Unraveling molecular mechanisms to recreate 3D organ architecture

2009 Fiscal Year Final Research Report

• Project/Area Number: 17GS0318
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: Kyoto University
• Principal Investigator: AGATA Kiyokazu Kyoto University, 大学院・理学研究科, 教授 (70167831)
• Project Period (FY): 2005 – 2009
• Keywords: 幹細胞 / 位置情報 / 器官形成

Research Abstract

We investigated the mechanisms underlying organ regeneration from pluripotent stem cells in planarians. We applied these strategies to organ formation from mouse ES cells in vitro.

Research Products

• [Journal Article] Different requirements for post-transcriptional regulators and translational initiation factors during planarian regeneration and stem cell maintenance. (2010)
  • Author(s): L. Rouhana, N. Shibata, K. Agata
  • Journal Title: Dev. Biol. 341 Pages: 429-443
  • Peer Reviewed
• [Journal Article] Analysis of motor function modulated by cholonergic neurins in planarian Dugesia japonica (2010)
  • Author(s): K. Nishimura, Y. Kitamura, T. Taniguchi, K. Agata
  • Journal Title: Neuroscience 168 Pages: 18-30
  • Peer Reviewed
• [Journal Article] Single-cell gene profiling of planarian stem cells using fluorescent activated cell sorting and its "index sorting" function for stem cell research. (2010)
  • Author(s): T. Hayashi, N. Shibata, R. Okumura, T. Kudome, O. Nishimura, H. Taru, K. Agata
  • Journal Title: Dev. Growth Differ. 52 Pages: 131-144
  • Peer Reviewed
• [Journal Article] Cellular and molecular dissection of pluripotent adult somatic stem cells in planarians. (2010)
  • Author(s): N. Shibata, L. Rouhana, K. Agata
  • Journal Title: Dev. Growth Differ. 52 Pages: 27-41
  • Peer Reviewed
• [Journal Article] Planarian Hedgehog/Patched establishes anterior-posterior polarity by regulating Wnt signaling. (2009)
  • Author(s): S. Yazawa, Y. Umesono, T. Hayashi, H. Tarui, K. Agata
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 22329-22334
  • Peer Reviewed
• [Journal Article] Planarians maintain a constant ratio of different cell types during changes in body size by using the stem cell system (2009)
  • Author(s): H. Takeda, K. Nishimura, K. Agata
  • Journal Title: Zool. Sci. 26 Pages: 805-813
  • Peer Reviewed
• [Journal Article] Identification of a novel planarian G-protein-coupled receptor that responds to serotonin in Xenopus laevis oocytes K. Nishimura (2009)
  • Author(s): K. Unemura, J. Tsushima, Y. Yamauchi, J. Otomo, T. Taniguchi, S. Kaneko, K. Agata, Y. Kitamura
  • Journal Title: Biol. Pharm. Bull. 32 Pages: 1672-1677
  • Peer Reviewed
• [Journal Article] Evolution and regeneration of the planarian central nervous system (2009)
  • Author(s): Y. Umesono, K. Agata
  • Journal Title: Growth Differ. 51 Pages: 185-195
  • Peer Reviewed
• [Journal Article] Charactization of tyramine β-hydroxylase in planarian Dugesia japonica, Cloning and expression. (2008)
  • Author(s): K. Nishimura, Y. Kitamura, T. Inoue, Y. Umesono, K. Yoshimoto, T. Taniguchi, K. Agata
  • Journal Title: Neurochemi. Inter. 53 Pages: 184-192
  • Peer Reviewed
• [Journal Article] Identification of glutamic acid decarboxylase gene and distribution of GABAergic nervous system in the planarian Dugesia laponica. (2008)
  • Author(s): K. Nishimura, Y. Kitamura, Y. Umesono, K. Takeuchi. K. Takata, T. Taniguchi, K. Agata
  • Journal Title: Neuroscience 153 Pages: 1103-1114
  • Peer Reviewed
• [Journal Article] Expression and functional analysis of musashi-like genes in planarian CNS regeneration. (2008)
  • Author(s): S. Higuchi, T. Hayashi, H. Tarui, O. Nishimura, K. Nishimura, N. Shibata, H. Sakamoto, K. Agata
  • Journal Title: Mech. Dev. 25 Pages: 631-645
  • Peer Reviewed
• [Journal Article] Brain regeneration from pluripotent stem cells in planarian. (2008)
  • Author(s): K. Agata, Y. Umesono
  • Journal Title: Phil. Trans. R. Soc. Lond. B. Biol. Sci. 363 Pages: 2071-2078
  • Peer Reviewed
• [Journal Article] Identification of glutamic acid decarboxylase gene and distribution of GABAergic nervous system in the planarian Dugesia japonica. (2008)
  • Author(s): K. Nishimura, Y. Kitamura, Y. Umesono, K. Takeuchi, K. Takata, T. Taniguchi, K. Agata
