2007 Fiscal Year Final Research Report Summary
Mechanistic analysis for androgen independent growth of prostate cancers
| Project/Area Number |
17590356
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Nagoya City University |
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Principal Investigator |
ASAMOTO Makoto Nagoya City University, Graduate School of Medical Science, Associate Professor (50212494)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Prostate cancer / cell culture / androgen / steroid hormone / cancer stem cells |
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| Research Abstract |
We generated a transgenic rat with the SV40 T antigen under probasin promoter control, allowing prostate-specific gene expressions. Males demonstrate atypical epithelial cell proliferation in the prostate from 4 weeks of age and develop prostate carcinomas at 100% incidence before they are 15 weeks old. From the transgenic rat of 55 week-old, we established a transplanted tumor in nude mice with castration treatments. And then, we have tried to established cell lines from the nude mice tumor. Finally we established 3 cell lines. One cell line designated PCai-1 shows charcoal treated FBS insensitive growth, and two other cell lines PCai-2 and PCai-3 are sensitive for the charcoal treatment FBS with stopping growth. Characteristic gene expression profiles have been obtained for each 3 cell lines. These cell lines may be useful to study mechanisms of progression of prostate cancers, and to study chemo-prevention of prostate cancers. Furthermore, we established new cell lines from the non-castrated androgen dependent transplanted tumors. These cells had the stem cell like characters such as asymmetric tumor growth and CD133 expression.
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[Journal Article] Glutathione S-transferase pi mediates proliferation of androgen independent prostate cancer cells2008
Author(s)
Hokaiwado, N., Takeshita, F., Naiki-Ito, A., Asamoto, M., Ochiya, T., Shirai, T
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Journal Title
Carcinogenesis., Epub ahead of print
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Protective effects of citrus nobiletin and auraptene in transgenic rats developing adenocarcinoma of the prostate (TRAP) and human prostate carcinoma cells2007
Author(s)
Tang, M., Ogawa, K., Asamoto, M., Hokaiwado, N., Azman, S., Suzuki, S., Takahashi, S., Tanaka, T., Ichikawa, K., Shirai, T
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Journal Title
Cancer Sc 98
Pages: 471-477
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization and application of polycolonal antibodies that specifically recognize JC virus large T antigen2006
Author(s)
Sunden, Y., Suzuki, T., Orba, Y., Umemura, T., Asamoto, M., Nagashima, K., Tanaka, S., Sawa, H
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Journal Title
Acta. Neuropathol 111
Pages: 379-387
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibition of prostate carcinogenesis in probasin/SV40 T antigen transgenic rats by leuprorelin, a luteinizing hormone-releasing hormone agonist2006
Author(s)
Said, M., Hokaiwado, N., Tang, M., Ogawa, K., Suzuki, S., Ghanem, H. N., Esmat, A. Y., Asamoto, M., Fawzia, M. R., Shirai, T
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Journal Title
Cancer Sci 97
Pages: 459-467
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Close relation of Transforming Growth Factor a positive hepatic foci to hepatocellular tumor development in the rat2006
Author(s)
Tamano, S., Kawabe, M., Ichihara, T., Hagiwara, A, Sano, M., Asamoto, M., Shirai, T
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Journal Title
J Toxicol Pathol 19
Pages: 99-106
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prediction of carcinogenic potential by a toxicogenomic approach using rat hepatoma cells2006
Author(s)
Tsujimura, K., Asamoto, M., Shugo, S., Hokaiwado, N., Ogawa, K., Shirai, T
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Journal Title
Cancer Sci 97
Pages: 1002-1010
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Harman and norharman suppressed but NaNO2 enhanced the development of preneoplastic liver cell foci in 2-amino-3, 8-dimethylimidazo[4,5-f] quinoxaline (MeIQx)-reated rats2005
Author(s)
Sugiura, S., Asamoto, M., Hokaiwado, N., Hirose, M., Shirai, T
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Journal Title
J., Toxicol. Pathol 18
Pages: 99-104
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Equivocal impact of transplacental and lactational exposure to a food-derived carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-3]pyridine, on prostate and colon lesion development in F344 rats2005
Author(s)
Ikeda, Y., Cho, Y. M., Takahashi, S., Tang, M., Asamoto, M., Ogawa, K., Shirai, T
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Journal Title
Cancer Lett 224
Pages: 23-30
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Influence of atrazine administration and reduction of calorie intake on prostate carcinogenesis in probasin/SV4OT antigen transgenic rats2005
Author(s)
Kandori, H., Suzuki, S., Asamoto, M., Murasaki, T., Tang, M., Ogawa, K., Shirai, T
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Journal Title
Cancer Sci 96
Pages: 221-226
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Lack of effects of 1439 MHz electromagnetic near field exposure on the blood-brain barrier in immature and young rats2005
Author(s)
Kuribayashi, M., Wang, J., Fujiwara, O., Doi, Y., Nabae, K., Tamano, S., Ogiso, T., Asamoto, M., Shirai, T
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Journal Title
Bioelectromagnetics 26
Pages: 578-588
Description
「研究成果報告書概要(欧文)」より
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