2017 Fiscal Year Annual Research Report
Plasma Biochemistry and Metabolic Profiles of Cells Interacted with Non-thermal Plasmas
| Project/Area Number |
17H02805
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| Research Institution | Nagoya University |
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Principal Investigator |
石川 健治 名古屋大学, 工学研究科, 特任教授 (60417384)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | プラズマ / プラズマ医療科学 / 生体分子 / 代謝 |
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| Outline of Annual Research Achievements |
放電プラズマを生体に作用させ，ヒト細胞への抗腫瘍作用や農産物の成長促進，酵素活性といった生物学，農学的な応用が盛んがであるにもかかわらず，バイオプラズマ応用でのプラズマと生体との相互作用は未知であり，その解析が急務である．研究実施者は，プラズマ生成するイオン，ラジカル，光といった個々の反応種の作用をその場で実時間に観察してプラズマと生体の相互作用の解明に取り組んでいる．本研究では，プラズマと生体内のフリーラジカルの挙動が細胞内化学反応（代謝）の変動の検出に発展させ，キャピラリー電気泳動質量分析検出による解析を進めている．本年度は，（１）プラズマと液体の相互作用の理解：プラズマが合成する化学種のうち代謝影響の候補物質を各種有機物分析手法で進めてきた．複雑な生体エネルギー代謝系に焦点をおき，プラズマ生成物の違いによる代謝物変動のプロファイルを始めることができた．（２）プラズマ代謝経路マップの作成：細胞内酵素反応をプラズマ刺激入力に対する細胞システムの応答ととらえ，プラズマ作用の候補物質の作用，機能を解析することを始めることもできた．プラズマと有機物の反応を解析するため，核磁気共鳴法をもちいてプラズマが影響して生成される合成物の分析を進めた．（３）プラズマ代謝制御：代謝機能を制御する方法を確立する，基盤知識が集積しつつある．最終目標とする自然物と人工物の融合利用に向けた「プラズマ生物科学」の創成に向け，着実に成果を得ることができた．
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
プラズマが合成する有機物の解析を，キャピラリー電気泳動質量分析法，核磁気共鳴法，レーザー脱離イオン化時間飛行型質量分析法で行うことができたため
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| Strategy for Future Research Activity |
プラズマが合成する有機物には比較的短寿命で構造変化してしまう化学種があるため，核磁気共鳴法を世界に先んじてプラズマ装置と結び付けダイナミクスな解析を始める
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[Journal Article] Free radical generation by non-equilibrium atmospheric pressure plasma in alcohol-water mixtures. An EPR-spin trapping study2018
Author(s)
Hidefumi Uchiyama, Qing-Li Zhao, Gabor Andocs, Nobuyuki Nojima, Keigo Takeda, Murali Krishna, Yuji Matsuya, Kenji Ishikawa, Masaru Hori, Kyo Noguchi, and Takashi Kondo
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Journal Title
J. Phys.D: Appl. Phys.
Volume: 51
Pages: 26201
DOI
Peer Reviewed / Int'l Joint Research
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[Presentation] Inhomogeneity of nitrite anion concentrations in depth and time by convection during and after plasma-liquid interactions2018
Author(s)
Timothy R. Brubaker, Kenji Ishikawa, Keigo Takeda, Jun-Seok Oh, Masafumi Ito, Hiroki Kondo, Hiroshi Hashizume, Hiromasa Tanaka, Sean D. Knecht, Sven G. Bil?n, and Masaru Hori
Organizer
2nd International Workshop on Plasma Agriculture, March 9-12, 2018, Takayama, Japan
Int'l Joint Research
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[Presentation] プラズマ活性培養液処理した細胞内酸化窒素活性種(RONS)の解析2017
Author(s)
石川 健治, 田中 宏昌, 橋爪 博司, 竹田 圭吾, 太田 貴之, 伊藤 昌文, 中村 香江, 梶山 広明, 吉川 史隆, 豊國 伸哉, 水野 正明, 堀 勝
Organizer
第７０回日本酸化ストレス学会学術集会 於：つくば国際会議場
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[Presentation] Programmed process for synthesis of calcium oxalate crystals in a bufferred glucose solution by irradiation with non-equilibrium atmospheric-pressure plasma2017
Author(s)
Kenji Ishikawa, Naoyuki Kurake, Hiromasa Tanaka, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Yoko Yamanishi, Yuzuru Ikehara, and Masaru Hori
Organizer
iPlasmaNano-VIII July 2-6, 2017. the University of Antwerp, Antwerp, Belgium.
Int'l Joint Research
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[Presentation] Observation of intracellular-molecular changes in plasma-irradiated cells using multiplex coherent anti-Stokes Raman scattering microscopy2017
Author(s)
Takayuki Ohta, Ryo Furuta, Naoyuki Kurake, Kenji Ishikawa, Keigo Takeda, Hiroshi Hashizume, Hiroki Kondo, Masafumi Ito, and Masaru Hori
Organizer
AEPSE 2017, Jeju International Convention Center, Jeju Island, Republic of Korea, September 11-15, 2017
Int'l Joint Research
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[Presentation] Ambient control during preparations of the plasma-activated medium (PAM) for antitumor effects2017
Author(s)
Yugo Hosoi, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
Organizer
AEPSE 2017, Jeju International Convention Center, Jeju Island, Republic of Korea, September 11-15, 2017
Int'l Joint Research
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[Presentation] Measurements of reactive species and VUV radiators generated by AC excited atmospheric pressure Ar plasma jet in open air2017
Author(s)
Keigo Takeda, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
Organizer
AEPSE 2017, Jeju International Convention Center, Jeju Island, Republic of Korea, September 11-15, 2017
Int'l Joint Research
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[Presentation] Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Observations of HeLa Cells Cultured in Non-equilibrium Atmospheric Pressure-Plasma-Activated Medium (PAM)2017
Author(s)
Kenji Ishikawa, Ryo Furuta, Keigo Takeda, Takayuki Ohta, Masafumi Ito, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, Masaru Hori
Organizer
AVS - 4th International Symposium and Exhibition, October 29-November 03, 2017. Tampa, Florida, USA
Int'l Joint Research
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[Presentation] Intracellular responses during incubation in plasma-activated cell culture medium (PAM)2017
Author(s)
Kenji Ishikawa, Ryo Furuta, Naoyuki Kurake, Keigo Takeda, Hiroshi Hashizume, Hiromasa Tanaka, Takayuki Ohta, Masafumi Ito, Hiroki Kondo, Makoto Sekine, and Masaru Hori
Organizer
27th annual meeting of MRS-J, Yokohama, Japan, December 5-7, 2017
Int'l Joint Research
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[Presentation] Effects of dielectric walls on atmospheric-pressure plasma discharges2017
Author(s)
Kaede Katsuno, Kenji Ishikawa, Takayoshi Tsutsumi, Keigo Takeda, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
Organizer
APSPT-10 - 10th Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology, December 15-17, 2017. Taiwan.
Int'l Joint Research
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