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2006 Fiscal Year Final Research Report Summary

Xyloglucan for generating tensile stress to bend tree stem

Research Project

Project/Area Number 17380108
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産科学・木質工学
Research InstitutionKyoto University

Principal Investigator

HAYASHI Takahisa  Kyoto University, RISH, Associate professor (70231529)

Co-Investigator(Kenkyū-buntansha) BABA Keiichi  Kyoto UNIV, RISH, Assistant (20238223)
TAKEDA Takumi  Kyoto UNIV, RISH, Researcher (80423036)
YOSHIDA Masato  Nagoya UNIV, Graduate School of Bioagricultural Sciences School Agricultural Sciences Biosphere Resources Science, Associate professor (30242845)
TANIGUCHI Toru  林木育種センター, 育種部育種工学課, 室長 (00360470)
KAIDA Rumi  Japan Women's UNIV, Department of Science, Researcher (00398885)
Project Period (FY) 2005 – 2006
KeywordsG-layer / xyloglucan / cellulose / xyloglucan endotransglycosylase / gravitropical response / tension
Research Abstract

We produced transgenic poplar plants overexpressing several endoglycanases to reduce each specific polysaccharide in the cell wall, as the secondary xylem consists of primary and secondary wall layers. When placed horizontally, the basal regions of stems of transgenic poplars overexpressing xyloglucanase alone could not bend upward due to low strain in the tension side of the xylem. In the wild-type plants, xyloglucan was found in the inner surface of G-layers during multiple layering. In situ xyloglucan endotransglucosylase (XET) activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, began at the inner surface layers S1 and S2 and was retained throughout G-layer development, while the incorporation of xyloglucan heptasaccharide (XXXG) for wall loosening occurred in the primary wall of the expanding zone. We propose that the xyloglucan network is reinforced by XET to form a further connection between wall-bound and secreted xyloglucans in order to withstand the tensile stress created within the cellulose G-layer microfibrils.

  • Research Products

    (29 results)

All 2008 2007 2006 2005 Other

All Journal Article (28 results) Book (1 results)

  • [Journal Article] Astrategy for desert afforestation using plastid transformation technique for CO2 sequestration2008

    • Author(s)
      S. Okumura, M. Sawada, M. Shimamura, Y-W, Park, T. Hayashi, A. Yamashita, M. Hattori, H. Kanamoto, H. Takase, C. Miyake, K. Tomizawa
    • Journal Title

      J. Arid Land Stud

      Pages: 505-508

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose2007

    • Author(s)
      T. Konishi, T. Takeda, Y. Miyazaki, M. Ohnishi-Kameyama, T. Hayashi, M. A. O', Neill, T. Ishii
    • Journal Title

      Glycobiology 17

      Pages: 345-354

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increase in Xet activity in been2007

    • Author(s)
      AAlonso-Simon, PGarcia-Angulo, AE Encina, JM Alvarez, JL Acebes, T. Hayashi
    • Journal Title

      Planet 226

      Pages: 765-771

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Xyloglucan Endo-tranceglycosylase (XET) Functions in Gelatino Use Layers of Tension Wood Fibers in Poplar2007

    • Author(s)
      NNishikubo, TAwano, ABanasiak, VBourquintn, F. Ibawicztullin, RFunaba, HBrumer, TTTeeri, THayashi, Bsundberg, EJMellero, SE Patterson
    • Journal Title

      Plant Cell Physiol 48

      Pages: 843-855

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stractual organization and a standardized nomenclature for Plant endo-1, 4-β-glucanase of glycosyl hydrolase family 92007

    • Author(s)
      BR Urbanowicz, AB Bennett, E del Campillo, C Catala, T Hayashi, B Henrissat, H. Hofte, SJ McQueen-Mason, SE Patterson, 0. Shoseyov TT Teeri, JKC Rose
    • Journal Title

      Plant Physiology 144

      Pages: 1693-1696

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose2007

    • Author(s)
      T. Konishi, T. Takeda, Y. Miyazaki, M. Ohnishi-Kameyama, T. Hayashi. M. A. O'Neill, T. Ishii
    • Journal Title

      Glycobiology 17

      Pages: 345-354

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Xyloglucan Endo-tranceglycosylase(XET)2007

    • Author(s)
      NNishikubo, TAwano, ABanasiak, VBourquinta, F. Ibawicztullin. RFunaba, HBrumer, TTTeeri, THayashi, BSundberand EJMellero, SE Patterson
    • Journal Title

      Functions in GelatinoUse Layers of Tension Wood Fibers in Poplar Plant Cell Physiol 48

      Pages: 843-855

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stractual organization and a standardized nomenclature for Plant endo-1, 4-β-glucanase of glycosyl hydrolase family 92007

    • Author(s)
      BR Urbanowicz, AB Bennett, E del Campillo, C Catala, T Hayashi, B Henrissat, H. Hofte, S J McQueen-Mason, SE Patterson, O. Shoseyov TT Teeri, JKC Rose
    • Journal Title

      Plant Physiology 144

      Pages: 1693-1696

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Science and Lore of the Plant Cell Wall-Biosynthesis2006