  • Journal Title: Neurosci. 153 Pages: 1103-1114
  • Peer Reviewed
• [Journal Article] Characterization and categorization of FACS-sorted planarian stem cells by ultrastructural analysis. (2007)
  • Author(s): S. Higuchi, T. Hayashi, I. Hori, N. Shibata, H. Sakamot, K. Agata
  • Journal Title: Dev. Growth Differ. 49 Pages: 571-581
  • Peer Reviewed
• [Journal Article] Wnt signaling is required for antero-posterior patterning of the planarian brain. (2007)
  • Author(s): C. Kobayashi, K. Ogawa, K. Agata
  • Journal Title: Dev. Biol. 306 Pages: 714-724
  • Peer Reviewed
• [Journal Article] Clathrin-mediated endocytic signal is required for the regeneration as well as homeostasis of planarian CNS. (2007)
  • Author(s): T. Inoue, T. Hayashi, K. Takechi, K. Agata
  • Journal Title: Development 134 Pages: 1679-1689
  • Peer Reviewed
• [Journal Article] Reconstruction of dopaminergic neural network and locomotion function in planarian regenerates. (2007)
  • Author(s): K. Nishimura, Y. Kitamura, T. Inoue, Y. Umesono, S. Sano, K. Yoshimoto, M. Inden, K. Takata, T. Taniguchi, S. Shimohama, K. Agata
  • Journal Title: Dev. Neurobiol. 67 Pages: 1059-1078
  • Peer Reviewed
• [Journal Article] Regeneration-dependent conditional gene knockdown (Readyknock) in planarian: Demonstration of requirement for Djsnap-25 expression in the brain for negative phototactic behavior. (2007)
  • Author(s): T. Takano, J. Pulvers, T. Inoue, H. Tarui, H. Sakamoto, K. Agata, Y. Umesono
  • Journal Title: Dev. Growth Differ. 49 Pages: 383-394
  • Peer Reviewed
• [Journal Article] Unifying principles of regeneration I: epimorphosis versus morphallaxis. (2007)
  • Author(s): K. Agata, Y. Saito, E. Nakajima
  • Journal Title: Dev. Growth Differ. 49 Pages: 73-78
  • Peer Reviewed
• [Journal Article] Two different evolutionary origins of stem cell systems and their molecular basis (2006)
  • Author(s): K. Agata, E. Nakajima, N. Funayama, N. Shibata, Y. Saito, Y. Umesno
  • Journal Title: Semin. Cell Dev. Biol. 17 Pages: 503-509
  • Peer Reviewed
• [Journal Article] Structure and function of primitive immunoglobulin superfamily neural cell adhesion molecules: a lesson from studies on planarian (2006)
  • Author(s): E. Fusaoka, T. Inoue, K. Mineta, K. Agata, K. Takeuchi
  • Journal Title: Genes to Cells 11 Pages: 541-555
  • Peer Reviewed
• [Journal Article] Isolation of planarian X-ray-sensitive stem cells by fluorescence-activated cell sorting (2006)
  • Author(s): T. Hayashi, M. Asami, S. Higuchi, N. Shibata, K. Agata
  • Journal Title: Dev. Growth Differ. 48 Pages: 371-379
• [Journal Article] Neural projections in planarian brain revealed by fluorescent dye tracing. (2005)
  • Author(s): K. Okamoto, K. Takeuchi, K. Agata
  • Journal Title: Zool. Sci. 22 Pages: 535-546
  • Peer Reviewed
• [Presentation] Regeneration in planarian (2009)
  • Author(s): K. Agata
  • Organizer: Frontiers in Stem Cells and Regeneration Advanced Training Course
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2009-10-07
• [Presentation] Brain regeneration from pluripotent stem cells in planarian German (2009)
  • Author(s): K. Agata
  • Organizer: Dev. Biol. Meeting
  • Place of Presentation: Hannover Germany
  • Year and Date: 2009-03-28
• [Presentation] Maintenance and regulation of planarian pluripotent stem cells (2008)
  • Author(s): K. Agata
  • Organizer: 23th Naito Conference
  • Place of Presentation: Shonan Village Centaer, Kanagawa Japan
  • Year and Date: 2008-11-12
• [Presentation] Brain regeneration from pluripotent stem cells in planarian (2007)
  • Author(s): K. Agata
  • Organizer: Biology Seminar
  • Place of Presentation: UC Berkeley USA
  • Year and Date: 2007-10-31
• [Book] Planaria: A Model for Drug Action and Abuse (Brain and neural networks) (2008)
  • Author(s): K. Nishimura, H. Yamamoto, Y. Kitamura, K. Agata
  • Total Pages: 12
  • Publisher: LANDES BIOSCIENCE
• [Book] Stem Cells: From Hydra To Man (Stem cells in planarian.) (2008)
  • Author(s): K. Agata
  • Total Pages: 59-74
  • Publisher: Springer
• [Book] Planarian nervous system (2008)
  • Author(s): H. Takeda, K. Nishimura, K. Agata
  • Total Pages: 5558
  • Publisher: Scholarpedia