Identification of cell-type specific active sites from laminins and characterization of the functional mechanisms

2008 Fiscal Year Final Research Report

• Project/Area Number: 17390024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: NOMIZU Motoyoshi Tokyo University of Pharmacy and Life Science, 薬学部, 教授 (00311522)
• Project Period (FY): 2005 – 2008
• Keywords: 細胞・組織 / 生体分子 / 蛋白質 / 再生医学 / 薬学

Research Abstract

細胞外マトリックスは細胞接着をはじめ器官形成、神経網再生、血管新生やがんの増殖転移などの様々な生命現象や病態に深く関わっている。細胞外マトリックスの主役的な巨大分子であるラミニンの細胞接着活性部位を組換えタンパクと1000種類以上の合成ペプチドを用いて同定し、それぞれの活性部位の細胞膜上のレセプターや様々な生物活性を解析し、さらにはそれらのペプチドを用いた創薬や再生医療への応用研究を行った。

Research Products

• [Journal Article] Chain-specific heparin-binding sequences in the laminin・chain LG4-5 modules (2009)
  • Author(s): Hozumi K, Suzuki N, Uchiyama Y, Katagiri F, Kikkawa Y, Nomizu M
  • Journal Title: Biochemistry (印刷中)
  • Peer Reviewed
• [Journal Article] Clustering of syndecan-4 and integrin・1 by laminin・3 chain-derived peptide promotes keratinocyte migration (2009)
  • Author(s): Araki E, Momota Y, Togo T, Tanioka M, Hozumi K, Nomizu M, Miyachi Y, Utani A
  • Journal Title: Mol. Biol. Cell (印刷中)
  • Peer Reviewed
• [Journal Article] Peptide-chitosan matrix : a new multifunctional biomaterial (2009)
  • Author(s): Nomizu M, Yamagata N, Mochizuki M, Kikkawa Y, Kadoya Y
  • Journal Title: Adv. Exp. Med. Biol 611 Pages: 253-255
  • Peer Reviewed
• [Journal Article] Mixed peptide-chitosan membranes can mimic biological activities of a multifunctional laminin ・1 chain LG4 module (2009)
  • Author(s): Hozumi K, Yamagata N, Otagiri D, Fujimori C, Kikkawa Y, Kadoya Y, Nomizu M
  • Journal Title: Biomaterials 30 Pages: 1596-1603
  • Peer Reviewed
• [Journal Article] A novel cell adhesive scaffold material for delivering keratinocytes reduces granulation tissue in dermal wounds (2009)
  • Author(s): Masuda R, Mochizuki M, Hozumi K, Takeda A, Uchinuma E, Yamashina S, Nomizu M, Kadoya Y
  • Journal Title: Wound Repair Regen 17 Pages: 127-135
  • Peer Reviewed
• [Journal Article] Laminin alpha 5 mediates ectopic adhesion of hepatocellular carcinoma through integrins and/or Lutheran/basal cell adhesion molecule (2008)
  • Author(s): Kikkawa Y, Sudo R, Kon J, Mizuguchi T, Nomizu M, Hirata K, Mitaka T
  • Journal Title: Exp. Cell Res 314 Pages: 2579-2590
  • Peer Reviewed
• [Journal Article] The LG1-3 tandem of laminin ・5 harbors the binding sites of Lutheran/basal cell adhesion molecule and ・3・1/・6・1 integrins (2007)
  • Author(s): Kikkawa Y, Sasaki T, Nguyen MT, Nomizu M, Mitaka T, Miner JH
  • Journal Title: J. Biol. Chem 282 Pages: 14853-14860
  • Peer Reviewed
• [Journal Article] Identification of multiple amyloidogenic sequences in laminin-1 (2007)
  • Author(s): Kasai S, Urushibata S, Hozumi K, Yokoyama F, Ichikawa N, Kadoya Y, Nishi N, Watanabe N, Yamada Y, Nomizu M
  • Journal Title: Biochemistry 46 Pages: 3966-3974
  • Peer Reviewed
• [Journal Article] Integrin- dependent cell behavior on ECM peptide- conjugated chitosan membranes (2007)
  • Author(s): Mochizuki M, Yamagata N, Philp D, Hozumi K, Watanabe T, Kikkawa Y, Kadoya Y, Kleinman HK, Nomizu M
  • Journal Title: Biopolymers 88 Pages: 122-130
  • Peer Reviewed
• [Journal Article] Cyclic peptide analysis of the biologically active loop region in the laminin alpha 3 chain LG4 module (2007)
  • Author(s): Kato-Takagaki K, Suzuki N, Yokoyama F, Takaki S, Mochizuki M, Kikkawa Y, Oishi S, Utani A, Nomizu M