    • Author(s)
      T. hayashi
    • Journal Title

      Structure and Function

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tree biotechnology in Indonesia2006

    • Author(s)
      Sudarmonowati, T. Hayashi
    • Journal Title

      Sustainable Development and utilization of tropicalforest resources

      Pages: 113-122

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tissue culture and molecular breeding of tropical trees2006

    • Author(s)
      T. Hayashi
    • Journal Title

      Sustanable Develpoment and utilization of tropical forest resources

      Pages: 189-190

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Does xyloglucan endotransglycosylase promote cell elongation?2006

    • Author(s)
      Takahisa Hayashi, Takumi Takeda
    • Journal Title

      The Science and Lore of the Plant Cell Wall

      Pages: 203-213

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Astrategy for desert afforestation using plastid transformation technique for CO2 sequestration2006

    • Author(s)
      S. Okumura, M. Sawada, M. Shimamura, Y-W. Park, T. Hayashi, A. Yamashita, M. Hattori, H. Kanamoto, H. Takase, C. Miyake, K. Tomizawa
    • Journal Title

      J. Arid Land Stud 15

      Pages: 505-508

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts2006

    • Author(s)
      S. Okumura, M. Sawada, Y-W, Park, T. Hayashi, M. Shimamura, H. Takase, K. Tomizawa
    • Journal Title

      Transgenic Res. 15

      Pages: 637-646

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Is cellulose synthesis enhanced by expression of sucrose synthase in poplar?2006

    • Author(s)
      T. Hayashi, T. Konishi, Y. Ohmiya, T. Nakai
    • Journal Title

      Abiotic Stress Tolerance in Plants

      Pages: 187-193

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tree biotechnology in Indonesia2006

    • Author(s)
      Sudannonowati, T. Hayashi
    • Journal Title

      Sustainable Development and utilization of tropical forest resources

      Pages: 113-122

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Does xyloglucan endotransglycosylase promote cell elongation ?2006

    • Author(s)
      TakahisaHayashi, Takumi Takeda
    • Journal Title

      The Science and Lore of the Plant Cell Wall

      Pages: 203-213

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transformation of poplar {Populus alba) plastids and expression of foreign proteins in tree chloroplasts2006

    • Author(s)
      S. Okmmura, M. Sawada, Y-W, Park, T. Hayashi, M. Shraiamura H. Takase, K. Tomizawa
    • Journal Title

      Transgenic Res 15

      Pages: 637-646

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Is cellulose synthesis enhanced by expression of sucrose synthase in poplar ?2006

    • Author(s)
      T. Hayashi, T. Konishi, Y. Ohmiya, T. Nakai
    • Journal Title

      Abiotic Stress Tolerance in Plants

      Pages: 187-193

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 糖鎖分解酵素の過剰発言によるセルロースとキシログルカンの機能解明2005

    • Author(s)
      林隆久, 馬場啓一
    • Journal Title

      植物成長調節 40

      Pages: 164-174

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cellulose metabolism in plants2005

    • Author(s)
      T. Hayashi, K. Yoshida, Y. W. Park, T. Konishi, K. Baba
    • Journal Title

      International Review of Cytology 247

      Pages: 1-34

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Callose Syndromes2005

    • Author(s)
      T. Hayashi
    • Journal Title

      Hwt : //www. glycoforum. gr. jp/science/word/glycbiology/PS-A03E. html

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of cellulose gene in Paraserianthes Falcataria2005

    • Author(s)
      EnnySudarmonowati, SriHartati, RetnaHartati, YongWooPark, TakahisaHayashi
    • Journal Title

      Towards Ecology and Economy Harmonization of Tropical Forest Resources

      Pages: 388-394

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Agrobacterium-mediated genetic transformation of Acaciamangium bearing xyloglucanase2005

    • Author(s)
      SriHartai, YongWooPark, E. Sudarmonwati, TakahisaHayashi
    • Journal Title

      Towards Ecology and Economy Harmonization of Tropical Forest Resources

      Pages: 395-399

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cellulose metabolism in plants2005

    • Author(s)
      T. Hayashi, K. Yoshida, Y. W. Park, T. Konishi a K. Baba
    • Journal Title

      International Review of Cytology 247

      Pages: 1-34

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 「研究成果報告書概要(欧文)」より2005

    • Author(s)
      T. Hayashi
    • Journal Title

      (CalloseSyndromesHwt://www.glycoforum.gr.jp/science 'word/glycbiology/PS-A03E.html)

  • [Journal Article] Agrobacterium-mediated genetic transformation of Acacia mangium bearing xyloglucanase2005

    • Author(s)
      SriHartai, YongWooPark, E.. Sudarmonwati, TakahisaHayashi
    • Journal Title

      Towards Ecology and Economy Harmonization of Tropical Forest Resources

      Pages: 395-399

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Analysis of cellulose arid xyloglucan function by carbohydrolaseoverexpression in transgenic plants

    • Author(s)
      T. Hayashi, K. Baba
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] The Science and Lore of the Plant Cell Wall-Biosynthesis, Structure and Function2006

    • Author(s)
      T. Hayashi
    • Total Pages
      355
    • Publisher
      Brown Walker Press
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2010-06-09   Modified: 2018-03-20  

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