• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

The study on the effect that a reduction of insulin-sensitive hormone causes periodontal disease in diabetes patients

Research Project

Project/Area Number 17592184
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YAMAGUCHI Noboru  Kyushu University, Faculty of Dental Science, Preventive Dentistry, Research Associate, 歯学研究院, 助手 (00230368)

Project Period (FY) 2005 – 2006
Keywordsdiabetes / periodontal disease / periodontopathic bacteria / osteoclast / adiponectin / RANKL / NF-KB / nitric oxide
Research Abstract

Our previous epidemiologic studies suggested that periodontal disease is closely related to obesity and glucose tolerance. Since the level of adiponectin, an adipocyte-derived cytokine, in plasma had been reported to decrease in obese and type 2 diabetes patients, we explored the role of adiponectin in the etiology of periodontitis by using the D clone of RAW 264, which clone exhibits highly efficient osteoclast formation, to determine whether adiponectin acts as a regulatory molecule in osteoclast formation stimulated by lipopolysaccharide (LPS) of periodontopathic bacteria.
We observed that adiponectin acted as a potent inhibitor of osteoclast formation stimulated by Toll-like receptor 4 (TLR4) ligand and receptor activator of NF-κB ligand (RANKL). Since NF-κB is an important transcriptional factor in osteoclast formation, we examined the effect of the adiponectin on its transcriptional activity. Our luciferase assay showed that adiponectin was able to inhibit the TLR4-mediated NF-κB activity in RAW 264 cells. In addition, we observed that the cytokine was actually able to inhibit TLR4-mediated expression of the gene for inducible nitric oxide synthase and production of nitric oxide in the cells. These observations strongly suggest that adiponectin may function as a negative regulator of LPS/RANKL-mediated osteoclast formation in periodontal disease.

  • Research Products

    (12 results)

All 2007 2006 2005

All Journal Article (12 results)

  • [Journal Article] Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans2007

    • Author(s)
      Yamaguchi, N., et al.
    • Journal Title

      FEMS Immunology and Medical Microbiology 49・1

      Pages: 28-34

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Adiponectin inhibits osteoclast formation stimulated by lipopolysaccharide from Actinobacillus actinomycetemcomitans2007

    • Author(s)
      Yamaguchi, N., et al.
    • Journal Title

      FEMS Immunology and Medical Microbiology 49(1)

      Pages: 28-34

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of Porphyromonas gingivalis GroEL signaling via binding to Toll-like receptor 2 and 42006

    • Author(s)
      Martinez Argueta, J.G., Shiota, S., Yamaguchi, N., et al.
    • Journal Title

      Oral Microbiology and Immunology 21・4

      Pages: 245-251

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Induction of Porphyromonas gingivalis GroEL signaling via binding to Toll-like receptor 2 and 42006

    • Author(s)
      Martinez Argueta, J.G., Shiota, S., Yamaguchi, N., et al.
    • Journal Title

      Oral Microbiology and Immunology 21(4)

      Pages: 245-251

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Adiponectin inhibits Toll-like receptor family-induced signaling2005

    • Author(s)
      Yamaguchi, N., et al.
    • Journal Title

      FEBS Letters 579・30

      Pages: 6821-6826

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification and characterization of novel isoforms of human DP-1 : DP-lalpha regulates the transcriptional activity of E2F1 as well as cell cycle progression in a dominant-negative manner2005

    • Author(s)
      Ishida, H., Masuhiro, Y., Fukushima, A., Martinetz Argueta, J.G., Yamaguchi, N., et al.
    • Journal Title

      Journal of Biological Chemistry 280・26

      Pages: 24642-24648

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 糖尿病患者における口腔内ケアの重要性2005

    • Author(s)
      山口 登, 山下喜久
    • Journal Title

      日本歯科評論 65・5

      Pages: 171-173

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ヒト血漿レプチン濃度と甘味感受性との相関2005

    • Author(s)
      中村由紀, 太田理恵, 山口登ら
    • Journal Title

      日本味と匂学会誌 12・3

      Pages: 247-250

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Adiponectin inhibits Toll-like receptor family-induced signaling2005

    • Author(s)
      Yamaguchi, N., et al.
    • Journal Title

      FEBS Letters 579(30)

      Pages: 6821-6826

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification and characterization of novel isoforms of human DP-1 : DP-lalpha regulates the transcriptional activity of E2F1 as well as cell cycle progression in a dominant-negative manner2005

    • Author(s)
      Ishida, H., Masuhiro, Y., Fukushima, A., Martinetz Argueta, J.G., Yamaguchi, N., et al.
    • Journal Title

      Journal of Biological Chemistry 280(26)

      Pages: 24642-24648

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Importance of oral health care in diabetes patients2005

    • Author(s)
      Yamaguchi, N., Yamashita, Y.
    • Journal Title

      The NIPPON Dental Review 65(5)

      Pages: 171-173

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Correlation between the leptin level in human plasma and the sensibility to sweet taste2005

    • Author(s)
      Nakamura, Y., Ohta, R., Yamaguchi, N., et al.
    • Journal Title

      The Japanese Journal of Taste and Smell Research 12(3)

      Pages: 247-250

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2008-05-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi