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2007 Fiscal Year Final Research Report Summary

Analysis of inner ear specific autoantibody in experimental autoimmune labyrinthitis model

Research Project

Project/Area Number 17591803
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionNippon Medical School

Principal Investigator

TOMIYAMA Shunichi  Nippon Medical School, Otorhinolaryngology, Professor (00094665)

Co-Investigator(Kenkyū-buntansha) WATANABE Kenichi  Nippon Medical School, Otorhinolaryngology, Lecturer (80281434)
Project Period (FY) 2005 – 2007
Keywordsinner ear autoimmune disease / inner ear specific autoantibody / animal model / SDS-PAGE / whole gel eluter / western blotting / proteome profiling / inner ear specific auto-antigen
Research Abstract

In order to establish experimental inner ear specific autoimmune labyrinthitis in mice, we designed to recover inner ear specific proteins from crude inner ear proteins by SDS-technique and proteome profiling. Thirty partitioned inner ear proteins according to molecular size were harvested through elution of crude inner ear antigens on Whole Gel Eluter. Serum from mice sensitized with 19 out of 30 partitions produced antibodies against bovine inner ear proteins by western blotting and 7 positive different bands were recognized. Proteome profiling of several partitions that demonstrated clear positive bands were selectively carried out. Partition number 3, 10 and 11 hit only COCH protein. Partition number 18, 22 and 24 hit non specific inner ear proteins. This study succeeded in isolation of inner ear specific proteins, suggesting that development of inner ear specific autoimmune animal model is possible to elucidate pathogenesis of autoimmune inner ear disease.

  • Research Products

    (18 results)

All 2008 2007 2006 2005

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (10 results)

  • [Journal Article] プロテオーム解析に基づく内耳特異的蛋白抗原の分離回収2008

    • Author(s)
      富山 俊一
    • Journal Title

      Otol Jpn 18

      Pages: 105-112

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 内耳免疫現象によるめまい発症について2008

    • Author(s)
      富山 俊一
    • Journal Title

      Equilibrium Res 67

      Pages: 242-250

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Recovery of inner ear specific protein based on proteome profiling2008

    • Author(s)
      Shunichi, Toraiyatna
    • Journal Title

      Otol Jpn 18-2

      Pages: 105-112

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunological pathogenesis in vertigo attack2008

    • Author(s)
      Shunichi, Tomiyama
    • Journal Title

      Equilibrium Res 67-3

      Pages: 242-250

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 低音障害型感音難聴-内耳自己抗体陽性一症例の治療経過-2007

    • Author(s)
      富山 俊一
    • Journal Title

      Otol Jpn 17

      Pages: 26-32

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A case of low tone sensory nerve hearing loss with positive inner ear autoantiboclies2007

    • Author(s)
      Shunichi, Tomiyama, Akihiko, Saito, Manbu, Nonaka
    • Journal Title

      Otol Jpn 17-1

      Pages: 26-32

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of cochlin in the vestibular organ of rats2005

    • Author(s)
      Ikezono T
    • Journal Title

      ORL J Otolarhinolaryngol Relat Spec 67

      Pages: 252-258

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression of cochline in the vestibular organ of rats2005

    • Author(s)
      Tetsuo, Ikezono, Susumu, Shindo, Masamichi, Ishizaki, Lishu, Li, Shuncihi, Tomiyama, Masaya, Takumida, Ruby, Pawankar, Atsushi, Watanabe, Akihiko Saito, Thshiaki, Yagi
    • Journal Title

      ORL 67

      Pages: 252-258

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Immunological pathogenesis in vertigo attack2007

    • Author(s)
      Shunichi, Tomiyama
    • Organizer
      66th Japan society for equilibrium research
    • Year and Date
      20071100
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Clinical experience of Autoimmune inner ear disease2007

    • Author(s)
      Shunichi, Tomiyama, Akihiko, Saito, Nozomu, Ogiwara, Chika, Ozu, Manabu, Nonaka, Mayumi, Kunitomo
    • Organizer
      17th Otological society of Japan
    • Year and Date
      20071000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Inner ear autoimmune injured pathogensis in acute low tone sensory nerve heraring loss2007

    • Author(s)
      Shunichi, Tomiyama, Akihiko, Saito, Chika, Ozu, Manabu, Nonaka, Atuko, Sakanushi, kenichi, Shimada
    • Organizer
      108th the Otorhinolaryngology society of Japan
    • Year and Date
      20070500
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 68kDa inner ear autoantibody in acute low tone sensory nerve hearing loss2007

    • Author(s)
      Shunichi, Tomiyama
    • Organizer
      25th Japan society of Immunology & allergology in Otorhinolaryngology
    • Year and Date
      20070300
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 内耳免疫現象によるめまい発症について2007

    • Author(s)
      富山 俊一
    • Organizer
      日本平衡医学会
    • Place of Presentation
      大阪
    • Year and Date
      2007-11-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 内耳自己免疫病の治療経験2007

    • Author(s)
      富山 俊一
    • Organizer
      第17回日本耳科学会
    • Place of Presentation
      福岡
    • Year and Date
      2007-10-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 急性低音障害型感音難聴の内耳自己免疫傷害病因2007

    • Author(s)
      富山 俊一
    • Organizer
      第108回日本耳鼻咽喉科学会
    • Place of Presentation
      金沢
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 急性低音傷害型感音難聴における68kDa内耳自己抗体の関与2007

    • Author(s)
      富山 俊一
    • Organizer
      第25回日本耳鼻咽喉科免疫アレルギー学会
    • Place of Presentation
      甲府
    • Year and Date
      2007-03-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Autoimmune pathogenesis of recurrent acute low tone sensory nerve hearing loss2006

    • Author(s)
      Shunichi, Tomiyama
    • Organizer
      16th Otological society of Japan
    • Year and Date
      20061000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 自己免疫病因を示唆した反復する急性低音傷害型感音難聴2006

    • Author(s)
      富山 俊一
    • Organizer
      第16回日本耳科学会
    • Place of Presentation
      青森
    • Year and Date
      2006-10-20
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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