2006 Fiscal Year Final Research Report Summary
Xyloglucan for generating tensile stress to bend tree stem
| Project/Area Number |
17380108
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産科学・木質工学
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| Research Institution | Kyoto University |
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Principal Investigator |
HAYASHI Takahisa Kyoto University, RISH, Associate professor (70231529)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Keiichi Kyoto UNIV, RISH, Assistant (20238223)
TAKEDA Takumi Kyoto UNIV, RISH, Researcher (80423036)
YOSHIDA Masato Nagoya UNIV, Graduate School of Bioagricultural Sciences School Agricultural Sciences Biosphere Resources Science, Associate professor (30242845)
TANIGUCHI Toru 林木育種センター, 育種部育種工学課, 室長 (00360470)
KAIDA Rumi Japan Women's UNIV, Department of Science, Researcher (00398885)
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| Project Period (FY) |
2005 – 2006
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| Keywords | G-layer / xyloglucan / cellulose / xyloglucan endotransglycosylase / gravitropical response / tension |
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| Research Abstract |
We produced transgenic poplar plants overexpressing several endoglycanases to reduce each specific polysaccharide in the cell wall, as the secondary xylem consists of primary and secondary wall layers. When placed horizontally, the basal regions of stems of transgenic poplars overexpressing xyloglucanase alone could not bend upward due to low strain in the tension side of the xylem. In the wild-type plants, xyloglucan was found in the inner surface of G-layers during multiple layering. In situ xyloglucan endotransglucosylase (XET) activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, began at the inner surface layers S1 and S2 and was retained throughout G-layer development, while the incorporation of xyloglucan heptasaccharide (XXXG) for wall loosening occurred in the primary wall of the expanding zone. We propose that the xyloglucan network is reinforced by XET to form a further connection between wall-bound and secreted xyloglucans in order to withstand the tensile stress created within the cellulose G-layer microfibrils.
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Research Products
(29 results)
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[Journal Article] Astrategy for desert afforestation using plastid transformation technique for CO2 sequestration2008
Author(s)
S. Okumura, M. Sawada, M. Shimamura, Y-W, Park, T. Hayashi, A. Yamashita, M. Hattori, H. Kanamoto, H. Takase, C. Miyake, K. Tomizawa
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Journal Title
J. Arid Land Stud
Pages: 505-508
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Xyloglucan Endo-tranceglycosylase (XET) Functions in Gelatino Use Layers of Tension Wood Fibers in Poplar2007
Author(s)
NNishikubo, TAwano, ABanasiak, VBourquintn, F. Ibawicztullin, RFunaba, HBrumer, TTTeeri, THayashi, Bsundberg, EJMellero, SE Patterson
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Journal Title
Plant Cell Physiol 48
Pages: 843-855
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Stractual organization and a standardized nomenclature for Plant endo-1, 4-β-glucanase of glycosyl hydrolase family 92007
Author(s)
BR Urbanowicz, AB Bennett, E del Campillo, C Catala, T Hayashi, B Henrissat, H. Hofte, SJ McQueen-Mason, SE Patterson, 0. Shoseyov TT Teeri, JKC Rose
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Journal Title
Plant Physiology 144
Pages: 1693-1696
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Xyloglucan Endo-tranceglycosylase(XET)2007
Author(s)
NNishikubo, TAwano, ABanasiak, VBourquinta, F. Ibawicztullin. RFunaba, HBrumer, TTTeeri, THayashi, BSundberand EJMellero, SE Patterson
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Journal Title
Functions in GelatinoUse Layers of Tension Wood Fibers in Poplar Plant Cell Physiol 48
Pages: 843-855
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Stractual organization and a standardized nomenclature for Plant endo-1, 4-β-glucanase of glycosyl hydrolase family 92007
Author(s)
BR Urbanowicz, AB Bennett, E del Campillo, C Catala, T Hayashi, B Henrissat, H. Hofte, S J McQueen-Mason, SE Patterson, O. Shoseyov TT Teeri, JKC Rose
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Journal Title
Plant Physiology 144
Pages: 1693-1696
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Astrategy for desert afforestation using plastid transformation technique for CO2 sequestration2006
Author(s)
S. Okumura, M. Sawada, M. Shimamura, Y-W. Park, T. Hayashi, A. Yamashita, M. Hattori, H. Kanamoto, H. Takase, C. Miyake, K. Tomizawa
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Journal Title
J. Arid Land Stud 15
Pages: 505-508
Description
「研究成果報告書概要(和文)」より
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