Channel micro-assembly in junctional membrane complexes

2009 Fiscal Year Final Research Report

• Project/Area Number: 17081001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University (2006-2009); Tohoku University (2005)
• Principal Investigator: TAKESHIMA Hiroshi Kyoto University, 薬学研究科, 教授 (70212024)
• Project Period (FY): 2005 – 2009
• Keywords: ジャンクトフィリン / 結合膜構造 / 小胞体Ca^<2+>放出

Research Abstract

The aim of this project is to understand the molecular basis of channel crosstalk in junctional membrane complexes between the plasma membrane and the endo-/sarcoplasmic reticulum. In particular, this project focused on i) biological roles of junctophilin subtypess as proteins producing junctional membrane complexes, ii) physiological abnormalities observed in knockout mice lacking junctophilins, iii) human diseases caused by mutated junctophilins, and iv) the new mechanisms supporting the junctophilin-mediated channel crosstalk.

Research Products

• [Journal Article] Essential role of TRIC-B channel in Ca^<2+>-handling of alveolar epithelium and perinatal lung maturation. (2009)
  • Author(s): Yamazaki, D., Komazaki, S., Nakanishi, H., Mishima, A., Nishi, M., Yazawa, M., Yamazaki, T., Taguchi, R., Takeshima, H.
  • Journal Title: Development 136 Pages: 2355-2361
  • Peer Reviewed
• [Journal Article] (*co-corresponding authors) MG53 nucleates assembly of cell membrane repair machinery. (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, J-K., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H.*, Ma, J.*
  • Journal Title: Nature Cell Biol. 11 Pages: 56-64
  • Peer Reviewed
• [Journal Article] Single-channel characterization of the rabbit recombinant RyR2 reveals a novel inactivation property of physiological concentrations of ATP. (2008)
  • Author(s): Stewart, R., Song, L., Carter, S.M., Sigalas, C., Zaccai, N.R., Kanamarlapudi, V., Bhat, M.B., Takeshima, H., Sitsapesan, R.
  • Journal Title: J. Membr. Biol. 222 Pages: 65-77
  • Peer Reviewed
• [Journal Article] Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins. (2007)
  • Author(s): Ikeda, A., Miyazaki, T., Kakizawac, S., Okuno, Y., Tsuchiya, S., Myomoto, A., Saito, S., Yamamoto, T., Yamazaki, T., Iino, M., Tsujimoto, G., Watanabe, M., Takeshima, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 363 Pages: 835-839
  • Peer Reviewed
• [Journal Article] TRIC channels are essential for Ca^<2+> handling in intracellular stores. (2007)
  • Author(s): Yazawa, M., Ferrante, C., Feng, J., Mio, K., Ogura, T., Zhang, M., Lin, P-H., Pan, Z., Komazaki, S., Kato, K., Nishi, M., Zhao, X., Weisleder, N., Sato, C., Ma, J., Takeshima, H.
  • Journal Title: Nature 448 Pages: 78-82
  • Peer Reviewed
• [Journal Article] Mutations of junctophilin type 2 associated with hypertrophic cardiomyopathy. (2007)
  • Author(s): Matsushita, Y., Furukawa, T., Kasanuki, H., Nishibatake, M., Kurihara, Y., Ikeda, A., Kamatani, N., Takeshima, H., Matsuoka, R.
  • Journal Title: J. Hum. Genet. 52 Pages: 543-548
  • Peer Reviewed
• [Journal Article] Calumin, a novel Ca^<2+>-binding transmembrane protein on the endoplasmic reticulum. (2007)
  • Author(s): Zhang, M., Yamazaki, T., Yazawa, M., Treves, S., Nishi, M., Murai, M., Shibata, E., Zorzato, F., Takeshima, H.
  • Journal Title: Cell Calcium 42 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. (2007)
  • Author(s): Kakizawa, S., Kishimoto, Y., Hashimoto, K., Miyazaki, T., Furutani, K., Shimizu, H., Fukaya, M., Nishi, M., Sakagami, H., Ikeda, A., Kondo, H., Kano, M., Watanabe, M., Iino, M., Takeshima, H.
  • Journal Title: EMBO J. 26 Pages: 1924-1933
  • Peer Reviewed
• [Journal Article] Uncoupling of store-operated Ca^<2+> entry and altered Ca^<2+> release from sarcoplasmic reticulum through silencing of junctophilins. (2006)
  • Author(s): Hirata, Y., Brotto, M., Weisleder, N., Chu, Y., Lin, P., Zhao, X., Thornton, A., Komazaki, S., Takeshima, H., Ma, J., Pan, Z.
  • Journal Title: Biophys. J. 90 Pages: 4418-4427
  • Peer Reviewed
• [Journal Article] Functional uncoupling between Ca^<2+> release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins. (2006)
  • Author(s): Moriguchi, S., Nishi, M., Komazaki, S., Sakagami, H., Miyazaki, T., Masumiya, H., Saito, S., Watanabe, M., Kondo, H., Yawo, H., Fukunaga, K., Takeshima, H.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 10811-10816
  • Peer Reviewed
• [Presentation] Immuno-proteomic approach to E-C coupling: junctophilin and TRIC channel in cardiac Ca^<2+> release (2009)
  • Author(s): Takeshima, H.
  • Organizer: IUPS Whole-day Symposium
  • Place of Presentation: in Kyoto
  • Year and Date: 2009-07-28
• [Presentation] TRIC channel subtypes and SR/ER Ca^<2+> release. (2009)
  • Author(s): Takeshima, H.
  • Organizer: Gordon Research Conference; Muscle Excitation-Contraction Coupling
  • Place of Presentation: in Waterville Valley, USA
  • Year and Date: 2009-06-18
• [Presentation] Ryanodine receptor-mediated Ca^<2+> release from sarcoplasmic reticulum (2006)
  • Author(s): Takeshima, H.
  • Organizer: Gordon Research Conference; Muscle Excitation-Contraction Coupling
  • Place of Presentation: in New London, USA
  • Year and Date: 2006-06-09
• [Presentation] Mitsugumins, a series of novel membrane proteins in skeletal muscle (2006)
  • Author(s): Takeshima, H.
  • Organizer: Muscle Symposium in Basel
  • Place of Presentation: in Basel, Switzerland
  • Year and Date: 2006-05-05
• [Presentation] Junctophilin-mediated channel coupling during muscle E-C coupling (2005)
  • Author(s): Takeshima, H.
  • Organizer: European Muscle Conference
  • Place of Presentation: in Debrecen, Hungary
  • Year and Date: 2005-09-17
• [Book] Junctophilin. Encyclopedia of movement disorders. (2009)
  • Author(s): Takeshima, H.
  • Total Pages: 92-95
  • Publisher: Elsevier Limitted
• [Book] 医歯薬系学生のための基礎生命科学 (2008)
  • Author(s): 竹島浩、山崎哲男、池田篤史、輿水崇鏡
  • Total Pages: 214
  • Publisher: 京都廣川書店
• [Book] イオンチャネル最前線(リアノジン受容体, 医学のあゆみ別冊) (2005)
  • Author(s): 竹島浩
  • Total Pages: 171-175
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/