Functional analysis of a new identified squamous cell epithelium secreting cytokine.

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17591428
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KIKUCHI Shojiro Kyoto Prefectural University of Medicine, Graduate School of Medicine, assistant, 医学研究科, 助教(学内講師) (70381960)
• Co-Investigator(Kenkyū-buntansha): OCHIAI Toshiya Kyoto Prefectural University of Medicine, Graduate School of Medicine, assistant, 医学研究科, 助教(学内講師) (30311913) ; YAMAGISHI Hisakazu Kyoto Prefectural University of Medicine, Graduate School of Medicine, president, 医学研究科, 学長 (40128723)
• Project Period (FY): 2005 – 2006
• Keywords: carcinoma / development / differentiation / cell / tissue

Research Abstract

1. Clinical sample from cancer patient-Twenty samples of surgical specimens from esophageal cancer patient were successfully collected for RNA analysis and primary culture.
2. Dermokine expression assay---Real-time PCR assay show that expression levels of dermokine-beta were low in cancer tissue.
3. Primary culture of resected esophageal cancer tissue---We add recombinant dermokine-beta to cultured primary cell to clarify the function of dermokine-beta in squamous cell carcinoma cell.
4. Large scale purification of dermokine-alpha, beta---We successfully established 293EBNA-1 cell expression system to generate recombinant dermokine-beta protein. Large scale purification of dermokine-alpha were not successful.
5. Variant isoform of dermokine-We analyzed morphological change of esophageal cancer cell under various concentration of recombinant dermokine-beta, but we could not find obvious changes. Recently various isoform of dermokine were reported in other group. Now we focused on isoform specific function of dermokine in cancer cell.