Association of MMP-1 promoter polymorphism with risk of apical periodontitis.

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 17591997
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Kagoshima University
• Principal Investigator: OYAMA Tohru Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor (60233623)
• Co-Investigator(Kenkyū-buntansha): SUWA Motoko Kagoshima university, Medical and Dental Hospital, Assistant Professor (80206599) ; TOKUDA Masayuki Kagoshima university, Medical and Dental Hospital, Senior Assistant Professor (20253891)
• Project Period (FY): 2005 – 2007
• Keywords: Apical periodontitis / MMP-1 / Polymorphism / Diaffnosis of risk on apical lesion / Ets-1

Research Abstract

Transcription factor Ets-1 play critical role in MMP-1 mRNA expression. A single nucleotide polymorphism in the promoter region of -1607 by of 1VIMP-1 have influence on Ets-1 binding activity. To clarify the ability of Ets-1 binding activity, we examined the following experiment.
Methods : Human periodontal ligament cells were plated in cell culture dishes in α-MEM supplemented with 10% FBS. The confluent-stage cells were incubated with 1% FBS containing α-MEM for 24h, followed by treatment with 10 ng/ml TNF-α. After incubation, the culture supernatants and sediments were collected and analyzed by ELISA, Northern blot, and gel shift mobility assay.
Results TNF-α induced MMP-1 mRNA and protein in various HPDL cells, and there were variations slightly in its expression. However, there were no significant differences in analysis of protein production by ELISA. TNF-α-induced ets-1 mRNA and protein also did not differ among HPDL cells, and Ets-lactivation in response to TNF-α showed that there were variations in activations. We are undertaking further investigations in our laboratory to elucidate the precise genetic analysis of polymorphism involved in variations of MMP-1 expression by TNF-α stimulations.

Research Products

• [Journal Article] Runx2 is involved in the inhibition of MMP-13 expression by Roxitluomycin in human gingival epithelial cell cultures (2008)
  • Author(s): Tabuchi S, Sakuya T, Oyama T, Tokuda M, Tatsuyama S, Kajihara T, Nagaoka S, Beppu M, Sugihara K, Ikebe T, Shira sung K, Torii M
  • Journal Title: J Periodont Res
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Roxithromycin inhibits tumor necrosis factor-α-induced matrix metallo proteinase-l expression through regulating mitogen-activated protein kinase phosphorylation and Ets-1 expression (2007)
  • Author(s): Oyama T, Matsushita K, Sakuta T, Tokuda M, Tatsuyama S, Nagaoka S, Torii M
  • Journal Title: J Periodont Res 42 Pages: 53-61
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Roxithromycin inhibits tumor necrosis factor-a-induced matrix metalloproteinase-1 expression through regulating mitogen-activated protein kinase phosphorylation and Ets-1 expression. (2007)
  • Author(s): Oyama, T., Matsushita, K., Sakuta, T., Tokuda, M., Tatsuyama, S., Nagaoka, S., Torii, M.
  • Journal Title: J Periodont Res, Peer reviewed 42 Pages: 53-61
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Runx2 is involved in the inhibition of MMP-13 expression by Roxithromycin in human gingival epithelial cell cultures.
  • Author(s): Tabuchi, S., Sakuya, T., Oyama, T., Tokuda, M., Tatsuyama, S., Kajihara, T., Nagaoka, S., Beppu, M., Sugihara, K., Ikebe, T., Shirasuna, K., Torii, M.
  • Journal Title: J Periodont Res, Peer reviewed (in press)
  • Description: 「研究成果報告書概要(欧文)」より