Analytical studies on signal transduction pathways of phot2 in chloroplast photorelocation movement

2009 Fiscal Year Final Research Report

• Project/Area Number: 17084006
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University (2008-2009); National Institute for Basic Biology (2005-2007)
• Principal Investigator: WADA Masamitsu Kyushu University, 大学院・理学研究院, 特任教授 (60011681)
• Co-Investigator(Kenkyū-buntansha): KANEGAE Takeshi 首都大学東京, 理工学研究科, 助教 (70264588)
• Project Period (FY): 2005 – 2009
• Keywords: 環境応答 / 光 / 青色光 / 光受容体 / 葉緑体 / 運動 / フォトトロピン

Research Abstract

Phototropin2 is a blue light receptor mediating chloroplast avoidance response but at the same time it mediates chloroplast accumulation response as phot1 does. The purpose of this work is to clarify how phot2 can mediate the both contradictory phenomena. Our results suggest that phot2 locates both on the plasma membrane and on chloroplast envelope but the only latter one can mediate chloroplast avoidance response.

Research Products

• [Journal Article] The speed of intracellular signal transfer for chloroplast movement (2010)
  • Author(s): Tsuboi, H., M. Wada.
  • Journal Title: Plant Signal & Behavior (in press)
• [Journal Article] Chloroplasts do not have a polarity for light-induced accumulation movement (2009)
  • Author(s): Tsuboi, H., H. Yamashita, M. Wada.
  • Journal Title: J. Plant Research 122 Pages: 131-140
  • Peer Reviewed
• [Journal Article] Simultaneous visualization of two protein complexes in a single plant cell using multicolor fluorescence complementation analysis (2009)
  • Author(s): Kodama, Y., M. Wada.
  • Journal Title: Plant Mol. Biol. 70 Pages: 211-217
  • Peer Reviewed
• [Journal Article] Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis (2009)
  • Author(s): Kadota, A., N. Yamada, N. Suetsugu, M. Hirose, C. Saito, K. Shoji, S. Ichikawa, T. Kagawa, A. Nakano, M. Wada.
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 13106-13111
  • Peer Reviewed
• [Journal Article] Blue light-induced phototropism of inflorescence stems and petioles is mediated by phototropin family members phot1 and phot2 (2009)
  • Author(s): Kagawa, T., M. Kimura, M. Wada.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1774-1785
  • Peer Reviewed
• [Journal Article] Speed of signal transfer in the chloroplast accumulation response. (2009)
  • Author(s): Tsuboi, H., M. Wada.
  • Journal Title: J. Plant Research 123(in press)
  • Peer Reviewed
• [Journal Article] Blue light diminishes interaction of PAS/LOV proteins, putative blue light receptors in Arabidopsis thaliana, with their interacting partners (2008)
  • Author(s): Ogura, Y., A. Komatsu, K. Zikihara, T. Nanjo, S. Tokutomi, M. Wada, T. Kiyosue.
  • Journal Title: J. Plant Research 121 Pages: 97-105
  • Peer Reviewed
• [Journal Article] Low temperature-induced chloroplast relocation mediated by a blue light receptor, phototropin 2, in fern gametophytes (2008)
  • Author(s): Kodama, Y., H. Tsuboi, T. Kagawa, M. Wada.
  • Journal Title: J. Plant Research 121 Pages: 441-448
  • Peer Reviewed
• [Journal Article] Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement (2008)
  • Author(s): Oikawa, K., A. Yamasato, S. -G. Kong, M. Kasahara, M. Nakai, F. Takahashi, Y. Ogura, T. Kagawa, M. Wada
  • Journal Title: Plant Physiol. 148 Pages: 829-842
  • Peer Reviewed
• [Journal Article] PAS/LOV proteins: A proposed new class of plant blue light receptor (2008)
  • Author(s): Ogura, Y., S. Tokutomi, M. Wada, T. Kiyosue.
  • Journal Title: Plant Signaling Behavior 3 Pages: 966-968
• [Journal Article] Fern as a model system to study photomorphogenesis. (2007)
  • Author(s): Wada, M.
  • Journal Title: J. Plant Research 120 Pages: 3-16
  • Peer Reviewed
• [Journal Article] Phytochromedependent photomovement responses mediated by phototropin family proteins in Cryptogam plants (2007)
  • Author(s): Suetsugu, N., M. Wada.
