2007 Fiscal Year Final Research Report Summary
OXIDATIVE STRESS BY SE-DEFICIENCY AND DYNAMICS OF BIOTRACE ELEMENTS
| Project/Area Number |
17590040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Showa Pharmaceutical University |
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Principal Investigator |
ENDO Kazutoyo Showa Pharmaceutical University, DEPT. OF PHARMACY, PROFESSOR (50106598)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Chikako SHOWA PHARMACUETICAL UNIVERSITY, DEPT. OF PHARMACY, Associate Professor (00199572)
MATSUOKA Keisuke SHOWA PHARMACUETICAL UNIVERSITY, DEPT. OF PHARMACY, RESEARCH ASSOCIATE (90384635)
ENOMOTO Shuichi RIKEN, 加速器基盤研究部, REASERCH FELLOW (10271553)
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| Project Period (FY) |
2005 – 2007
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| Keywords | TRECE EMEMENT / SE-DEFICIENCY / OXIDATIVE STRESS / ESR / GSH-Px / RATS / ROS |
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| Research Abstract |
1) In vivo free radical reactions in rat liver as a result of exposure to low-dose β-radiation was evaluated with electron paramagnetic resonance (EPR) spectroscopy by monitoring the reduction of the nitroxyl spin probe after intravenous administration. Results suggest that under low levels of tissue oxygen, exposure to β-rays results in nitroxyl signal loss, which may be mediated by free radical dependent pathways.
2) Biochemical data of AST, ALT, and BUN for the plasma fraction of the rat bloods, and the concentration of vitamin C and vitamin E were measured in the liver homogenates. The relationships among the biotrace elements and the biochemical data were compared, and the indices for oxidative stress caused by selenium deficiency were discussed in terms of reactive oxygen species (ROS) , and antioxidants in the organism.
3) Free radical-reducing activity and membrane fluidity of liver microsomes from Se-deficient (SeD) Wistar rats were examined by EPR spin label method using nitrox
… More
yl-labeled stearic acids. SeD induced the liver microsomal cytochrome P 450 activity ; the nitroxyl spin probes are substrates for reduction-relating cytochrome P 450. The reduction rate of nitroxyl radical at shallow depth in membranes was increased.
4) Although the oxidative stress caused Fe accumulation in the liver, the Fe concentration in bile of the SeD rat was almost the same as that in the control rats. Due to the trapping of reactive oxygen species, only bilirubin concentrations in the bile and plasma were decreased in the SeD group, when compared with those in the control group. Measurement of bilirubin concentration may be used as a supplemental oxidative stress marker.
5) Dynamics of redox relating biotrace elements, selenium, iron, and zinc in liver, kidney, and spleen of selenium deficient Wistar male rats in a series of feeding period (from 0 to 8 weeks) were studied using instrumental neutron activation analysis. The results were compared with those of aspartate aminotransferase (AST) , alanine aminotransferase (ALT) , and blood urea nitrogen (BUN) for the plasma fraction of the rat bloods. Less
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[Journal Article] Changes in the components of biliary and plasma lipids in selenium-deficient rats2007
Author(s)
Yasunobu, Sakuma, Junya Sasaki, Aya, Futami, Kousuke, Yamasaki, Keisuke, Matsuoka, Chikako, Honda, Kazutoyo, Endo, Masamichi, Tsukada
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Journal Title
Chem. Phys. Lipids 148(2)
Pages: 70-76
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Feeding period of selenium-deficient diet and response of redox relating minerals2006
Author(s)
Matsumoto, Ken-ichiro;Ariyoshi, Marie;Terada, Sachiyo;Okajo, Aya;Urata, Hiromi;Sakuma, Yasumobu;Satoh, Kazue;Ushio, Fusao;Tsukada, Masamichi;Endo, Kazutoyo
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Journal Title
J.Health Scie 52
Pages: 694-702
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Feeding period of selenium-deficient diet and response of redox relating minerals2006
Author(s)
Matsumoto, Ken-ichiro, Ariyoshi, Marie, Terada, Sachiyo, Okajo, Aya, Urata, Hiromi, Salcuma, Yasunobu, Satoh, Kazue, Ushio, Fusao, Tsukada, Masamichi, Endo, Kazutoyo
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Journal Title
J. Health Sci 52(6)
Pages: 694-702
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Electron paramagnetic resonance decay constant and oxidative stresses in liver microsomes of the selenium-deficient rat2006
Author(s)
Matsumoto, Ken-ichiro, Suzuki, Ayako, Washimi, Haruka, Hisamatsu, Atsushi, Okajo, Aya, Ui, Iori, Endo, Kazutoyo
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Journal Title
J.Nutr. Biochem 17(10)
Pages: 677-681
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Estimation of free radical formation by β-ray irradiation in rat liver2005
Author(s)
Matsumoto, Ken-ichiro, Okajo, Aya, Kobayashi, Takenori, Mitchell, James B., Krishna, Murali, C., Endo, kazutoyo
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Journal Title
J.Biochem.Biophys.Meth 63(2)
Pages: 79-90
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Correlation between Ketone body level in selenium-deficient rats and oxidative damages2005
Author(s)
Matsumoto, Ken-ichiro, Takuwa, Aid, Takenori, Akemi, Ui, Iori, Okajo, Aya, Endo, Kazutoyo
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Journal Title
Biol.Pharm.Bull 28(7)
Pages: 1142-1147
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Dynamics of redox related elements(Fe, Co, Zn and Se) and oxidative stress caused by Se-deficiency in rats2007
Author(s)
Y., Sakuma, K., Matsuoka, C., Honda, K., Matsumoto, K., Endo
Organizer
12 th Int. Conf.'Moden trends activation analysis(MTAA-12) Hachiohji
Year and Date
20070916-18
Description
「研究成果報告書概要(欧文)」より
