Plasma Biochemistry and Metabolic Profiles of Cells Interacted with Non-thermal Plasmas

2018 Fiscal Year Annual Research Report

• Project/Area Number: 17H02805
• Research Institution: Nagoya University
• Principal Investigator: 石川 健治 名古屋大学, 低温プラズマ科学研究センター, 特任教授 (60417384)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: プラズマ / プラズマ医療科学 / 生体分子 / 代謝

Outline of Annual Research Achievements

放電プラズマを生体に作用させ，ヒト細胞への抗腫瘍作用や農産物の成長促進，酵素活性といった生物学，農学的な応用が盛んがであるにもかかわらず，バイオプラズマ応用でのプラズマと生体との相互作用は未知であり，その解析が急務である．研究実施者は，プラズマ生成するイオン，ラジカル，光といった個々の反応種の作用をその場で実時間に観察してプラズマと生体の相互作用の解明に取り組んでいる．本研究では，プラズマと生体内のフリーラジカルの挙動が細胞内化学反応（代謝）の変動の検出に発展させ，キャピラリー電気泳動質量分析検出による解析を進めている．本年度は，（１）プラズマと液体の相互作用の理解：プラズマが合成する化学種のうち代謝影響の候補物質を各種有機物分析手法で進めてきた．複雑な生体エネルギー代謝系に焦点をおき，プラズマ生成物の違いによる代謝物変動のプロファイルを始めることができた．（２）プラズマ代謝経路マップの作成：細胞内酵素反応をプラズマ刺激入力に対する細胞システムの応答ととらえ，プラズマ作用の候補物質の作用，機能を解析することを始めることもできた．プラズマと有機物の反応を解析するため，核磁気共鳴法をもちいてプラズマが影響して生成される合成物の分析を進めた．（３）プラズマ代謝制御：代謝機能を制御する方法を確立する，基盤知識が集積しつつある．最終目標とする自然物と人工物の融合利用に向けた「プラズマ生物科学」の創成に向け，着実に成果を得ることができた．