  • Journal Title: Photochem. Photobiol. 83 Pages: 87-93
  • Peer Reviewed
• [Journal Article] Chloroplast photorelocation movement mediated by phototropin family proteins in green plants (2007)
  • Author(s): Suetsugu, N., M. Wada.
  • Journal Title: Biological Chemistry 388 Pages: 927-935
  • Peer Reviewed
• [Journal Article] Phototropins and neochrome1 mediate nuclear movement in the fern Adiantum capillus-veneris (2007)
  • Author(s): Tsuboi, H., N. Suetsugu, H. Kawai -Toyooka M. Wada.
  • Journal Title: Plant Cell Physiol. 48 Pages: 892-896
  • Peer Reviewed
• [Journal Article] AUREOCHROME a photoreceptor required for photomorphogenesis in stramenopiles (2007)
  • Author(s): Takahashi, F., D. Yamagata, M. Ishikawa, Y. Fukamatsu, Y. Ogura, K. Kasahara, T. Kiyosue, M. Kikuyama, M. Wada, H. Kataoka.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 19625-19630
  • Peer Reviewed
• [Journal Article] The fern Adiantum capillus-veneris lacks stomatal responses to blue light (2006)
  • Author(s): Doi, M., M. Wada, K. Shimazaki.
  • Journal Title: Plant Cell Physiol. 47 Pages: 748-755
  • Peer Reviewed
• [Journal Article] Negative phototropic response of rhizoid cells in the fern Adiantum capillus-veneris. (2006)
  • Author(s): Tsuboi, H., N. Suetsugu, M. Wada.
  • Journal Title: J. Plant Research 119 Pages: 505-512
  • Peer Reviewed
• [Journal Article] A single chromoprotein with triple chromophores acts as both a phytochrome and a phototropin (2006)
  • Author(s): Kanegae, T., E. Hayashida, C. Kuramoto, M. Wada.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 17997-18001
  • Peer Reviewed
• [Journal Article] Analysis of expressed sequence tags in prothallia of Adiantum capillus-veneris (2005)
  • Author(s): Yamauchi, D., K. Sutoh, H. Kanegae, T. Horiguchi, K. Matsuoka, H. Fukuda, M. Wada.
  • Journal Title: J. Plant Research 118 Pages: 223-227
  • Peer Reviewed
• [Journal Article] An auxilin-like J-domain protein, JAC1, regulates phototropin-mediated chloroplast movement in Arabidopsis thaliana (2005)
  • Author(s): Suetsugu, N., T. Kagawa, M. Wada.
  • Journal Title: Plant Physiology 139 Pages: 151-162
  • Peer Reviewed
• [Journal Article] A chimeric photoreceptor gene, NEOCHROME, has arisen twice during plant evolution. (2005)
  • Author(s): Suetsugu, N., F. Mittmann, G. Wagner, J. Hughes, M. Wada.
  • Journal Title: Proc. Natl. Acad. Sci. USA 102 Pages: 13705-13709
  • Peer Reviewed
• [Presentation] CHLROPLAST MOVEMENT is mediated by phototropin family proteins, but how? (2009)
  • Author(s): Wada, M.
  • Organizer: Comemorative symposium for International Prize for Biology, Kyoto
  • Place of Presentation: Japan
  • Year and Date: 20091102-20091103
• [Presentation] The mechanism of chloroplast photorelocation movement. (2009)
  • Author(s): Wada, M.
  • Organizer: 9th IPMB Congress
  • Place of Presentation: St. Louis, Missouri, USA
  • Year and Date: 20091025-20091030
• [Presentation] Precise analyses of chloroplast behavior during chloroplast photorelocation movement (2009)
  • Author(s): Wada, M.
  • Organizer: 13th Congress of European Society for Photobiology
  • Place of Presentation: Wroclaw, Poland
  • Year and Date: 20090905-20090910
• [Presentation] Chloroplast move towards or away from light, why and how? (2009)
  • Author(s): Wada, M.
  • Organizer: 15th International Congress on Photobiology, Dusseldorf
  • Place of Presentation: Germany(Presidential lecture)
  • Year and Date: 20090618-20090623
• [Presentation] Chloroplast photorelocation movement. (2009)
  • Author(s): Wada, M.
