Plant micro nutrient transporters and their regulation

2009 Fiscal Year Final Research Report

• Project/Area Number: 17078004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: FUJIWARA Toru The University of Tokyo, 大学院・農学生命科学研究科, 教授 (80242163)
• Project Period (FY): 2005 – 2009
• Keywords: ホウ素 / モリブデン / 植物 / トランスポーター / 必須元素

Research Abstract

Plants grow on soil by taking essential mineral nutrients from soil and human life depends on this ability of plants. In this project, we focused on essential minor mineral nutrients. Novel transporters of boron with distinct physiological function and activities are identified and characterized. Some mechanisms of regulation of transporter expression have been revealed. Nutrient stress tolerant plants were generated with the use of mineral nutrient transporters.

Research Products

• [Journal Article] Significant contribution of boron stored in seeds to initial growth of rice seedlings. (2011)
  • Author(s): Uraguchi, S., Fujiwara, T.
  • Journal Title: Plant Soil 340 Pages: 435-442
  • Peer Reviewed
• [Journal Article] Arabidopsis thaliana 26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance (2011)
  • Author(s): Sakamoto, T., Kamiya, T., Sako, K., Yamaguchi, J., Yamagami, M., Fujiwara, T.
  • Journal Title: Bioscience Biotechnology and Biochemistry 75 Pages: 561-567
  • Peer Reviewed
• [Journal Article] Effects of molybdenum deficiency and defects in molybdate transporter MOT1 on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis thaliana. (2011)
  • Author(s): Ide Y, Kusano M, Oikawa A, Fukushima A, Tomatsu H, Saito K, Hirai MY, Fujiwara T.
  • Journal Title: J Exp Bot. 62 Pages: 1483-1497
  • Peer Reviewed
• [Journal Article] High boron-induced ubiquitination regulates vacuolar sorting of the BOR1 borate transporter in Arabidopsis thaliana. (2011)
  • Author(s): Kasai K, Takano J, Miwa K, Toyoda A, Fujiwara T.
  • Journal Title: J Biol Chem. 286 Pages: 6175-6183
  • Peer Reviewed
• [Journal Article] Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways. (2010)
  • Author(s): Takano, J., Tanaka, M., Toyoda, A., Miwa, K., Kasai, K., Fuji, K., Onouchi, H., Naito, S., Fujiwara, T.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 17 Pages: 5220-5225
  • Peer Reviewed
• [Journal Article] Mechanism of boron tolerance in soil bacteria. (2010)
  • Author(s): Ahmed, I., Fujiwara, T.
  • Journal Title: Canad.J.Microbiol. 56 Pages: 22-26
  • Peer Reviewed
• [Journal Article] Possible involvement of ploidy in tolerance to boron deficiency in Arabidopsis thaliana. (2010)
  • Author(s): Kasajima, I., Yoshizumi, T., Ichikawa, T., Matsui, M., Fujiwara, T.
  • Journal Title: Plant Biotechnology 27 Pages: 435-445
  • Peer Reviewed
• [Journal Article] Boron transport in plants : co-ordinated regulation of transporters. (2010)
  • Author(s): Miwa, K., Fujiwara, T.
  • Journal Title: Ann.Bot. 105 Pages: 1103-1108
  • Peer Reviewed
• [Journal Article] Rhodococcus baikonurensis BTM4c, a Boron-Tolerant Actinobacterial Strain Isolated from Soil. (2010)
  • Author(s): Yoon, J., Miwa, H., Ahmed, I., Yokota, A., Fujiwara, T.
  • Journal Title: Biosci.Biotechnol.Biochemi. 74 Pages: 178-181
  • Peer Reviewed
• [Journal Article] WRKY6 is involved in the response to low boron conditions in Arabidopsis thaliana. (2010)
  • Author(s): Kasajima, I., Ide, Y., Hirai-Yokota, M., Fujiwara, T.
