place of Presentation
      Baltimore, MD, USA
    • Year and Date
      2009-02-05
  • [Presentation] IL-21 administration into the nostril alleviates murine allergic rhinitis.2008

    • Author(s)
      Hiromura Y, Kishida T, Nakano H, Hama T, Mazda O, Hisa Y.
    • Organizer
      The 12th Japan-Korean joint meeting of Otorhinolaryngology-Head and Neck Surgery.
    • Place of Presentation
      Nara
    • Year and Date
      2008-04-05
  • [Presentation] Cationic silica nanoparticles efficiently transfer into mammalian cells.2007

    • Author(s)
      Li L, Takenaka T, Zinchenko AA, Chen N, Inagaki S, Asada H, Kishida T, Mazda O, Murata S, Yoshikawa K.
    • Organizer
      2007 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya
    • Year and Date
      20071112-20071113
  • [Presentation] IL-27 induced antitumor effects against HNSCC in mice.2007

    • Author(s)
      Matsui M, Kishida T, Asada H, Nakano H, Imanishi J, Mazda O, Hisa Y.
    • Organizer
      AAO-HNSF 2007 Annual Meeting & OTO EXPO.
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      20070916-20070919
  • [Presentation] The therapeutic effects of inflammatory cytokine-specific small interfering RNA transduction into joints on rat arthritis.2007

    • Author(s)
      Inoue A, Takahashi KA, Arai Y, Saito M, Tonomura H, Sakao K, Mazda O, Imanishi J, Kubo T.
    • Organizer
      53rd Annual Meeting of the Orthopaedic Research Society.
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      20070211-20070214
  • [Presentation] Synergistic effect of both TRAIL gene and a low dose of anti-cancer drugs on liver metastasis.2007

    • Author(s)
      Iwai M, Ishii M, Harada Y, Mori T, Itoh Y, Okanoue T, Kishida T, Mazda O, Imanishi J.
    • Organizer
      17th Asian Pacific Association for the Study of the Liver (APASL)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-03-29
  • [Presentation] Local administration of IL-21 suppresses nasal allergic reaction in a murine model.2006

    • Author(s)
      Hiromura Y, Mazda O, Kishida T, Hama T, Hisa Y.
    • Organizer
      1st Joint Meeting of European National Societies of Immunology and 16th European Congress of Immunology.
    • Place of Presentation
      Paris, France
    • Year and Date
      2006-02-07
  • [Presentation] EBV-based artificial chromosome provides an efficient tool for gene delivery into mammalian cells.2005

    • Author(s)
      Kishida T, Imanishi J, Mazda O.
    • Organizer
      NanoBio Europe 2005
    • Place of Presentation
      Muenster, Germany.
    • Year and Date
      20050922-20050924
  • [Book] Front Biosci(Molecular therapeutics of cancer by means of electroporation-based transfer of siRNAs and EBV-based expression vectors.)2009

    • Author(s)
      Mazda O, Kishida T.
    • Total Pages
      316-331
  • [Book] Nonviral gene administration by means of the EBV-based episomal vectors and it application to gene therapy and regenerative medicine.(Summarized Report of Symposium on"System Cell Engineering by Multi-scale manipulation" at 20th Anniversary MHS2009 & Micro-Nano Global)2009

    • Author(s)
      Mazda O, Kishida T, Matsui M, Nakano H, Yoshimoto K, Shimada T, Nakai S, Imanishi J, Hisa Y.
    • Total Pages
      21-26
    • Publisher
      COE
  • [Book] Subcellular localization of EBNA1 and oriP that may support the high rate of exogenous gene transfer achieved using the EBV genetic elements.(Proceedings of the 2008 International Symposium on Micro-Nano Mechatronics and Human Science,)2008

    • Author(s)
      Asada H, Yoshimura SH, Kishida T, Imanishi J, Mazda O.
    • Total Pages
      54-59
    • Publisher
      MHS
  • [Book] Cationic silica nanoparticles are efficiently transferred into mammalian cells.(2007 International Symposium on Micro-Nano Mechatronics and Human Science,)2007

    • Author(s)
      Liu L, Takenaka T, Zinchenko AA, Chen N, Inagaki S, Asada H, Kishida T, Mazda O, Murata S, Yoshikawa, K.
    • Total Pages
      281-285
    • Publisher
      MHS
  • [Book] Interleukin-21 as an effective suppressant for IgE-mediated allergic hypersensitivity reactions.(2007 International Symposium on Micro-Nano Mechatronics and Human Science,)2007

    • Author(s)
      Kishida T, Hiromura Y, Hama T, Imanishi J, Hisa Y, Mazda O.
    • Total Pages
      304-309
    • Publisher
      MHS
  • [Book] Gene Therapy 2007.(Ochiai T, Shimada H, Tagawa M, editors., Electroporation-mediated molecular therapeutics of cancer using short interfering RNA and EBV-based DNA construct with properties of an artificial chromosome.)2007

    • Author(s)
      Mazda O, Kishida T.
    • Total Pages
      34-55
    • Publisher
      Medical View
  • [Book] Novel gene transduction procedures in vivo using the Epstein-Barr virus-based artificial chromosomes.(2006 IEEE International Symposium on Micro-Nano Mechatronics and Human Science,)2006

    • Author(s)
      Mazda O, Kishida T, Asada H, Iida Y, Tsunoda S, Oda Y, Shin-Ya M, Yoshikawa T, Matsubara H, Imanishi J.
    • Total Pages
      22-27
    • Publisher
      MHS
  • [Book] Non-viral gene therapy: gene design and delivery.(Taira K, Kataoka K, Niidome T, editors., Functional Nucleotide sequences capable of promoting nonviral genetic transfer.)2005

    • Author(s)
      Mazda O, Kishida T.
    • Total Pages
      198-210
    • Publisher
      Springer-Verlag
  • [Book] Application of EBV-based artificial chromosome to genetic engineering of mammalian cells and tissues.(Proceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century)2005

    • Author(s)
      Kishida T, Shin-Ya M, Imanishi J, Mazda O.
    • Total Pages
      124-129
    • Publisher
      COE Progr

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Published: 2011-06-18   Modified: 2016-04-21  

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