2006 Fiscal Year Final Research Report Summary
Development, Analyze and Clinical Use of Tissue Engineering Large Diameter Autografts
| Project/Area Number |
17591490
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
YAMAMOTO Noboru Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (50297470)
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| Co-Investigator(Kenkyū-buntansha) |
SHIN'OKA Toshiharu Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20192122)
KUROSAWA Hiromi Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (50075511)
MATSUMURA Goki Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (20297469)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Regenerative Medicine / Tissue Engineering / Tissue Engineered graft / Biodegradable Scaffold / Bone Marrow Cell / long term result / Endothelial Cell / growth factor |
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| Research Abstract |
This study demonstrates the evaluation of tissue-engineered vascular autografts (TEVAs) constructed with autologous mononuclear-bone marrow cells (MN-BMCs) and a biodegradable scaffold using a canine inferior vena cava (IVC) model.
MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(1-lactide-co-ε-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. TEVAs were analyzed biochemically, biomechanically and histologically after implantation. When TEVAs were explanted and stimulated with acetylcholine at 1 month, they dose dependently produced nitrates and nitrites. N^G-nitro-L-arginine methylester significantly inhibited these reactions. On the stimulation by acetylcholine, factor VIII-positive cells of TEVAs produced eNOS proteins, and the ratio of eNOS/s17 mRNA were similar among native IVC, 1-and 3-months TEVAs. TEVAs had similar biochemical properties and wall thickness as native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification.
These results indicate that TEVAs are biocompatible material with functional endothelial cells and biomechanical properties without unwanted side-effects.
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