  • Organizer: Chloroplast Workshop at Goeteborg
  • Place of Presentation: Sweden
  • Year and Date: 20090608-20090913
• [Presentation] The Mechanisms of Chloroplast Photorelocation Movement, Keystone Symposium‘Plant Sensing, Response and Adaptation to the Environment' (2009)
  • Author(s): Wada, M.
  • Organizer: Big Sky
  • Place of Presentation: Montana, USA
  • Year and Date: 20090111-20090116
• [Presentation] Chloroplast can move to any direction, How? Symposium 28 Plant Cell Biology (2008)
  • Author(s): Wada, M.
  • Organizer: The 9th International Congress on Cell Biology‘Challenge of Life Sciences: Molecules and Cells'
  • Place of Presentation: COEX, Seoul, Korea
  • Year and Date: 20081007-20081010
• [Presentation] Wada Low temperature-induced chloroplast relocation is mediated by a blue light receptor, phototropin 2 (2008)
  • Author(s): Kodama, Y., H. Tsuboi, T. Kagawa, M.
  • Organizer: in fern gametophytes. Annual meeting of American Society of Plant Biologists
  • Place of Presentation: Merida, Mexico
  • Year and Date: 20080626-20080701
• [Presentation] How actin works in chloroplast photorelocation movement? (2008)
  • Author(s): Wada, M.
  • Organizer: Symposium: Photobiology in Asia (Org. P.S. Song). Annual meeting of American Society of Photobiology
  • Place of Presentation: Burlingame, CA. USA
  • Year and Date: 20080620-20080625
• [Presentation] Chloroplasts can move back and forth, why? (2008)
  • Author(s): Wada, M.
  • Organizer: 25th Annual Missouri Symposium: Plant Photobiology
  • Place of Presentation: University of Missouri
  • Year and Date: 20080528-20080531
• [Presentation] Phototropin function in chloroplast movement through chloroplast actin (2008)
  • Author(s): Wada, M.
  • Organizer: Gordon Research Conference at Ventura
  • Place of Presentation: California, USA.
  • Year and Date: 20080127-20080202
• [Presentation] The mechanism of chloroplast photo-orientation movement. (2008)
  • Author(s): Wada, M.
  • Organizer: 4th AOCP
  • Place of Presentation: Varanasi, India
  • Year and Date: 2008-11-24
• [Presentation] Photoperception and the mechanism of chloroplast photorelocation movement. (2008)
  • Author(s): Wada, M.
  • Organizer: The Ins and Outs of Chloroplasts
  • Place of Presentation: Osaka University, Osaka
  • Year and Date: 2008-10-14
• [Presentation] Photoreceptors and their downstream targets for chloroplast movement in Physcomitrella patens. (2008)
  • Author(s): Wada, M.
  • Organizer: Tampere
  • Place of Presentation: Finland
  • Year and Date: 2008-08-18
• [Presentation] The mechanism of chloroplast photorelocation movement. (2007)
  • Author(s): Wada, M.
  • Organizer: International symposium on "Light and Life"
  • Place of Presentation: Hyderabad, India
  • Year and Date: 20070829-20070831
• [Presentation] Chloroplast photorelocasiton movement: its photoperception and moving mechanisms (2007)
  • Author(s): Wada, M.
  • Organizer: International Congress on Plant Mitochondrial Biology
  • Place of Presentation: Nara, Japan
  • Year and Date: 20070625-20070629
• [Presentation] What is the mechanism of chloroplast photoorientation movement? Origin and Evolution of Mitochondria and Chloroplasts (2007)
  • Author(s): Wada, M.
  • Organizer: FEBS Advanced Lecture Course 2007, Acquafredda di Maratea
  • Place of Presentation: Italy
  • Year and Date: 20070424-20070429
• [Presentation] Molecular mechanism of chloroplast photorelocation movement (2007)
  • Author(s): Wada, M., N. Suetsugu, S. -G. Kong
  • Organizer: The 18th International Conference on Arabidopsis Research
  • Place of Presentation: Beijing, China
  • Year and Date: 2007-06-22
• [Presentation] The mechanism of chloroplast photorelocation movement (2006)
  • Author(s): Wada, M.
