2007 Fiscal Year Final Research Report Summary
Development of new methods for risk assessment on multi-chemical exposure to human fetus
| Project/Area Number |
17201013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Chiba University |
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Principal Investigator |
MORI Chisato Chiba University, graduate school of medicine, professor (90174375)
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| Co-Investigator(Kenkyū-buntansha) |
FUKATA Hideki Chiba University, graduate school of medicine, associate professor (00359598)
SHIMOJO Naoki Chiba University, graduate school of medicine, Lecturer (40221303)
KOMIYAMA Masatoshi Chiba University, Center for Environment, Health and Field Sciences, lecturer (70175339)
MATSUNO Yoshiharu Chiba University, graduate school of medicine, assistant professor (00376378)
TODAKA Emiko Chiba University, center for environment, health and field sciences, assistant professor (30334212)
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| Project Period (FY) |
2005 – 2007
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| Keywords | human umbilical cord / environmental chemicals / PCBs / DNA microarray / twin |
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| Research Abstract |
1. Analysis of gene expression and assessment of exposure level of environmental chemicals of umbilical cord: To investigate association of gene expression and exposure level of umbilical cord, five pair s of twin were used. It is found that maternal body, one of the environmental factors, affected gene expression. When human umbilical vein smooth muscle cells (HUVSMC) were exposed to polychlorinated biphenyls (coplanar PCBs: PCB77 and PCB126, non-coplanar PCBs: PCB153) and 3-methylcholanthrene (3MC, one of the dioxin-like compound), eNOS, NPPB, and IL-6 were down regulated by DNA microarray and real-time PCR. Because these genes involved blood vessel construction, it may be affected to fetal development.
2. Because it is thought that chemicals affect to epigenetics of DNA, we investigated on Dmnts, DNA methylation enzymes by in situ hybridization and immunohistochemistry.
3. To know current situation of PCB exposure of Japanese, we analyzed blood PCB levels of 406 people including infants. PCB levels of infant with breast milk were higher than without breast milk. Adult PCB levels increased as age. PCB was indicator as chlorinated chemicals.
4. To investigate association of chemical exposure in utero and allergy, we constructed follow up system using bank system of umbilical cord.
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[Journal Article] Fetal exposure to phytoestrogens-the difference of phytoestrogen status in mother and fetus.2005
Author(s)
Todaka, E., Sakurai, K., Fukata, H., Miyagawa, H., Uzuki, M., Omori, M., Osada, H., Ikezuki, Y., Tsutsumi, O., Iguchi, T., Mori, C.
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Journal Title
Environ. Res. 99(2)
Pages: 195-203
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to diethylstilbestrol.2005
Author(s)
Yamazaki, K., Fukata, H., Adachi, T., Tainaka, H., Kohda, M., Yamazaki, M., Kojima, K., Chiba, K., Mori, C., Komiyama, M.
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Journal Title
Mol. Reprod. Dev. 72(3)
Pages: 291-298
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Fetal exposure to phytoestrogens - the difference of phytoestrogen status in mother and fetus2005
Author(s)
Todaka, E., Sakurai, K., Fukata, H., Miyagawa, H., Uzuki, M., Omori, M., Osada, H., Ikezuki, Y., Tsutsumi, O., Iguchi, T., Mori, C.
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Journal Title
Environ Res. 99
Pages: 195-203
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to diethylstilbestrol2005
Author(s)
Yamazaki, K., Fukata, H., Adachi, T., Tainaka, H., Kohda, M., Yamazaki, M., Kojima, K., Chiba, K., Mori, C., Komiyama, M.
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Journal Title
Mol. Reprod Dev. 72
Pages: 291-298
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Measurement of blood IgE in individuals exposed to formaldehyde2006
Author(s)
K. Ohmichi, M. Komiyama, Y. Matsuno, Y. Sawabe, H. Miyaso, E. Todaka, H. Fukata, M. Ohmichi, T. Kadota, F. Nomura and C. Mori
Organizer
Society of Toxicology 45th Annual Meeting and ToxExpo
Place of Presentation
California, USA
Year and Date
20060305-09
Description
「研究成果報告書概要(和文)」より
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[Presentation] Measurement of blood IgE in individuals exposed to formaldehyde2006
Author(s)
K. Ohmichi, M. Komiyama, Y. Matsuno, Y. Sawabe, H. Miyaso, E. Todaka, H. Fukata, M. Ohmichi, T. Kadota, F. Nomura and C. Mori.
Organizer
Society of Toxicology 45th Annual Meeting and ToxExpo
Place of Presentation
San Diego, California, USA
Year and Date
20060305-09
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Current status of maternal and fetal exposure to brominated flame retardants, PCBs and dioxins in Japan.2005
Author(s)
H. Fukata, M. Omori-Inoue, H. Osada, E. Todaka, N. Omori, Y. Kawashiro, S. Takahashi, H. Takigami, S. Sakai and C. Mori
Organizer
Dioxin 2005 and ISPAC-20
Place of Presentation
Toronto, Canada
Year and Date
20050821-26
Description
「研究成果報告書概要(和文)」より
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[Presentation] Current status of maternal and fetal exposure to brominated flame retardants, PCBs and dioxins in Japan2005
Author(s)
H. Fukata, M. Omori-Inoue, H. Osada, E. Todaka, N. Omori, Y. Kawashiro, S. Takahashi, H. Takigami, S. Sakai and C. Mori.
Organizer
Dioxin 2005 and ISPAC-20
Place of Presentation
Toronto, Canada
Year and Date
20050821-26
Description
「研究成果報告書概要(欧文)」より
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