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

プラズマが合成する有機物の解析を，キャピラリー電気泳動質量分析法，核磁気共鳴法，レーザー脱離イオン化時間飛行型質量分析法で行うことができたため

Strategy for Future Research Activity

プラズマが合成する有機物には比較的短寿命で構造変化してしまう化学種があるため，核磁気共鳴法を世界に先んじてプラズマ装置と結び付けダイナミクスな解析を始める

Research Products

• [Journal Article] Nonthermal plasma-activated medium (PAM) modified metabolomic profiles in glycolysis of U251SP glioblastoma (2019)
  • Author(s): Naoyuki Kurake, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Kae Nakamura, Hiroaki Kajiyama, Shinya Toyokuni, Fumitaka Kikkawa, Masaaki Mizuno, and Masaru Hori
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 662 Pages: 83-92
  • DOI: 10.1016/j.abb.2018.12.001
  • Peer Reviewed
• [Journal Article] Systematic diagnostics of the electrical, optical, and physicochemical characteristics of low-temperature atmospheric-pressure helium plasma sources (2019)
  • Author(s): Keigo Takeda, Hiromasa Yamada, Kenji Ishikawa, Hajime Sakakita, Jaeho Kim, Masashi Ueda, Jun-ichiro Ikeda, Yoshihiro Akimoto, Yosky Kataoka, Naoaki Yokoyama, Yuzuru Ikehara, and Masaru Hori
  • Journal Title: Journal of Physics D:Applied Physics Volume: 52 Pages: 165202
  • DOI: 10.1088/1361-6463/aaff44
  • Peer Reviewed
• [Journal Article] Effect of electrical stimulation on proliferation and bone-formation by osteoblast-like cells cultured on carbon nanowall scaffolds (2019)
  • Author(s): Tomonori Ichikawa, Suiki Tanaka, Hiroki Kondo, Kenji Ishikawa, Takayoshi Tsutsumi, Makoto Sekine, and Masaru Hori
  • Journal Title: Applied Physics Express Volume: 12 Pages: 025006
  • DOI: 10.7567/1882-0786/aaf469
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of non-thermal plasma-induced effects in cancer cells (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Shinya Toyokuni, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori
  • Journal Title: Biological Chemistry Volume: 400 Pages: 87-91
  • DOI: 10.1515/hsz-2018-0199
  • Peer Reviewed
• [Journal Article] New hopes for plasma-based cancer treatment (2018)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Shinya Toyokuni, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori
  • Journal Title: Plasma Volume: 1 Pages: 150-155
  • DOI: 10.3390/plasma1010014
  • Peer Reviewed
• [Journal Article] Cytotoxic effects of plasma-irradiated fullerenol (2018)
  • Author(s): Daiki Kanno, Hiromasa Tanaka, Kenji Ishikawa, Hiroshi Hashizume, and Masaru Hori
  • Journal Title: Journal of Physics D: Applied Physics Volume: 51 Pages: 375401
  • DOI: 10.1088/1361-6463/aad510
  • Peer Reviewed
• [Journal Article] Cell Deposition Microchip with Micropipette Control over Liquid Interface Motion (2018)
  • Author(s): Daisuke Onoshima, Yuya Hattori, Hiroshi Yukawa, Kenji Ishikawa, Masaru Hori, and Yoshinobu Baba
  • Journal Title: Cell Medicine Volume: 10 Pages: 1-5
  • DOI: 10.1177/2155179017733152
  • Peer Reviewed
• [Journal Article] Low-autofluorescence fluoropolymer membrane filters for cell filtration (2018)
  • Author(s): Naoto Kihara, Daiki Kuboyama, Daisuke Onoshima, Kenji Ishikawa, Ryohei Koguchi, Hiromasa Tanaka, Naoya Ozawa, Tetsunari Hase, Hiroshi Yukawa, Hidefumi Odaka, Yoshinori Hasegawa, Yoshinobu Baba, and Masaru Hori
  • Journal Title: Japanese Journal of Applied Physics Volume: 57 Pages: 06JF03
  • DOI: 10.7567/JJAP.57.06JF03
  • Peer Reviewed
• [Presentation] Low Temperature Plasma Surface Interactions Among Multiple Phase (2019)
  • Author(s): Kenji Ishikawa, Masaru Hori
  • Organizer: 5th Asian Workshop on Applied Plasma Science and Engineering, Kuala Lumpur, Malaysia
  • Int'l Joint Research / Invited
• [Presentation] Mechanism of Selective Killing of Glioblastoma to Astrocytes in PlasmaActivated Lactate (2019)
  • Author(s): Yugo Hosoi, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Masaaki Mizuno, Fumitaka Kikkawa and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Aqueous Reactions in Non-equilibrium Atmospheric Pressure Plasma-activated Ringer s Lactate Solutions (2019)
  • Author(s): Yang Liu, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Takyoshi Tsutsumi, Fumitaka Kikkawa, Kae Nakamura, Masaaki Mizuno, Shinya Toyokuni, Hiroki Kondo, and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Intracellular Reactive Oxygen Species Responses of HeLa Cells Treated with PlasmaActivated Organics (2019)