  • Journal Title: Biochemistry 46 Pages: 1952-1960
• [Journal Article] Angiogenic activitiy of syndecan-binding laminin peptide AG73 (RKRLQVQLSIRT) (2007)
  • Author(s): Mochizuki M, Philp D, Kleinman HK, Nomizu M
  • Journal Title: Arch. Biochem. Biophys 459 Pages: 249-255
  • Peer Reviewed
• [Journal Article] Laminin ・1 chain LG4 module promotes cell attachment through syndecans and cell spreading through integrin・2・1 (2006)
  • Author(s): Hozumi K, Suzuki N, Nielsen PK, Nomizu M, Yamada Y
  • Journal Title: J. Biol. Chem 281 Pages: 32929-32940
  • Peer Reviewed
• [Journal Article] Laminin peptide-conjugated chitosan membrane : a new system for cell delivery in wounded skin (2006)
  • Author(s): Ikemoto S, Mochizuki M, Yamada M, Takeda A, Uchinuma E, Yamashina S, Nomizu M, Kadoya Y
  • Journal Title: J. Biomed. Mater. Res. A 79 Pages: 716-722
  • Peer Reviewed
• [Journal Article] Laminin ・3 LG4 module induces keratinocyte migration : involvement of matrix metalloproteinase-9 (2005)
  • Author(s): Momota Y, Suzuki N, Kasuya Y, Kobayashi T, Mizoguchi M, Yokoyama F, Nomizu M, Shinkai H, Iwasaki T, Utani A
  • Journal Title: J. Recept. Signal Transduct. Res 25 Pages: 1-17
  • Peer Reviewed
• [Journal Article] Laminin alpha 5 chain metastasisi- and angiogenesisi- inhibiting peptide blocks FGF2 activity by binding to the heparan sulfate chains of CD44 (2005)
  • Author(s): Hibino S, Shibuya M, Hoffman MP, Engbring JA, Hossain R, Mochizuki M, Kudoh S, Nomizu M, Kleinman HK
  • Journal Title: Cancer Res 65 Pages: 10494-10501
  • Peer Reviewed
• [Journal Article] Bifunctional peptides derived from homologuos Loop regions in the laminin・・chain LG4 modules interact with both・2・1 integrin and syndecan-2 (2005)
  • Author(s): Yokoyama F, Suzuki N, Kadoya Y, Utani A, Nakatsuka H, Nishi N, Haruki M, Kleinman HK, Nomizu M
  • Journal Title: Biochemistry 44 Pages: 9581-9589
  • Peer Reviewed
• [Journal Article] Functional sites in the laminin alpha chains (2005)
  • Author(s): Suzuki N, Yokoyama F, Nomizu M
  • Journal Title: Connect. Tissue Res 46 Pages: 142-152
  • Peer Reviewed
• [Presentation] Cell adhesive peptides-conjugated chitosan membranes promote diverse biological activities (2008)
  • Author(s): Otagiri D, Fujimori C, Hozumi K, Kikkawa Y, Nomizu M
  • Organizer: The American Society for Cell Biology 48th Annual Meeting, San Francisco
  • Place of Presentation: CA, USA
  • Year and Date: 2008-12-13
• [Presentation] Identification of cell adhesive and neurite outgrowth promoting sequences in the laminin・2 chain G domain (2008)
  • Author(s): Urushibata S, Hayashi T, Kobayashi K, Hozumi K, Kikkawa Y, Nomizu M
  • Organizer: 4^<th> Korea-Japan Workshop on Combinatorial Bioengineering
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2008-11-22
• [Presentation] Cell ahhesive laminin-derived peptide-polysaccharide complexes (2008)
  • Author(s): Yamada Y, Hozumi K, Nomizu M
  • Organizer: 12^<th> Korean Petide-Protein Society Symposium Seoul
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2008-11-21
• [Presentation] 複数のペプチドを固定化した機能性キトサン膜 (2008)
  • Author(s): 藤森能、小田切大、保住建太郎、吉川大和、野水基義
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-24
• [Presentation] Cell adhesive laminin peptides for tissue engineering (2008)
  • Author(s): 野水基義、他5名
  • Organizer: 30^<th> European Peptide Symposium
  • Place of Presentation: Helsinki, Finland
  • Year and Date: 2008-08-31
• [Presentation] Peptide-chitosan membranes promote diverse biological functions (2008)
  • Author(s): 野水基義、他3名
  • Organizer: Gordon Research Conferences, Biddeford
  • Place of Presentation: ME, USA
  • Year and Date: 2008-07-22
• [Presentation] Cell adhesive peptide as a powerful tool for cell- and tissue-engineering (2008)
  • Author(s): 野水基義
  • Organizer: The 12^<th> Akabori Conference
  • Place of Presentation: 京都
  • Year and Date: 2008-05-15
• [Presentation] マトリックスタンパクの分子解剖と医薬応用 (2008)
  • Author(s): 野水基義
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-26
• [Presentation] Cell adhesive peptide as a powerful tool for cell engineering (2007)
  • Author(s): 野水基義、他4名
  • Organizer: 4^<th> International Peptide Symposium/7^<th> Australian Peptide Conference
  • Place of Presentation: Cairns, Queensland, Australia
  • Year and Date: 2007-12-21
• [Presentation] Peptide-chitosan membranes promote diverse biological functions, The American Society for Cell Biology (2007)
  • Author(s): 野水基義、他2名
  • Organizer: 47th Annual Meeting, Washington
  • Place of Presentation: DC, USA
  • Year and Date: 2007-12-01
• [Presentation] Cell adhesive peptide- chitosan membranes : a new multifunctional biomedical material (2007)
  • Author(s): 野水基義、他3名
  • Organizer: 11th Korean Peptide Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-11-30
• [Presentation] Identification of biologically active sites on the N-terminal domain of laminin alpha2chain (2007)
  • Author(s): Hayashi T, Hozumi K, Kikkawa Y, Nomizu M
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-10
• [Presentation] Cell adhesive peptide- chitosan matrix : a new multifunctional biomaterial (2007)
  • Author(s): 野水基義、他3名
  • Organizer: The 6^<th> Symposium on Frontiers in Protein Chemistry and Biotechnology
  • Place of Presentation: Changchun, China
  • Year and Date: 2007-08-22
• [Presentation] Peptide-chitosan matrix : a new multifunctional biomaterial (2007)
  • Author(s): 野水基義、他4名
  • Organizer: 20^<th> American Peptide Society Symposium
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2007-06-26
• [Presentation] An α-dystroglycan binding peptide derived from the laminin α2 chain (2006)
  • Author(s): 野水基義、他5名
  • Organizer: The American Society for Cell Biology 46^<th> Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2006-12-09
• [Presentation] Cell adhesive peptides as a powerful tool for biomedical materials (2006)
  • Author(s): 野水基義、他4名
  • Organizer: 11^<TH> Ababoori Conference
  • Place of Presentation: Kloster Banz, Germany
  • Year and Date: 2006-11-11
• [Presentation] Cell adhesive peptide- conjugated chitosan membrane : a new system for cell delivery (2006)
  • Author(s): 野水基義、他5名
  • Organizer: 第43回ペプチド討論会
  • Place of Presentation: 横浜
  • Year and Date: 2006-11-05
• [Presentation] Cell adhesive peptide- conjugated chitosan membrane : as a bio-medical basement membrane for tissue engineering (2006)
  • Author(s): 野水基義、他2名
  • Organizer: 29^<th> European Peptide Symposium
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2006-11-03
• [Presentation] 多機能性ペプチド-キトサン膜の創製 (2006)
  • Author(s): 山縣夏美、望月麻友美、吉川大和、山田真路、門谷裕一、野水基義
  • Organizer: 第55回高分子討論会
  • Place of Presentation: 富山
  • Year and Date: 2006-09-20
• [Presentation] Characterization of・-dystroglycan binding sequence in the laminin・2 chain LG45 module (2006)
  • Author(s): 野水基義、他5名
  • Organizer: Research Conferences
  • Place of Presentation: Barga, Italy
  • Year and Date: 2006-06-18