  • Organizer: 3rd AOCP at Beijing
  • Year and Date: 20061117-20061120
• [Presentation] How to move chloroplast using actin filaments (2006)
  • Author(s): Wada, M
  • Organizer: Gordon Research Conference, Photosensory Receptors & Signal Transduction, Il Ciocco, Barga
  • Place of Presentation: Italy
  • Year and Date: 20060430-20060505
• [Presentation] Neochrome, a chimera photoreceptor of phytochrome and phototropin, in fern and Mougeotia (2006)
  • Author(s): Wada, M.
  • Organizer: International Plant Photobiology Meeting, Carre des Sciences
  • Place of Presentation: Paris, France
  • Year and Date: 20060424-20060428
• [Presentation] The function of phototropins and their signal transduction pathways (2006)
  • Author(s): Wada, M.
  • Organizer: International Congress of Plant Molecular Biology
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2006-08-21
• [Presentation] The Importance and the Mechanisms of Chloroplast Photorelocation Movement (2006)
  • Author(s): Wada, M
  • Organizer: GRC on Mitochondria and Chloroplast at Magdalen College
  • Place of Presentation: Oxford
  • Year and Date: 2006-08-15
• [Presentation] Photoreceptors of chloroplast photo-orientation movement (2005)
  • Author(s): Wada, M.
  • Organizer: The 21st Symposium in Conjunction with Award of International Prize of Biology Awardee: Prof. Nam-Hai Chua (Rockefeller University), Noyori Conference Hall
  • Place of Presentation: Nagoya University
  • Year and Date: 20051130-20051201
• [Presentation] Diversity of photoreceptors and motility systems in chloroplast movement (2005)
  • Author(s): Wada, M.
  • Organizer: Annual meetings of American Society of Plant Biology
  • Place of Presentation: Seattle, Washington, USA.
  • Year and Date: 2005-07-20
• [Book] Chloroplast photorelocation movement(In: Plant Cell Monographs, The Chloroplast -Interaction with Environmen)(Ed. A.S. Sandelius and H. Aronsson) (2009)
  • Author(s): Suetsugu, N., M. Wada.
  • Total Pages: 235-266
  • Publisher: Sprinter
• [Book] Photoresponses in fern gametophytes(Ed. Tom A. Ranker and Christopher H. Haufler, The Biology and Evolution of Ferns and Lycophytes) (2008)
  • Author(s): Wada, M.
  • Total Pages: 3-48
  • Publisher: Cambridge Univ.Press.
• [Book] Photomorphogenesis of Ferns.(Ed.Schafer and Nagy, Photomorphogenesis in Plants 3rd Edition.) (2006)
  • Author(s): Kanegae, T., M. Wada.
  • Total Pages: 515-536
  • Publisher: Kluwer Academic Publishers
• [Book] Photoreceptor Gene Families in Lower Plants(Ed. W.R. Briggs and J.L. Spudich, Handbook of Photosensory Receptors.) (2005)
  • Author(s): Suetsugu, N., M. Wada.
  • Total Pages: 349-369
  • Publisher: Wiley-VCH Verlag
• [Book] Chloroplast movement(Ed. Wada, M., K. Shimazaki, M. Iino., Light Sensing in Plants) (2005)
  • Author(s): Wada, M.
  • Total Pages: 193-199
  • Publisher: Springer- Verlag
• [Remarks]
  • URL: http://wadalab.biology.kyushu-u.ac.jp/
• [Remarks] 報道関連情報
• [Remarks] 西日本新聞2010年1月12日子育て夢ひろば[4]学校教育・NIE「理科大好き=葉緑体」
• [Remarks] 岐阜新聞2010年1月7日夕刊サイエンス「強い光から逃げる葉緑体」
• [Remarks] 山形新聞2009年12月28日科学欄「葉緑体で字が書けた強い光に逃げる性質利用」
• [Remarks] Science 326: 1058, 2009年11月20日号NEWS FOCUS欄「Chloroplast shuffle」
• [Remarks] 朝日新聞朝刊科学欄2009年8月11日号「葉緑体に新たな運動機構」
• [Remarks] 日刊工業新聞2009年3月6日号「みどりの学術賞」和田氏/矢原氏に7) EICネット国内ニュース2009年3月5日号平成21年(第3回)「みどりの学術賞」受賞者決定