  • Author(s): Shogo Maeda, Yugo Hosoi, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Regulation of Cell Proliferation Changing Duty Ratio of an Electrical Stimulation on Carbon Nanowalls Scaffold (2019)
  • Author(s): Tomonori Ichikawa, Hiroki Kondo, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Kenji Ishikawa, Makoto Sekine and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Morphological Dynamics of Dying Cells Incubated in Plasma-Activated Medium (2019)
  • Author(s): Nanami Ito, Maho Yamada, Yugo Hosoi, Hiromasa Tanaka, Kenji Ishikawa and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Cytotoxicity of Plasma-Activated Glucose and Amino Acids (2019)
  • Author(s): Maho Yamada, Nanami Ito, Yugo Hosoi, Hiromasa Tanaka, Kenji Ishikawa and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Intracellular responses in apoptotic cells to reactive species in plasma treated liquids (2018)
  • Author(s): K. Ishikawa, H. Tanaka, T. Kondo, S. Toyokuni, F. Kikkawa, M. Mizuno, and M. Hori
  • Organizer: 7th International Conference on Plamsa Medicine (ICPM7), Drexekl University Philadelphia, USA
  • Int'l Joint Research / Invited
• [Presentation] Low temperature plasma surface interactions for their future basic researches and applications (2018)
  • Author(s): K. Ishikawa, M. Itou, and M. Hori
  • Organizer: 22nd International Conference Gas Discharges and Their Applications, Serbia
  • Int'l Joint Research / Invited
• [Presentation] Mechanisms of plasma jets impinging upon liquids (2018)
  • Author(s): T. Brubaker, K. Ishikawa, H. Hashizume, H. Tanaka, T. Tsutsumi, H. Kondo, S. Knecht, S. Bilen, and M. Hori
  • Organizer: 7th International Conference on Plamsa Medicine (ICPM7), Drexekl University Philadelphia, USA
  • Int'l Joint Research
• [Presentation] Similarities and Differences in Gene Transcription in Plasma-activated Medium Treated and Plasma-activated Ringer’s Lactate Solution Treated Glioblastomas (2018)
  • Author(s): H. Tanaka, M. Mizuno, K. Ishikawa, K. Takeda, H. Hashizume, H. Kondo, M. Sekine, K. Nakamura, H. Kajiyama, S. Akiyama, S. Maruyama, S. Toyokuni, F. Kikkawa, M. Hori
  • Organizer: 7th International Conference on Plamsa Medicine (ICPM7), Drexekl University Philadelphia, USA
  • Int'l Joint Research
• [Presentation] Biological Effects of Plasma-Irradiated Oraganic Molecules in Plasma-Treated Liquids (2018)
  • Author(s): Kenji Ishikawa, Y. Hosoi, D.Kanno, Y. Kurokawa, H. Tanaka, M. Mizuno, F.Kikkawa, M.Hori
  • Organizer: AVS 65th INTERNATIONAL SYMPOSIUM & EXHIBITION 2018, Long Beach, California, USA
  • Int'l Joint Research
• [Presentation] Nonequilibrium atmospheric pressure plasma activates organic substances for antitumor effects (2018)
  • Author(s): Kenji Ishikawa, Yugo Hosoi, Hiromasa Tanaka, Hiroshi Hashizume, Shinya Toyokuni, Fumitaka Kikkawa, Masaaki Mizuno, and Masaru Hori
  • Organizer: 40th International Symposium on Dry Process (DPS2018), Toyoda Auditorium, Nagoya University, Nagoya, Aichi, Japan
  • Int'l Joint Research
• [Presentation] Anti-proliferative effect of glyoxylate generated in plasma-activated lactate solution (2018)
  • Author(s): Y. Hosoi, K. Ishikawa, H. Tanaka, H. Hashizume, F. Kikkawa, M. Mizuno and M. Hori
  • Organizer: 28th Materials Research Society of Japan (MRS-J) , Kitakyusyu International Conference Center, Fukuoka, Japan
  • Int'l Joint Research
• [Presentation] Cell response mechanisms of HeLa cells cultured with plasma-activated lactate Ringer’s solution (PAL) (2018)
  • Author(s): S. Maeda, Y. Hosoi, K. Ishikawa, H. Hashizume, H. Tanaka, T. Takayoshi, H. Kondo, M. Sekine, and M.Hori
  • Organizer: 28th Materials Research Society of Japan (MRS-J) , Kitakyusyu International Conference Center, Fukuoka, Japan
  • Int'l Joint Research
• [Book] Chapter 2 Physical and Chemical Basis of Nonthermal Plasma, In "Plasma Medical Science" (2018)
  • Author(s): Kenji Ishikawa et al.
  • Total Pages: 103
  • Publisher: Academic Press
  • ISBN: 9780128150047
• [Remarks] Homepage
  • URL: http://www.plasma.engg.nagoya-u.ac.jp/ishikawa/index.php/Home