  • Journal Title: Physiologia Plantarum 139 Pages: 80-92
  • Peer Reviewed
• [Journal Article] The intracellular transport of transporters : membrane trafficking of mineral transporters. (2009)
  • Author(s): Fuji, K., Miwa, K., Fujiwara, T.
  • Journal Title: Current Opinion in Plant Biology 12 Pages: 699-704
  • Peer Reviewed
• [Journal Article] Arabidopsis NIP1 ; 1 Transports Antimonite and Determines Antimonite Sensitivity. (2009)
  • Author(s): Kamiya, T., Fujiwara, T.
  • Journal Title: Plant and Cell Physiology 50 Pages: 1977-1981
  • Peer Reviewed
• [Journal Article] Highly boron deficiency tolerant plants generated by enhanced expression of NIP5 ; 1, a boric acid channel. (2009)
  • Author(s): Kato Y, Miwa K, Takano J, Wada M, Fujiwara T.
  • Journal Title: Plant Cell Physiol. 50 Pages: 58-66
  • Peer Reviewed
• [Journal Article] NIP1 ; 1, an Aquaporin Homolog, Determines the Arsenite Sensitivity of Arabidopsis thaliana. (2009)
  • Author(s): Kamiya, T., Fujiwara, T.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 2114-2120
  • Peer Reviewed
• [Journal Article] NIP6 ; 1 is a Boric Acid Channel for Preferential Transport of Boron to Growing Shoot Tissues in Arabidopsis thaliana. (2008)
  • Author(s): Tanaka M, Wallace I, Takano J, Roberts RM, Fujiwara T.
  • Journal Title: Plant Cell 20 Pages: 2860-2875
  • Peer Reviewed
• [Journal Article] Plants tolerant of high boron levels. (2007)
  • Author(s): Miwa, K., Takano, J., Omori, H. Seki, M., Shonozaki, K., Fujiwara, T.
  • Journal Title: Science 318 Pages: 1417
  • Peer Reviewed
• [Journal Article] Physiological roles and transport mechanisms of boron : perspectives from plants. (2007)
  • Author(s): Tanaka, M., Fujiwara, T.
  • Journal Title: Pflug.Arch. 456 Pages: 671-677
  • Peer Reviewed
• [Journal Article] An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil. (2007)
  • Author(s): Tomatsu, H., Takano, J., Takahashi, H., Watanabe-Takahashi A., Shibagaki, N., Fujiwara, T.
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 18807-18812
  • Peer Reviewed
• [Journal Article] Cell-type specificity of the expression of Os BOR1, a rice efflux boron transporter gene, is regulated in response to boron availability for efficient boron uptake and xylem loading. (2007)
  • Author(s): Nakagawa, Y., Hanaoka, H., Kobayashi, M., Miyoshi, K., Miwa, K., Fujiwara, T.
  • Journal Title: Plant Cell 19 Pages: 2624-2635
  • Peer Reviewed
• [Journal Article] An efflux transporter of silicon in rice. (2007)
  • Author(s): Ma, J.F., Yamaji, N., Mitani, N., Tamai, K., Konishi, S., Fujiwara, T.,Katsuhara, M., Yano, M.
  • Journal Title: Nature 448 Pages: 209-212
  • Peer Reviewed
• [Journal Article] Identification of novel Arabidopsis thaliana genes which are induced by high levels of boron. (2007)
  • Author(s): Kasajima, I., Fujiwara, T.
  • Journal Title: Plant Biotechnology 24 Pages: 355-360
  • Peer Reviewed
• [Journal Article] Saccharomyces cerevisiae BOR1p is a boron exporter and a key determinant of boron torelance. (2007)
  • Author(s): Takano, J., Kobayashi, M., Noda, Y., Fujiwara, T.
  • Journal Title: FEMS Microbiology Letters 267 Pages: 230-235
  • Peer Reviewed
• [Journal Article] Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae (2007)
  • Author(s): Akira Nozawa, Junpei Takano, Masaharu Kobayashi, Nicolaus von Wiren, Toru Fujiwara
