Establishment of "Contractile Muscle Cell Culture System" using Human-derived Satellite Cells

2019 Fiscal Year Final Research Report

• Project/Area Number: 17H02076
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tohoku University
• Principal Investigator: Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
• Co-Investigator(Kenkyū-buntansha): 萩原 嘉廣 東北大学, 医学系研究科, 准教授 (90436139)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 骨格筋 / 筋細胞 / 運動 / 培養システム / 電気パルス / 筋疾患 / マイオカイン

Outline of Final Research Achievements

This study aimed to establish an “in vitro exercise model” using human-derived muscle cells that often exhibit relatively poor contractile activity. By employing biomedical engineering techniques along with co-culture systems, we successfully established “in vitro exercise models” using (1) hybrid myotubes comprised of human myoblasts and murine C2C12 myoblasts as well as those using (2) murine 3T3L1 cells as a feeder for supporting development of human myotube contractile ability, that both exhibit vigorous contractile activity in response to electric pulse stimulation (EPS). Species-specific analyses allow us to separately evaluate contraction-inducible gene expressions and myokine secretions from human and mouse constituents. We also fabricated a culture insert chamber that one can efficiently deliver EPS to the myotubes. Thus, these inventions would allow us to maximize the use of the human myocytes obtained from biopsy samples, a very limited resource.

Free Research Field

分子細胞生理学

Academic Significance and Societal Importance of the Research Achievements

本研究課題の達成により確立されたヒト由来筋細胞を用いた「運動できる培養筋細胞系」を活用することにより、立ち後れている骨格筋を治療標的とする創薬研究に対して大きな貢献をもたらすことができると期待される。また、健常者のみならず、筋疾患患者より採取したヒト筋細胞をこの特殊培養系にて人為的な任意の収縮運動を負荷することにより、収縮能力の獲得過程や、運動応答性に惹起される各種の高次筋機能（マイオカイン類発現調節、アダプテーションや代謝能亢進など）を培養系において評価可能な「筋細胞高次機能診断システム」の将来的な構築が可能となった。