2006 Fiscal Year Final Research Report Summary

Molecular Mechanism of invasion in Oral squamous cell carcinomas,-small GTPase regulates the invasion.

Research Project

Project/Area Number	17592075
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Surgical dentistry
Research Institution	Osaka University
Principal Investigator	NAKAHARA Hirokazu Osaka University, Dental Hospital, Associate Professor, 歯学部附属病院, 助教授 (70324796)
Co-Investigator(Kenkyū-buntansha)	KOGO Mikihiko Osaka University, Graduate School of Dentistry Hospital, Professor, 歯学研究科, 教授 (20205371)
Project Period (FY)	2005 – 2006
Keywords	cancer / invasion / metastasis / small GTPase
Research Abstract	Epithelial-mesenchymal interaction is crucial in invade surrounding tissues by metastatic tumor cells. Here, we presented modified an in vitro fibronectin degradation/invasion assay that was co-cultured tumor cell with fibroblast. Using this assay, we estimated invasive activity as to three cell lines derived from human oral squamous cell carcinomas with different invasion mode. According to Yamamoto Kohama's classification, OSC20 was classified into grade 3,OSC19 into grade 4C and HOC313 into grade 4D. HOC313 showed strong invasiveness in co-cultured with fibroblast than OSC20 and OSC19 did. Also, knock down for MT1-MMP of cancer cell or/and fibroblast completed inhibit in vitro invasiveness.