  • Journal Title: FEMS Microbiology Letters 262 Pages: 216-222
  • Peer Reviewed
• [Journal Article] Improvement of seed yields under boron-limiting conditions through overexpression of BOR1, a boron transporter for xylem loading, in Arabidopsis thaliana. (2006)
  • Author(s): Miwa, K., Takano, J., Fujiwara, T.
  • Journal Title: Plant J. 46 Pages: 1084-1091
  • Peer Reviewed
• [Journal Article] The Arabidopsis major intrinsic protein NIP5 ; 1 is essential for efficient boron uptake and plant development under boron limitation. (2006)
  • Author(s): Takano, J., Wada, M., Ludewig, U., Schaaf, G., von Wiren, N., Fujiwara, T.
  • Journal Title: Plant Cell 18 Pages: 1498-1509
  • Peer Reviewed
• [Presentation] Regulation of mRNA accumulation of NIP5 ; 1 in response to boron. (2011)
  • Author(s): 藤原徹
  • Organizer: 日中植物栄養ワークショップ
  • Place of Presentation: 倉敷
  • Year and Date: 2011-03-30
• [Presentation] Roles of Sultr5 ; 1(MOT2) and Sultr1 ; 2 in Mo transport and distribution in Arabidopsis thaliana. (2010)
  • Author(s): 藤田春佳
  • Organizer: 8th Int'l Workshop "Sulfur Metabolism in Higher Plants"
  • Place of Presentation: メルボルン,オーストラリア
  • Year and Date: 2010-11-22
• [Presentation] Mechamisms of boron-regulated expression of NIP5 ; 1, a boric acid channel, in Arabidopsis thaliana. (2010)
  • Author(s): Mayuki Tanaka, Toru Fujiwara.
  • Organizer: International Workshop on Plant Membrane Biology XV
  • Place of Presentation: アデレード,オーストラリア
  • Year and Date: 2010-09-21
• [Presentation] Boron-induced degradation of the borate transporter BOR1 is regulated by ubiquitination in Arabidopsis. (2010)
  • Author(s): Koji Kasai, Junpei Takano, Atsushi Toyoda, Kyoko Miwa, Toru Fujiwara.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-06
• [Presentation] ホウ素過剰感受性を示すシロイヌナズナのコンデンシンII変異体の解析-DNA損傷の可能性 (2010)
  • Author(s): 坂本卓也、乾(辻本)弥生、藤原徹
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-18
• [Presentation] シロイヌナズナホウ酸トランスポーターBOR4のホウ酸条件による発現応答 (2009)
  • Author(s): 三輪京子、藤原徹
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-09-15
• [Presentation] Identification and characterization of Arabidopsis molybdate transporters. (2008)
  • Author(s): 藤原徹
  • Organizer: 日中植物栄養ワークショップ
  • Place of Presentation: 北京
  • Year and Date: 2008-10-10
• [Presentation] ホウ素トランスポーターの発現によるトマトのホウ素欠乏耐性付与 (2008)
  • Author(s): 藤原徹
  • Organizer: 日本土壌肥料学会2008年大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-10
• [Presentation] Toward comprehensive understanding of regulatory network of sulfur metabolism. (2008)
  • Author(s): 藤原徹
  • Organizer: 7th Workshop on Sulfur Metabolism in Plants.
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2008-05-15
• [Presentation] Roles of Arabidopsis thaliana NIP6 ; 1 for preferential transporter of boron to young leaves. (2007)
  • Author(s): Mayuki Tanaka, Junpei Takano, Ian S.Wallace, Daniel M.Roberts, Toru Fujiwara.
  • Organizer: XIV International Workshop on Plant Membrane Biology
  • Place of Presentation: Valencia
  • Year and Date: 2007-06-25
• [Presentation] Roles of channels and transporters in regulation of boron transport. (2007)
  • Author(s): 藤原徹, Junpei Takano, Mayuki Tanaka, Kyoko Miwa, Hideki Hanaoka.
  • Organizer: XIV International Workshop on Plant Membrane Biology
  • Place of Presentation: Valencia, Spain
  • Year and Date: 2007-06-25
• [Remarks] ホームページ等