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2006 Fiscal Year Final Research Report Summary

Development, Analyze and Clinical Use of Tissue Engineering Large Diameter Autografts

Research Project

Project/Area Number 17591490
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionTokyo Women's Medical University

Principal Investigator

YAMAMOTO Noboru  Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (50297470)

Co-Investigator(Kenkyū-buntansha) SHIN'OKA Toshiharu  Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20192122)
KUROSAWA Hiromi  Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (50075511)
MATSUMURA Goki  Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (20297469)
Project Period (FY) 2005 – 2006
KeywordsRegenerative Medicine / Tissue Engineering / Tissue Engineered graft / Biodegradable Scaffold / Bone Marrow Cell / long term result / Endothelial Cell / growth factor
Research Abstract

This study demonstrates the evaluation of tissue-engineered vascular autografts (TEVAs) constructed with autologous mononuclear-bone marrow cells (MN-BMCs) and a biodegradable scaffold using a canine inferior vena cava (IVC) model.
MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(1-lactide-co-ε-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. TEVAs were analyzed biochemically, biomechanically and histologically after implantation. When TEVAs were explanted and stimulated with acetylcholine at 1 month, they dose dependently produced nitrates and nitrites. N^G-nitro-L-arginine methylester significantly inhibited these reactions. On the stimulation by acetylcholine, factor VIII-positive cells of TEVAs produced eNOS proteins, and the ratio of eNOS/s17 mRNA were similar among native IVC, 1-and 3-months TEVAs. TEVAs had similar biochemical properties and wall thickness as native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification.
These results indicate that TEVAs are biocompatible material with functional endothelial cells and biomechanical properties without unwanted side-effects.

  • Research Products

    (9 results)

All 2006 2005

All Journal Article (9 results)

  • [Journal Article] Evaluation of Tissue-Engineered Vascular Autografts2006

    • Author(s)
      Matsumura Goki
    • Journal Title

      Tissue Engineering 12(11)

      Pages: 3075

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 組織工学による心臓弁、血管再生2006

    • Author(s)
      宮本 真嘉
    • Journal Title

      医学のあゆみ 217(5)

      Pages: 424

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 先天性心疾患領域における再生医療・Tissue Engineeringの展望2006

    • Author(s)
      市原 有起
    • Journal Title

      小児外科 38(2)

      Pages: 239

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 培養人工血管-Tissue Engineering Graft-2006

    • Author(s)
      保々 恭子
    • Journal Title

      分子心血管病 7(5)

      Pages: 503

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Evaluation of Tissue-Engineered Vascular Autografts2006

    • Author(s)
      Goki Matsumura
    • Journal Title

      Tissue Engineering 12(11)

      Pages: 3075-

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005

    • Author(s)
      Shin' oka Toshiharu
    • Journal Title

      J Thorac Cardiovasc Surg 129(6)

      Pages: 1330-1338

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Hibino Narutoshi
    • Journal Title

      J Thorac Cardiovasc Surg 129(5)

      Pages: 1064-1070

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005

    • Author(s)
      Toshiharu Shin' oka
    • Journal Title

      J Thorac Cardiovasc Surg 129(6)

      Pages: 1330-8

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005

    • Author(s)
      Narutoshi Hibino
    • Journal Title

      J Thorac Cardiovasc Surg 129(5)

      Pages: 1064-70

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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